Thiamin biosynthesis in prokaryotes.

Abstract:

:Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and four are kinase genes (thiD, thiM, thiL, and pdxK). The specific reactions catalyzed by ThiEF, Dxs, ThiDM, ThiL, and PdxK have been reconstituted in vitro and ThiS thiocarboxylate has been identified as the sulfur source. The X-ray structures of thiamin phosphate synthase and 5-hydroxyethyl-4-methylthiazole kinase have been completed. The genes coding for the thiamin transport system (thiBPQ) have also been identified. Remaining problems include the cloning and characterization of thiK (thiamin kinase) and the gene(s) involved in the regulation of thiamin biosynthesis. The specific reactions catalyzed by ThiC (pyrimidine formation), and ThiGH and ThiI (thiazole formation) have not yet been identified.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Begley TP,Downs DM,Ealick SE,McLafferty FW,Van Loon AP,Taylor S,Campobasso N,Chiu HJ,Kinsland C,Reddick JJ,Xi J

doi

10.1007/s002030050713

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

293-300

issue

5

eissn

0302-8933

issn

1432-072X

journal_volume

171

pub_type

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