Abstract：
:DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus urease with the amino acid sequence of jack bean urease an overall portion of 56% identical residues was found. For S. xylosus urease a subunit structure of (alpha beta gamma)4 was proposed, based on the comparison of the deduced amino acid content of the enzyme subunits with the total amino acid content of the purified enzyme. The staphylococcal enzyme contained no cysteine, as deduced from DNA sequence and confirmed by the determination of the total amino acid content in the purified enzyme. Instead of cysteine, known to be catalytically essential in the plant enzyme, and conserved among all bacterial ureases analyzed so far, threonine was found in S. xylosus. This amino acid-exchange was located within a highly conserved domain of 17 amino acids, supposed to be part of the active site. Sequence analysis of the respective region of Staphylococcus saprophyticus urease showed that it also contains threonine instead of cysteine. In contrast to jack bean urease S. xylosus urease was not affected by the SH-group inhibitor dipyridyl disulfide but was completely inhibited by the serine protease inhibitor phenylmethanesulfonyl fluoride. The presented results indicate that in these staphylococcal strains urea hydrolysis might function in a manner similar to the peptide bond cleavage by chymotrypsin.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Jose J,Schäfer UK,Kaltwasser Hdoi
10.1007/BF00288947subject
Has Abstractpub_date
1994-01-01 00:00:00pages
384-92issue
5eissn
0302-8933issn
1432-072Xjournal_volume
161pub_type
杂志文章abstract：:A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447134
更新日期：1975-10-27 00:00:00
abstract：:After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492022
更新日期：1977-06-20 00:00:00
abstract：:It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has open...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-011-0733-6
更新日期：2012-01-01 00:00:00
abstract：:Video-enhanced light microscopy of the apical and subapical regions of growing hyphae of several fungal species revealed the existence of momentary synchronized motions of subcellular organelles. First discovered in a temperature-sensitive morphological mutant (ramosa-1) of Aspergillus niger, these seemingly spontaneo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0771-z
更新日期：2005-05-01 00:00:00
abstract：:Chemical mutagenesis of the nucleotide-producing strain Corynebacterium ammoniagenes ATCC 6872 with N-methyl-N-nitro-N-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. A rapid assay for the allosterically regulated Mn-ribonucleotide reductase (RRase) was developed with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050450
更新日期：1997-05-01 00:00:00
abstract：:The prpC and prkC genes encode two proteins with opposing activities, eukaryotic-like protein phosphatase and protein kinase involved in the sporulation and biofilm formation processes. The prpC gene overlaps 3 bp of the prkC gene; prkC is followed by the essential gene, yloQ. The organisation of the prpC, prkC and yl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0015-2
更新日期：2005-09-01 00:00:00
abstract：:The site of integration of phage M mu d (Ap lac) in mutant M9s which leads to deficiency of formic dehydrogenase (benzylviologen-linked) activity was determined. It was shown that the phage had inserted into the gene for the seleno-polypeptide of the enzyme (80 kd) leading to the formation of a truncated peptide (60 k...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428850
更新日期：1985-05-01 00:00:00
abstract：:An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050745
更新日期：1999-08-01 00:00:00
abstract：:L-Alanine dehydrogenase was found in extracts of the antibiotic producer Streptomyces clavuligerus. The enzyme was induced by ammonia, and the level of induction was dependent on the extracellular concentraction. L-Alanine was the only amino acid able to induce alanine dehydrogenase. The enzyme was characterized from ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00403210
更新日期：1980-03-01 00:00:00
abstract：:Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02003-w
更新日期：2021-01-01 00:00:00
abstract：:The symbiotic bacteria Bradyrhizobium japonicum and Sinorhizobium meliloti, and the purple photosynthetic bacteria Rhodobacter capsulatus, Rhodovulum sulfidophilum, Roseobacter denitrificans and Rhodobacter sphaeroides possess homologous two-component regulatory systems, namely RegSR, ActSR, RegBA and PrrBA. The respe...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000207
更新日期：2000-11-01 00:00:00
abstract：:The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleyl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00276301
更新日期：1992-01-01 00:00:00
abstract：:Nitrosomonas europaea, a Gram-negative obligate chemolithoautotroph, participates in global nitrogen cycling by carrying out nitrification and derives energy for growth through oxidation of ammonia. In this work, the physiological, proteomic, and transcriptional responses of N. europaea to zinc stress were studied. Th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0341-7
更新日期：2008-06-01 00:00:00
abstract：:To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0406-2
更新日期：2008-11-01 00:00:00
abstract：:The anticandidal potential of Geraniol (Ger) against Candida albicans has already been established. The present study reveals deeper insights into the mechanisms of action of Ger. We observed that the repertoire of antifungal activity was not only limited to C. albicans and its clinical isolates but also against non-a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1205-9
更新日期：2016-07-01 00:00:00
abstract：:The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01833-y
更新日期：2020-07-01 00:00:00
abstract：:BvgAS is a two-component system of Bordetella pertussis involved in the reciprocal regulation of the virulence genes and the flagellar biosynthesis. In this study, we found that expression of bvgAS in Escherichia coli also results in reduced motility. The repression was relieved by the addition of known chemical modul...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050688
更新日期：1999-01-01 00:00:00
abstract：:Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, one of the most widespread and destructive bacterial diseases in rice. This study identified and characterized the contribution of the twin-arginine translocation (Tat) pathway to motility, chemotaxis, extracellular polysaccharide (EPS) production and virulen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0440-0
更新日期：2009-02-01 00:00:00
abstract：:Pseudomonas putida CSV86 utilizes benzyl alcohol via catechol and methylnaphthalenes through detoxification pathway via hydroxymethylnaphthalenes and naphthaldehydes. Based on metabolic studies, benzyl alcohol dehydrogenase (BADH) and benzaldehyde dehydrogenase (BZDH) were hypothesized to be involved in the detoxifica...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0697-6
更新日期：2011-08-01 00:00:00
abstract：:Only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. Little is known about the dietary and physiological factors determining this ecological feature. In this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0747-4
更新日期：2005-01-01 00:00:00
abstract：:To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0582-8
更新日期：2010-07-01 00:00:00
abstract：:Purple sulfur bacterium Thiocapsa roseopersicina strain BBS requiring vitamin B12 may grow in the dark in media containing no other organic compounds. Under such conditions the cells oxidize sulfide and thiosulfate with the use of O2 and assimilate carbon dioxide. After 10--30s assimilation of NaH14CO3 about 60% of ra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454854
更新日期：1976-07-01 00:00:00
abstract：:During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea g...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01941-9
更新日期：2020-10-01 00:00:00
abstract：:Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050737
更新日期：1999-07-01 00:00:00
abstract：:Glyoxalase I is a ubiquitous enzyme that detoxifies methylglyoxal, which is derived from glycolysis but inhibits the growth of cells from microorganisms to mammals. Here, the structural gene for glyoxalase I ( glo1(+)) from the fission yeast Schizosaccharomyces pombe was identified. Disruption of glo1(+) enhanced susc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0666-4
更新日期：2004-05-01 00:00:00
abstract：:Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0686-0
更新日期：2004-10-01 00:00:00
abstract：:The chb operon of Escherichia coli is involved in the utilization of chitooligosaccharides. While acquisition of two classes of mutations leading to altered regulation of the chb operon is necessary to confer the ability to utilize the glucose disaccharide cellobiose to wild-type strains of E. coli, in the closely rel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1299-0
更新日期：2017-03-01 00:00:00
abstract：:ModE protein, a molybdate sensor/regulator, controls the transcription of genes coding for molybdate uptake (mod), molybdopterin synthesis (moa), molybdoenzymes nitrate reductase (nap) and dimethylsulfoxide reductase (dms), as well as fermentative dihydrogen production (fdhF and hyc) and respiratory nitrate reductase ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0039-7
更新日期：2005-12-01 00:00:00
abstract：:Expression of the gene encoding ribosome modulation factor (RMF), as measured using an rmf-lacZ gene fusion, increased with decreasing pH in exponential phase cultures of Escherichia coli. Expression was inversely proportional to the growth rate and independent of the acidifying agent used and it was concluded that ex...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0025-0
更新日期：2005-10-01 00:00:00
abstract：:Xanthan-deficient mutants of Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, were generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, GumD. Mutants of gumD did not produce xanthan and remained pathogenic in citrus pl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0227-8
更新日期：2007-08-01 00:00:00
