Abstract:
:CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28 degrees C; but at 4 degrees C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was transiently enhanced when cells were shifted from 22 to 4 degrees C indicating that AAT is cold-inducible. Complementation of the mutated AAT transformed CSM2 from a cold-sensitive phenotype to a cold-resistant phenotype like the wild-type cells, thus providing evidence for the first time that AAT is required for low-temperature growth.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Sundareswaran VR,Singh AK,Dube S,Shivaji Sdoi
10.1007/s00203-010-0591-7subject
Has Abstractpub_date
2010-08-01 00:00:00pages
663-72issue
8eissn
0302-8933issn
1432-072Xjournal_volume
192pub_type
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