Abstract：
:The presence of one periplasmic [NiFe] hydrogenase, one periplasmic [Fe] hydrogenase, and one cytoplasmic NADP-reducing hydrogenase has been previously established in Desulfovibrio fructosovorans. In the present work, marker-exchange mutagenesis was performed to determine the function of the tetrameric NADP-reducing hydrogenase encoded by the hndA, B, C, and D genes. The mutations performed were not lethal to the cells, although the H2-dependent NADP reduction was completely abolished. The double-mutated DM4 (DeltahynABC, DeltahndD) strain was still able to grow on hydrogen plus sulfate as the sole energy source. The growth may have occurred under these culture conditions because of the presence of the remaining [Fe] hydrogenase. The cells grew differently on various substrates depending on whether fructose, lactate, or pyruvate was used in the presence of sulfate. The (hnd mutant growth rates were 25-70% lower than those of the wild-type strain, although the molar growth yield remained unchanged. By contrast, mutants devoid of both [NiFe] hydrogenase and NADP-reducing hydrogenase had 24-38% lower growth yields and showed a corresponding drop in the growth rates. We concluded that each of the three hydrogenases may contribute to the energy supply in D. fructosovorans and that the loss of one enzyme might be compensated for by another. However, the loss of two hydrogenases affected the phosphorylation accompanying the metabolism of fructose, lactate, and pyruvate.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Malki S,De Luca G,Fardeau ML,Rousset M,Belaich JP,Dermoun Zdoi
10.1007/s002030050414subject
Has Abstractpub_date
1997-01-01 00:00:00pages
38-45issue
1eissn
0302-8933issn
1432-072Xjournal_volume
167pub_type
杂志文章abstract：:The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1222-8
更新日期：2016-09-01 00:00:00
abstract：:In this study, two quorum sensing (QS) system genes, las and rhI; N-3-oxo-dodecanoyl homoserine lactone (AHL; 3-O-C12-HSL); and QS-related virulence factors and correlation between them were assessed in 30 fish origin P. aeruginosa isolates. The detection of two QS system of the isolates, and eight gene regions consis...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02159-5
更新日期：2021-01-04 00:00:00
abstract：:Cell free extracts prepared from exponentially growing Escherichia coli and Bacillus cereus as well as from Bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with Bacillus ribonucleic acid. All three preparations synthesized glutam...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00455354
更新日期：1981-04-01 00:00:00
abstract：:The attachment of four species of bacteria to solid surfaces was investigated to determine whether the attachment of one species of bacterium could be influenced by the presence of other attaching or attached species. Three types of experiment were done: attachment of bacteria from suspensions containing two species (...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429648
更新日期：1987-06-01 00:00:00
abstract：:Gene probe hybridization was used to determine distribution and expression of co-metabolic genes at a contaminated site as it underwent in situ methanotrophic bioremediation of trichloroethylene (TCE). The bioremediation strategies tested included a series of air, air:methane, and air:methane:nutrient pulses of the te...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0445-8
更新日期：2009-03-01 00:00:00
abstract：:To overcome the extracellular salt stress, Methanohalophilus portucalensis FDF1(T) synthesizes the compatible solute betaine through the methylation of glycine, sarcosine, and N,N-dimethylglycine. S-adenosylmethionine (AdoMet) is the methyl donor. The enzyme sarcosine dimethylglycine N-methyltransferase (SDMT) of M. p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0501-z
更新日期：2009-10-01 00:00:00
abstract：:Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100350
更新日期：2001-12-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1051-6
更新日期：2015-03-01 00:00:00
abstract：:The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00381781
更新日期：1995-02-01 00:00:00
abstract：:The mitochondrial genome (mtDNA) of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae, with a total size of 24,673 bp, was one of the smallest known mtDNAs of Pezizomycotina. It contained the 14 typical genes coding for proteins related to oxidative phosphorylation, the two rRNA genes, a single intron...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0104-x
更新日期：2006-06-01 00:00:00
abstract：:An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050745
更新日期：1999-08-01 00:00:00
abstract：:Essential oils (EOs) are products of plant origin and include mixtures of different chemical compounds. These volatile substances have many interesting properties, including antifungal properties. Fungi may develop under field conditions on crops such as wheat or corn and are able to synthesize mycotoxins, which adver...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01673-5
更新日期：2019-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050465
更新日期：1997-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0499-2
更新日期：2009-09-01 00:00:00
abstract：:A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245249
更新日期：1992-01-01 00:00:00
abstract：:The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was de...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
abstract：:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombinati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409661
更新日期：1989-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1528-9
更新日期：2018-09-01 00:00:00
abstract：:The essential oil (EO) from the roots of Plectranthus barbatus Andr. (Syn. Coleus forskohlii Briq.) was evaluated for quorum sensing (QS) inhibitory activity. P. barbatus EO was screened for inhibition of QS regulated violacein production in Chromobacterium violaceum (ATCC 12472) wild-type strain. At inhibitory (6.25%...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02171-9
更新日期：2021-01-20 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100315
更新日期：2001-09-01 00:00:00
abstract：:The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0865-3
更新日期：2013-03-01 00:00:00
abstract：:A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated YXT, was isolated from wetland soil. Compared to strain YXT, Pedobacter yonginense HMD1002T had the highest 16S rRNA gene sequence identity (97.8%), and the remaining strains had the identities below 97%. Phylogenetic an...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1605-0
更新日期：2019-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416972
更新日期：1976-10-11 00:00:00
abstract：:RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacte...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100277
更新日期：2001-06-01 00:00:00
abstract：:Periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. Sequential colonization of a broad range of bacteria, including Fusobacterium nucleatum and Porphyromonas gingivalis, is an important phenomenon in this disease mode...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1223-7
更新日期：2016-08-01 00:00:00
abstract：:Given the serious threat of foodborne multidrug-resistant bacteria to animals and humans, finding an effective antibacterial compound has always been an important topic for scientists. Here, from the soil of Changbaishan, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus. Nr genome da...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02102-8
更新日期：2020-11-04 00:00:00
abstract：:The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
abstract：:Plasmid-borne hup-lacZ transcriptional fusion constructs were introduced into three separate mutant strains of Bradyrhizobium japonicum which express hydrogenase constitutively (Hupc strains SR470, SR473 and JH101) in both autotrophic and heterotrophic environments. The lacZ structural gene linked directly to the regu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00258144
更新日期：1993-01-01 00:00:00
abstract：:Exogenous addition of adenosine- and guanosine 5'-(di- and tri) phosphate 3'-diphosphate (pppApp, ppApp, pppGpp and ppGpp) at the concentration of 0.5 mM inhibits spore germination of Streptomyces galilaeus ATCC 31133. This reversible inhibitory effect appeared to be at the transcriptional level, and also depends on t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446889
更新日期：1980-04-01 00:00:00
abstract：:L-Alanine dehydrogenase was found in extracts of the antibiotic producer Streptomyces clavuligerus. The enzyme was induced by ammonia, and the level of induction was dependent on the extracellular concentraction. L-Alanine was the only amino acid able to induce alanine dehydrogenase. The enzyme was characterized from ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00403210
更新日期：1980-03-01 00:00:00
