Aspartate aminotransferase is involved in cold adaptation in psychrophilic Pseudomonas syringae.

Abstract:

:CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28 degrees C; but at 4 degrees C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was transiently enhanced when cells were shifted from 22 to 4 degrees C indicating that AAT is cold-inducible. Complementation of the mutated AAT transformed CSM2 from a cold-sensitive phenotype to a cold-resistant phenotype like the wild-type cells, thus providing evidence for the first time that AAT is required for low-temperature growth.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Sundareswaran VR,Singh AK,Dube S,Shivaji S

doi

10.1007/s00203-010-0591-7

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

663-72

issue

8

eissn

0302-8933

issn

1432-072X

journal_volume

192

pub_type

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