Use of an acidophilic yeast strain to enable the growth of leaching bacteria on solid media.

Abstract:

:In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including organic acids. These properties allowed the yeast strain to enable and fasten the growth of iron and sulfur oxidizers on double-layer agar plate. The isolates were identified as Acidithiobacillus ferrooxidans FOX1, Leptospirillun ferriphilum BN, and Acidithiobacillus thiooxidans ZMB. These three leaching bacteria were inhibited by organic acids such as acetic and propionic acids; however, their activities were enhanced by Candida digboiensis NB under dissolved organic matter stress.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Ngom B,Liang Y,Liu Y,Yin H,Liu X

doi

10.1007/s00203-014-1051-6

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

339-46

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

197

pub_type

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