Abstract:
:Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and four are kinase genes (thiD, thiM, thiL, and pdxK). The specific reactions catalyzed by ThiEF, Dxs, ThiDM, ThiL, and PdxK have been reconstituted in vitro and ThiS thiocarboxylate has been identified as the sulfur source. The X-ray structures of thiamin phosphate synthase and 5-hydroxyethyl-4-methylthiazole kinase have been completed. The genes coding for the thiamin transport system (thiBPQ) have also been identified. Remaining problems include the cloning and characterization of thiK (thiamin kinase) and the gene(s) involved in the regulation of thiamin biosynthesis. The specific reactions catalyzed by ThiC (pyrimidine formation), and ThiGH and ThiI (thiazole formation) have not yet been identified.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Begley TP,Downs DM,Ealick SE,McLafferty FW,Van Loon AP,Taylor S,Campobasso N,Chiu HJ,Kinsland C,Reddick JJ,Xi Jdoi
10.1007/s002030050713keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
293-300issue
5eissn
0302-8933issn
1432-072Xjournal_volume
171pub_type
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