Abstract:
:In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity, and inhibition by diisopropyl fluorophosphate and phenylmethlysulfonylfluoride, the proteases were structurally dissimilar as revealed by (1) their amino acid compositions, (2) their inhibition by subtilisin inhibitor, (3) their immunological response to specific anti-Conidiobolus protease antibody, and (4) their tryptic peptide maps. Our results demonstrate that although they are functionally analogous, the Conidiobolus protease is structurally distinct from subtilisin Carlsberg. The Conidiobolus protease was also different from other bacterial and animal proteases (e.g. pronase, protease K, trypsin, and chymotrypsin) as evidenced by their lack of response to anti-Conidiobolus protease antibody in double diffusion and in neutralization assays. The Conidiobolus serine protease fails to obey the general rule that proteins with similar functions have similar primary sequences and, thus, are evolutionarily related. Our results strengthen the concept of convergent evolution for serine proteases and provide basis for research in evolutionary relationships among fungal, bacterial, and animal proteases.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Phadtare S,Rao M,Deshpande Vdoi
10.1007/BF01682989subject
Has Abstractpub_date
1996-12-01 00:00:00pages
414-7issue
6eissn
0302-8933issn
1432-072Xjournal_volume
166pub_type
杂志文章abstract::A new, phylogenetically distinct, dissimilatory, Fe(III)-reducing bacterium was isolated from surface sediment of a hydrocarbon-contaminated ditch. The isolate, designated strain PAL-1, was an obligately anaerobic, non-fermentative, motile, gram-negative vibrio. PAL-1 grew in a defined medium with acetate as electron ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050340
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doi:10.1007/BF00446777
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402302
更新日期:1978-05-30 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章,评审
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更新日期:2020-08-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0589-1
更新日期:2010-08-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01774-1
更新日期:2020-04-01 00:00:00
abstract::Previous work has shown that the mechanosensitive (MS) channel of large conductance (MscL) is essential for preventing lysis of Bacillus subtilis log phase cells upon a rapid, severe osmotic downshock. Growing cells of B. subtilis strains lacking MscL and one or more putative MS channel proteins of small conductance (...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0292-z
更新日期:2008-01-01 00:00:00
abstract::The coloration of cells of the cyanobacterium Synechococcus sp. PCC 7002 changed from normal blue-green to yellow-green when cells were grown at 15 degrees C in a medium containing nitrate as the sole nitrogen source. This change of coloration was similar to a general response to nutrient deprivation (chlorosis). For ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050535
更新日期:1998-01-01 00:00:00
abstract::A novel coccoid-shaped, hyperthermophilic, heterotrophic member of the archaea was isolated from a shallow marine hydrothermal system at Vulcano Island, Italy. The isolate grew between 56 and 90 degrees C with an optimum around 85 degrees C. The pH range for growth was 6.5 to 10.5, with an optimum around 9.0. Polysulf...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1431-9
更新日期:2018-01-01 00:00:00
abstract::An unique isotope labelling experiment was conducted whereby mixtures of sulfate and sulfite of different isotopic compositions were metabolized by Clostridium pasteurianum. The results showed during reduction of 1 mM SO3= plus 1 mM SO4=, essentially all evolved H2S arose from the sulfite whereas in the case of cellul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446644
更新日期:1976-09-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1237-1
更新日期:2016-10-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0444-0
更新日期:2002-08-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00436334
更新日期:1975-03-12 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0067-3
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journal_title:Archives of microbiology
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更新日期:2007-01-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447300
更新日期:1975-06-20 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1104-5
更新日期:2015-06-01 00:00:00
abstract::Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato (B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurre...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1350-9
更新日期:2017-07-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416970
更新日期:1976-10-11 00:00:00
abstract::A temperature-sensitive mutant of Salmonella typhimurium was isolated earlier after transposon mutagenesis with Tn10d Tet. The mutant D220 grows well at 28 degreesC but has a lower growth rate and forms filaments at 37 degreesC. Transposon-flanking fragments of mutant D220 DNA were cloned and sequenced. The transposon...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050607
更新日期:1998-06-01 00:00:00
abstract::Development of a transformation system in the n-alkane-assimilating diploid yeast Candida tropicalis requires an antibiotic resistance gene in order to establish a selectable marker. The resistance gene for hygromycin B has often been used as a selectable marker in yeast transformation. However, C. tropicalis harborin...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002039900125
更新日期:2000-03-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454856
更新日期:1976-07-01 00:00:00
abstract::The cold shock response in bacteria involves the expression of low-molecular weight cold shock proteins (CSPs) containing a nucleic acid-binding cold shock domain (CSD), which are known to destabilize secondary structures on mRNAs, facilitating translation at low temperatures. Caulobacter crescentus cspA and cspB are ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0602-8
更新日期:2010-09-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01981-1
更新日期:2020-12-01 00:00:00
abstract::Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period...
journal_title:Archives of microbiology
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doi:10.1007/s00203-019-01618-y
更新日期:2019-03-01 00:00:00
abstract::The present investigation deals with the optimization of cell growth rate of the candidate probiotic Pediococcus acidilactici in the presence of the specific prebiotic inulin. Three independent variables viz. concentration of inulin, concentration of glucose and pH have been selected for optimization study using respo...
journal_title:Archives of microbiology
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更新日期:2015-09-01 00:00:00