Abstract：
:A neutral protease (npr) (designated Bae16) toxic to nematodes was purified to homogeneity from the strain Bacillus nematocida. The purified protease showed a molecular mass of approximately 40 kDa and displayed optimal activity at 55 degrees C, pH 6.5. Bioassay experiments demonstrated that this purified protease could destroy the nematode cuticle and its hydrolytic substrates included gelatin and collagen. The gene encoding Bae16 was cloned, and the deduced amino acid sequence showed 94% sequence identity with npr gene from B. amyloliquefaciens, but had low similarity (13-43%) with the previously reported virulence serine proteases from fungi or bacteria, which reflected their differences. Recombinant mature Bae16 (rm-Bae16) was expressed in Escherichia coli BL21 using pET30 vector system, and its nematicidal activity confirmed that Bae16 could be involved in the infection process. Our present study revealed that the npr besides the known alkaline serine protease could serve as a potential virulence factor in the infection against nematodes, furthermore, the two proteases with different characteristics produced by the same strain co-ordinated efforts to kill nematodes. These data helped to understand the interaction between this bacterial pathogen and its host.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Niu Q,Huang X,Zhang L,Li Y,Li J,Yang J,Zhang Kdoi
10.1007/s00203-006-0112-xsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
439-48issue
6eissn
0302-8933issn
1432-072Xjournal_volume
185pub_type
杂志文章abstract：:Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100252
更新日期：2001-03-01 00:00:00
abstract：:Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1420-z
更新日期：2017-12-01 00:00:00
abstract：:Oxalobacter formigenes (O. formigenes) is a nonpathogenic, Gram-negative, obligate anaerobic bacterium that commonly inhabits the human gut and degrades oxalate as its major energy and carbon source. Results from a case-controlled study suggested that lack of O. formigenes colonization is a risk factor for recurrent c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1272-y
更新日期：2016-12-01 00:00:00
abstract：:We previously cloned the sigH gene encoding a stress-response sigma factor sigma(H) in Streptomyces coelicolor A3(2), located in an operon with the gene encoding proposed anti-sigma factor UshX. To clarify the in vivo function of sigma(H), a stable null mutant of sigH was prepared by homologous recombination. This mut...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0367-1
更新日期：2001-12-01 00:00:00
abstract：:The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050505
更新日期：1997-10-01 00:00:00
abstract：:Sulfolane (tetrahydrothiophene-1,1-dioxide) is used in the Sulfinol process for natural gas sweetening. At many sour-gas processing plants spills, landfills and leakage from unlined surface storage ponds have contaminated groundwaters with sulfolane. Due to its high water solubility and mobility in aquifers, sulfolane...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000184
更新日期：2000-07-01 00:00:00
abstract：:Pyruvate kinase (ATP: pyruvate phosphotransferase (EC 2.7.1.40) was partially purified from both autotrophically and heterotrophically grown Paracoccus denitrificans. The organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. The enzyme isolated from b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427847
更新日期：1977-10-24 00:00:00
abstract：:The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA syn...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446529
更新日期：1975-12-31 00:00:00
abstract：:Fifty isolates belonging to the six validly described species of the genus Thermus (T. aquaticus, T. filiformis, T. thermophilus, T. scotoductus, T. brockianus, and T. oshimai) isolated from hot springs of different geographical areas were compared using macrorestriction analysis of genomic DNA and ribotyping. With th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050474
更新日期：1997-08-01 00:00:00
abstract：:L-(+)-lactate dehydrogenase (LDH) from Staphylococcus epidermidis ATCC 14990 was purified by affinity chromatography. The purified enzyme was specifically activated by fructose-1,6-diphosphate (FDP). The concentration of FDP required for 50% maximal activity was about 0.15 mM. The enzyme activity was inhibited by aden...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447150
更新日期：1975-11-07 00:00:00
abstract：:The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00381781
更新日期：1995-02-01 00:00:00
abstract：:The spectroscopic properties of stigma inside green and dark-grown cells and of isolated stigma globules have been studied by means of a microspectrophotometer build in the Laboratory. On the base of these results and of the analysis of the absorption spectra of a stigma suspension, cell suspension and the cell methan...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446551
更新日期：1976-12-01 00:00:00
abstract：:Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhib...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410896
更新日期：1984-08-01 00:00:00
abstract：:Coenzyme F420 is a 8-hydroxy-5-deazaflavin present in methanogenic bacteria. We have investigated whether the pyrimidine ring of the deazaflavin originates from guanine as in flavin biosynthesis, in which the pyrimidine ring of guanine is conserved. For this purpose the incorporation of [2-14C]guanine and of [8-14C]gu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410735
更新日期：1984-04-01 00:00:00
abstract：:Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02133-1
更新日期：2020-12-15 00:00:00
abstract：:Microorganisms have evolved themselves to thrive under various extreme environmental conditions such as extremely high or low temperature, alkalinity, and salinity. These microorganisms adapted several metabolic processes to survive and reproduce efficiently under such extreme environments. As the major proportion of ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-018-1602-3
更新日期：2019-01-01 00:00:00
abstract：:Upland rice is an ecotype crop resulting from the long-term domestication and evolution of rice in dry land without a water layer. Generally, the stems and leaves are thick and luxuriant, while the leaves also typically broad and light. The root system is developed with abundant root hair, and the osmotic pressure of ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02058-9
更新日期：2020-09-29 00:00:00
abstract：:Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00444661
更新日期：1989-01-01 00:00:00
abstract：:A thermostable homodimeric isocitrate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was purified and characterized. The mol. mass of the isocitrate dehydrogenase subunit was 42 kDa as determined by SDS-PAGE. Following separation by SDS-PAGE, A. fulgidus isocitrate dehydrogen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050516
更新日期：1997-11-01 00:00:00
abstract：:Inorganic polyphosphate (iPoP)-linear chains of up to hundreds of phosphate residues-is ubiquitous in nature and appears to be involved in many different cellular processes. In Saccharomyces cerevisiae, iPoP has been detected in high concentrations, especially after transfer of phosphate-deprived cells to a high-phosp...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0031-2
更新日期：2005-11-01 00:00:00
abstract：:The occurrence of emodin, erythroglaucin, physcion, physcion-9-anthrone, questin, catenarin, and catenarin-8-methyl ether in different species of the Aspergillus glaucus group (genus Eurotium) was investigated. So far catenarin-8-methyl ether (1, 4, 6-trihydroxy-8-methoxy-3-methylanthraquinone) has not been described ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409924
更新日期：1980-07-01 00:00:00
abstract：:Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00412799
更新日期：1986-01-01 00:00:00
abstract：:The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406170
更新日期：1980-12-01 00:00:00
abstract：:P1B-type ATPases are involved in heavy metal transport across the plasma membrane. Some Mycobacterium tuberculosis P-type ATPases are induced during infection, suggesting that this type of transporter could play a critical role in mycobacterial survival. To date, the ion specificity of M. tuberculosis heavy metal-tran...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1465-z
更新日期：2018-04-01 00:00:00
abstract：:Present study attempts in revealing taxonomic and functional diversity of microorganism from petroleum muck using metagenomics approach. Using Ion Torrent Personal Genome Machine, total of 249 Mb raw data were obtained which was analysed using MG-RAST platform. The taxonomic analysis revealed predominance of Proteobac...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0992-0
更新日期：2014-08-01 00:00:00
abstract：:Chemical mutagenesis of the nucleotide-producing strain Corynebacterium ammoniagenes ATCC 6872 with N-methyl-N-nitro-N-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. A rapid assay for the allosterically regulated Mn-ribonucleotide reductase (RRase) was developed with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050450
更新日期：1997-05-01 00:00:00
abstract：:The upstream region of the isocitrate lyase gene (UPR-ICL, 1530bp) of an n-alkane-utilizable yeast, Candida tropicalis, induced gene expression in another yeast, Saccharomyces cerevisiae, when the yeasts were grown on acetate. Surprisingly, UPR-ICL displayed the same regulatory function in the bacterium Escherichia co...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00404204
更新日期：1995-05-01 00:00:00
abstract：:NAD-linked lactate dehydrogenases specific for the D- and L-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied. The enzyme has a molecular weight of ca. 115000-120000...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447300
更新日期：1975-06-20 00:00:00
abstract：:To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000236
更新日期：2001-01-01 00:00:00
abstract：:The natural role of antibiotics in the ecology of Streptomyces is debated and still largely unknown. The predatory myxobacteria and many other genera of prokaryotic epibiotic and wolfpack predators across different taxa possess secondary metabolites with antimicrobial action, and these compounds have a role in predati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0961-7
更新日期：2014-04-01 00:00:00
