当前位置： SCI文献检索 > ARCHIVES OF MICROBIOLOGY期刊下所有文献 > Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA.

Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA.

Abstract：

:Clostridium aminobutyricum ferments 4-aminobutyrate via succinic semialdehyde, 4-hydroxybutyrate, 4-hydroxybutyryl-CoA and crotonyl-CoA to acetate and butyrate. The genes coding for the enzymes that catalyse the interconversion of these intermediates are arranged in the order abfD (4-hydroxybutyryl-CoA dehydratase), abfT (4-hydroxybutyrate CoA-transferase), and abfH (NAD-dependent 4-hydroxybutyrate dehydrogenase). The genes abfD and abfT were cloned, sequenced and expressed as active enzymes in Escherichia coli. Hence the insertion of the [4Fe-4S]clusters and FAD into the dehydratase required no additional specific protein from C. aminobutyricum. The amino acid sequences of the dehydratase and the CoA-transferase revealed close relationships to proteins deduced from the genomes of Clostridium difficile, Porphyromonas gingivalis and Archaeoglobus fulgidus. In addition the N-terminal part of the dehydratase is related to those of a family of FAD-containing mono-oxygenases from bacteria. The putative assignment in the databank of Cat2 (OrfZ) from Clostridium kluyveri as 4-hydroxybutyrate CoA-transferase, which is thought to be involved in the reductive pathway from succinate to butyrate, was confirmed by sequence comparison with AbfT (57% identity). Furthermore, an acetyl-CoA:4-hydroxybutyrate CoA-transferase activity could be detected in cell-free extracts of C. kluyveri. In contrast to glutaconate CoA-transferase from Acidaminococcus fermentans, mutation studies suggested that the glutamate residue of the motive EXG, which is conserved in many homologues of AbfT, does not form a CoA-ester during catalysis.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Gerhardt A,Cinkaya I,Linder D,Huisman G,Buckel W

doi

10.1007/s002030000195

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

189-99

issue

3

eissn

0302-8933

issn

1432-072X

journal_volume

174

pub_type

杂志文章

相关文献

ARCHIVES OF MICROBIOLOGY文献大全
  • Acetate thiokinase and the assimilation of acetate in methanobacterium thermoautotrophicum.

    abstract：:Methanobacterium thermoautotrophicum growing on H2 plus CO2 as sole carbon and energy source was found to contain acetate thiokinase (Acetyl CoA synthetase; EC 6.2.1.1); Acetate + ATP + CoA leads to Acetyl CoA + AMP + PPi. The apparent Km value for acetate was 40 microM. Acetate kinase (EC 2.7.2.1) and phosphotransace...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00406167

    authors： Oberlies G,Fuchs G,Thauer RK

    更新日期：1980-12-01 00:00:00

  • Xylose and some non-sugar carbon sources cause catabolite repression in Saccharomyces cerevisiae.

    abstract：:Glucose and other sugars, such as galactose or maltose, are able to cause carbon catabolite repression in Saccharomyces cerevisiae. Although glycolytic intermediates have been suggested as signal for repression, no evidence for such a control mechanism is available. The establishment of a correlation between levels of...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-003-0593-9

    authors： Belinchón MM,Gancedo JM

    更新日期：2003-10-01 00:00:00

  • Transport of branched-chain amino acids in Corynebacterium glutamicum.

    abstract：:The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 micro...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00413136

    authors： Ebbighausen H,Weil B,Krämer R

    更新日期：1989-01-01 00:00:00

  • Aldolases of the lactic acid bacteria. Demonstration of immunological relationships among eight genera of Gram positive bacteria using an anti-pediococcal aldolase serum.

    abstract：:Reciprocal qualitative and quantitative immunological experiments employing an anti-Pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the Streptococcus faecalis fructose diphosphate aldolase. The ext...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00416976

    authors： London J,Chace NM

    更新日期：1976-10-11 00:00:00

  • A mechanistic study on SMOB-ADP1: an NADH:flavin oxidoreductase of the two-component styrene monooxygenase of Acinetobacter baylyi ADP1.

    abstract：:Two styrene monooxygenase types, StyA/StyB and StyA1/StyA2B, have been described each consisting of an epoxidase and a reductase. A gene fusion which led to the chimeric reductase StyA2B and the occurrence in different phyla are major differences. Identification of SMOA/SMOB-ADP1 of Acinetobacter baylyi ADP1 may enlig...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-014-1022-y

    authors： Gröning JA,Kaschabek SR,Schlömann M,Tischler D

    更新日期：2014-12-01 00:00:00

  • Localization and activities of nitrogenase, glutamine synthetase and glutamate synthase in Azotobacter vinelandii grown in oxygen-controlled continuous culture.

    abstract：:Azotobacter vinelandii was grown in oxygen-controlled continuous cultures under conditions of dinitrogen fixation. Different oxygen concentrations were adjusted with air. Cell-free extracts were employed to study the oxygen dependency of the intracellular distribution and activity of the following enzymes: nitrogenase...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00415614

    authors： Röckel D,Hernando JJ,Vakalopoulou E,Post E,Oelze J

    更新日期：1983-10-01 00:00:00

  • Identification and molecular characterization of twin-arginine translocation system (Tat) in Xanthomonas oryzae pv. oryzae strain PXO99.

    abstract：:Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, one of the most widespread and destructive bacterial diseases in rice. This study identified and characterized the contribution of the twin-arginine translocation (Tat) pathway to motility, chemotaxis, extracellular polysaccharide (EPS) production and virulen...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-008-0440-0

    authors： Chen L,Hu B,Qian G,Wang C,Yang W,Han Z,Liu F

    更新日期：2009-02-01 00:00:00

  • Association of a new type of gliding, filamentous, purple phototrophic bacterium inside bundles of Microcoleus chthonoplastes in hypersaline cyanobacterial mats.

    abstract：:An unidentified filamentous purple bacterium, probably belonging to a new genus or even a new family, is found in close association with the filamentous, mat-forming cyanobacterium Microcoleus chthonoplastes in a hypersaline pond at Guerrero Negro, Baja California Sur, Mexico, and in Solar Lake, Sinai, Egypt. This or...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00463477

    authors： D'Amelio ED,Cohen Y,Des Marais DJ

    更新日期：1987-01-01 00:00:00

  • Regulation of citB expression in Bacillus subtilis: integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system.

    abstract：:The tricarboxylic acid (TCA) cycle is one of the major routes of carbon catabolism in Bacillus subtilis. The syntheses of the enzymes performing the initial reactions of the cycle, citrate synthase, and aconitase, are synergistically repressed by rapidly metabolizable carbon sources and glutamine. This regulation invo...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-005-0078-0

    authors： Blencke HM,Reif I,Commichau FM,Detsch C,Wacker I,Ludwig H,Stülke J

    更新日期：2006-03-01 00:00:00

  • Relatedness of the flagellins from methanogens.

    abstract：:Purified flagellar filaments isolated from six methanogens were composed of multiple flagellins. Two flagellins were present in Methanococcus deltae (Mr = 34,000 and 32,000), Methanoculleus marisnigri (Mr = 31,000 and 25,500) and Methanococcus jannaschii (Mr = 31,000 and 27,500), three in Methanothermus fervidus (Mr =...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00276766

    authors： Kalmokoff ML,Koval SF,Jarrell KF

    更新日期：1992-01-01 00:00:00

  • Phosphofructokinase activities within the order spirochaetales and the characterisation of the pyrophosphate-dependent phosphofructokinase from spirochaeta thermophila

    abstract：:The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermo...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s002030050777

    authors： Ronimus RS,Morgan HW,Ding YR

    更新日期：1999-12-01 00:00:00

  • Regulation of the central metabolism in relation to citric acid production in Saccharomycopsis lipolytica.

    abstract：:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00428588

    authors： Marchal R,Vandecasteele JP,Metche M

    更新日期：1977-05-13 00:00:00

  • The molecular regulation of the reductive pentose phosphate pathway in Proteobacteria and Cyanobacteria.

    abstract：:In phototrophic and chemoautotrophic proteobacteria, genes encoding enzymes of the Calvin-Benson-Bassham pathway of CO2 fixation are often found in clusters that are transcribed from a single promoter under control of the LysR-type transcriptional activator, CbbR. Mutations affecting CbbR prevent induction of cbb gene...

    journal_title：Archives of microbiology

    pub_type： 杂志文章,评审

    doi：10.1007/s002030050369

    authors： Gibson JL,Tabita FR

    更新日期：1996-09-01 00:00:00

  • In situ growth of the novel SM1 euryarchaeon from a string-of-pearls-like microbial community in its cold biotope, its physical separation and insights into its structure and physiology.

    abstract：:Recently, a unique archaeal/bacterial community that grows in a macroscopically visible string-of-pearls-like structure in cold (~10 degrees C), sulfurous marsh water was discovered. Here, a new technique is described that allows the fast and reliable growth of these string-of-pearls-like microbial communities in larg...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-003-0580-1

    authors： Moissl C,Rudolph C,Rachel R,Koch M,Huber R

    更新日期：2003-09-01 00:00:00

  • Intracellular localization of the particulate methane monooxygenase and methanol dehydrogenase in Methylomicrobium album BG8.

    abstract：:The methanotrophic bacterium Methylomicrobium album BG8 uses methane as a sole source of carbon and energy. This bacterium forms an extensive intracytoplasmic membrane. The first enzymes of the methane oxidation pathway are the membrane-bound particulate methane monooxygenase and the periplasmic methanol dehydrogenase...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-002-0426-2

    authors： Brantner CA,Remsen CC,Owen HA,Buchholz LA,Perille Collins ML

    更新日期：2002-07-01 00:00:00

  • Genome-wide analysis of DNA methylation in subcultured Cordyceps militaris.

    abstract：:The entomopathogenic mushroom Cordyceps militaris is a storehouse of various medicinal compounds and pharmacological effects. However, the high frequency of strain degeneration during subculture and preservation severely limits the large-scale production of C. militaris. DNA methylation is an important epigenomic modi...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-019-01621-3

    authors： Xin X,Yin J,Zhang B,Li Z,Zhao S,Gui Z

    更新日期：2019-04-01 00:00:00

  • Some properties of a Klebsiella pneumoniae ammonium transport negative mutant (Amt-).

    abstract：:The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH4+ intracellularly. When growing on nitrogen sources other than NH4+, the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of ...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00402008

    authors： Castorph H,Kleiner D

    更新日期：1984-10-01 00:00:00

  • Pedobacter paludis sp. nov., isolated from wetland soil.

    abstract：:A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated YXT, was isolated from wetland soil. Compared to strain YXT, Pedobacter yonginense HMD1002T had the highest 16S rRNA gene sequence identity (97.8%), and the remaining strains had the identities below 97%. Phylogenetic an...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-018-1605-0

    authors： Zhang Y,Li J,Wang J,Wang G

    更新日期：2019-04-01 00:00:00

  • Roles of the glutathione- and thioredoxin-dependent systems in the Escherichia coli responses to ciprofloxacin and ampicillin.

    abstract：:Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-016-1247-z

    authors： Smirnova G,Muzyka N,Lepekhina E,Oktyabrsky O

    更新日期：2016-11-01 00:00:00

  • The use of stable sulfur isotope labelling to elucidate sulfur metabolism by Clostridium pasteurianum.

    abstract：:An unique isotope labelling experiment was conducted whereby mixtures of sulfate and sulfite of different isotopic compositions were metabolized by Clostridium pasteurianum. The results showed during reduction of 1 mM SO3= plus 1 mM SO4=, essentially all evolved H2S arose from the sulfite whereas in the case of cellul...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00446644

    authors： McCready RG,Laishley EJ,Krouse HR

    更新日期：1976-09-01 00:00:00

  • Sophorose induction of an intracellular b-glucosidase in Trichoderma.

    abstract：:The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00425807

    authors： Loewenberg JR

    更新日期：1984-01-01 00:00:00

  • Insights from genome of Clostridium butyricum INCQS635 reveal mechanisms to convert complex sugars for biofuel production.

    abstract：:Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based o...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-015-1166-4

    authors： Bruce T,Leite FG,Miranda M,Thompson CC,Pereira N Jr,Faber M,Thompson FL

    更新日期：2016-03-01 00:00:00

  • Amelioration effect of chromium-tolerant bacteria on growth, physiological properties and chromium mobilization in chickpea (Cicer arietinum) under chromium stress.

    abstract：:In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-019-01801-1

    authors： Shreya D,Jinal HN,Kartik VP,Amaresan N

    更新日期：2020-05-01 00:00:00

  • Identification of the mycothiol synthase gene (mshD) encoding the acetyltransferase producing mycothiol in actinomycetes.

    abstract：:Mycothiol is the predominant thiol in most actinomycetes, including Mycobacterium tuberculosis, and appears to play a role analogous to glutathione, which is not found in these bacteria. The enzymes involved in mycothiol biosynthesis are of interest as potential targets for new drugs directed against tuberculosis. In ...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-002-0462-y

    authors： Koledin T,Newton GL,Fahey RC

    更新日期：2002-11-01 00:00:00

  • The effect of gyrase inhibitors and cyclic AMP on induction and glucose repression of the 6-hydroxy-nicotine oxidases in Arthrobacter oxidans.

    abstract：:The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00521289

    authors： Brandsch R,Decker K

    更新日期：1982-12-03 00:00:00

  • Terraforming: synthetic biology's final frontier.

    abstract：:Synthetic biology, the design and synthesis of synthetic biological systems from DNA to whole cells, has provided us with the ultimate tools for space exploration and colonisation. Herein, we explore some of the most significant advances and future prospects in the field of synthetic biology, in the context of astrobi...

    journal_title：Archives of microbiology

    pub_type： 信件

    doi：10.1007/s00203-019-01651-x

    authors： Sleator RD,Smith N

    更新日期：2019-08-01 00:00:00

  • Effect of adenosine-5'-triphosphate-3'-diphosphate and related nucleoside polyphosphates on the spore germination of Streptomyces galilaeus.

    abstract：:Exogenous addition of adenosine- and guanosine 5'-(di- and tri) phosphate 3'-diphosphate (pppApp, ppApp, pppGpp and ppGpp) at the concentration of 0.5 mM inhibits spore germination of Streptomyces galilaeus ATCC 31133. This reversible inhibitory effect appeared to be at the transcriptional level, and also depends on t...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00446889

    authors： Hamagishi Y,Tone H,Oki T,Inui T

    更新日期：1980-04-01 00:00:00

  • Fatty acid, polar lipid and wall amino acid composition of Gardnerella vaginalis.

    abstract：:Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00425410

    authors： O'Donnell AG,Minnikin DE,Goodfellow M,Piot P

    更新日期：1984-05-01 00:00:00

  • Heterogeneity of lipoteichoic acid detected by anion exchange chromatography.

    abstract：:A complex polydispersity became apparent when the poly(glycerophosphate) lipoteichoic acid of Enterococcus faecalis was chromatographed on DEAE-Sephadex. The chain length varied between 13 and 33 glycerophosphate residues per lipid anchor. In parallel, the extent of chain glycosylation increased from 0.2 to 0.4 digluc...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/BF00249103

    authors： Leopold K,Fischer W

    更新日期：1992-01-01 00:00:00

  • Increased mRNA expression of interferon-induced Mx1 and immunomodulation following oral administration of IFN-alpha2b-transformed B. longum to mice.

    abstract：:We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...

    journal_title：Archives of microbiology

    pub_type： 杂志文章

    doi：10.1007/s00203-010-0589-1

    authors： Yu Z,Zeng Z,Huang Z,Lian J,Yang J,Deng Q,Zeng W

    更新日期：2010-08-01 00:00:00