Studies on the biosynthesis of coenzyme F420 in methanogenic bacteria.

Abstract:

:Coenzyme F420 is a 8-hydroxy-5-deazaflavin present in methanogenic bacteria. We have investigated whether the pyrimidine ring of the deazaflavin originates from guanine as in flavin biosynthesis, in which the pyrimidine ring of guanine is conserved. For this purpose the incorporation of [2-14C]guanine and of [8-14C]guanine into F420 by growing cultures of Methanobacterium thermoautotrophicum was studied. Only in the case of [2-14C]guanine did F420 become labeled. The specific radioactivity of the deazaflavin and of guanine isolated from nucleic acids of [2-14C]guanine grown cells were identical. This finding suggests that the pyrimidine ring of the deazaflavin and of flavins are synthesized by the same pathway. F420 did not become labeled when M. thermoautotrophicum was grown in the presence of methyl-[14C]methionine, [U-14C]phenylalanine or [U-14C]tyrosine. This excludes that C-5 of the deazaflavin is derived from the methyl group of methionine and that the benzene ring comes from phenylalanine or tyrosine.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Jaenchen R,Schönheit P,Thauer RK

doi

10.1007/BF00410735

subject

Has Abstract

pub_date

1984-04-01 00:00:00

pages

362-5

issue

4

eissn

0302-8933

issn

1432-072X

journal_volume

137

pub_type

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