Abstract：
:Methanotrophs are ubiquitous in the environment and play an important role in mitigating global warming due to methane. They are also potentially interesting for industrial applications such as production of bulk chemicals or bioremediation. The first step in the oxidation of methane is the conversion to methanol by methane monooxygenase, the key enzyme, which exists in two forms: the cytoplasmic, soluble methane monooxygenase (sMMO) and the membrane-bound, particulate methane monooxygenase (pMMO). This paper reviews the biochemistry and molecular biology of both forms of MMO. In the past few years there have been many exciting new findings. sMMO components have been expressed in heterologous and homologous hosts. The pMMO has been purified and biochemically studied in some detail and the genes encoding the pMMO have been sequenced. Copper ions have been shown to play a key role in regulating the expression of both MMO enzyme complexes. We also present a model for copper regulation based on results from Northern analysis, primer-extensions and new sequence data, and raise a number of unanswered questions for future studies.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Murrell JC,Gilbert B,McDonald IRdoi
10.1007/s002030000158keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
325-32issue
5-6eissn
0302-8933issn
1432-072Xjournal_volume
173pub_type
杂志文章,评审abstract：:Fructose transport was not apparently affected in a number of Pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. Fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428362
更新日期：1975-01-01 00:00:00
abstract：:Given the serious threat of foodborne multidrug-resistant bacteria to animals and humans, finding an effective antibacterial compound has always been an important topic for scientists. Here, from the soil of Changbaishan, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus. Nr genome da...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02102-8
更新日期：2020-11-04 00:00:00
abstract：:The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429336
更新日期：1977-03-01 00:00:00
abstract：:The presence of one periplasmic [NiFe] hydrogenase, one periplasmic [Fe] hydrogenase, and one cytoplasmic NADP-reducing hydrogenase has been previously established in Desulfovibrio fructosovorans. In the present work, marker-exchange mutagenesis was performed to determine the function of the tetrameric NADP-reducing h...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050414
更新日期：1997-01-01 00:00:00
abstract：:The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0119-3
更新日期：2006-07-01 00:00:00
abstract：:In studies on the membranlytic action of various saponins on mycelium of Botrytis cinerea and Rhizoctonia solani digitonin, alpha-hederin and tomatin caused considerable leakage of free amino acids, while aescin and theasaponin were less effective. Cyclamin significantly damaged cell membranes of R. solani, but did no...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447315
更新日期：1975-06-22 00:00:00
abstract：:Histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. An antigenic surface layer has been visualized in the cell wall of immunostained organisms. This layer was not seen in samples prepared by standard electro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00693397
更新日期：1985-08-01 00:00:00
abstract：:Both the changes in the activities of nitrogenase, glutamine synthetase and glutamate dehydrogenase and in the extracellular and intracellular NH4+ concentrations were investigated during the transition from an NH4+ free medium to one containing NH4+ ions for a continuous culture of Azotobacter vinelandii. If added in...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447319
更新日期：1975-06-22 00:00:00
abstract：:Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mas...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00262202
更新日期：1995-01-01 00:00:00
abstract：:The synthesis of beta-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-D-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the prese...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447154
更新日期：1975-11-07 00:00:00
abstract：:Nitrogen (N) is among the most important macro-nutrients significantly affecting plant growth and yield production. Accordingly, N must be supplied adequately so that optimum amounts of yield are resulted. There are different ways of supplying N to the plant including the use of chemical and biological fertilization. ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-010-0657-6
更新日期：2011-02-01 00:00:00
abstract：:Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and f...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050713
更新日期：1999-04-01 00:00:00
abstract：:Bacillus cereus F4430/73 produced the highest levels of hemolysin BL (HBL) when grown under anaerobiosis in MOD medium. Anaerobic cells grown in a chemostat at low specific growth rate (0.1-0.2 h(-1)) expressed up to sevenfold more HBL than did cells held at a faster growth rate. At 0.2 h(-1), the presence of 90 mM gl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0688-y
更新日期：2004-09-01 00:00:00
abstract：:Methanobacterium thermoautotrophicum growing on H2 plus CO2 as sole carbon and energy source was found to contain acetate thiokinase (Acetyl CoA synthetase; EC 6.2.1.1); Acetate + ATP + CoA leads to Acetyl CoA + AMP + PPi. The apparent Km value for acetate was 40 microM. Acetate kinase (EC 2.7.2.1) and phosphotransace...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406167
更新日期：1980-12-01 00:00:00
abstract：:Klebsiella pneumoniae NCTC 418 was cultured aerobically in chemostat cultures (D = 0.3 h-1; 35 degrees C) under respectively carbon-, phosphate-, potassium-, sulphate-, and ammonia-limited conditions with glucose as the sole carbon and energy source. The effect of the external pH value on glucose metabolism and on the...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413140
更新日期：1989-01-01 00:00:00
abstract：:The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1222-8
更新日期：2016-09-01 00:00:00
abstract：:In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01801-1
更新日期：2020-05-01 00:00:00
abstract：:The mitochondrial genome (mtDNA) of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae, with a total size of 24,673 bp, was one of the smallest known mtDNAs of Pezizomycotina. It contained the 14 typical genes coding for proteins related to oxidative phosphorylation, the two rRNA genes, a single intron...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0104-x
更新日期：2006-06-01 00:00:00
abstract：:We cloned the Kluyveromyces lactis KlNTH1 gene, which encodes neutral trehalase. It showed 65.2% and 68.5% identity at nucleotide and amino acid sequence level, respectively, with the Saccharomyces cerevisiae NTH1 gene. Multiple alignment of the predicted trehalase protein sequences from yeasts, bacteria, insects, and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050436
更新日期：1997-04-01 00:00:00
abstract：:Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Cal...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413014
更新日期：1984-06-01 00:00:00
abstract：:An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FB(T), was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0521-z
更新日期：2003-04-01 00:00:00
abstract：:All Serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. DNA fragments of the two hemolysin genes hybridized with the chromosomal DNA of S. marcescens, S. liquefaciens, S. kiliensis, S. grimesii, S. proteamaculans, S. plym...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248958
更新日期：1990-01-01 00:00:00
abstract：:Electron microscopic studies emphasized that the protein-E-specific transmembrane tunnel structure, which permeabilizes Escherichia coli, is not randomly distributed over the cell envelope but is restricted to areas of potential division sites. These sites were located predominantly in the middle of the cell, but appr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050641
更新日期：1998-10-01 00:00:00
abstract：:RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacte...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100277
更新日期：2001-06-01 00:00:00
abstract：:Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00422282
更新日期：1988-01-01 00:00:00
abstract：:The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was de...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
abstract：:The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02085-6
更新日期：2020-10-19 00:00:00
abstract：:Type I signal peptidases are responsible for the proteolytic cleavage of the signal peptide of secreted proteins. In the gram-positive bacterium Streptomyces lividans, four adjacent genes (sipW, sipX, sipY and sipZ) were isolated encoding putative type I signal peptidases. In this work, the different sip genes were cl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100335
更新日期：2001-11-01 00:00:00
abstract：:In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00411361
更新日期：1979-08-06 00:00:00
abstract：:Cadmium is a widespread pollutant that has been associated with oxidative stress, but the mechanism behind this effect in prokaryotes is still unclear. In this work, we exposed two glutathione deficient mutants (DeltagshA and DeltagshB) and one respiration deficient mutant (DeltaubiE) to a sublethal concentration of c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0316-8
更新日期：2008-03-01 00:00:00
