Abstract:
:To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosphate (PP) pathway and Entner Doudoroff (ED) pathway were up-regulated in the cra mutant. The glucose uptake rate and the acetate production rate were increased with less acetate consumption for the cra mutant. To identify the genes controlled by Cra protein, the Cra recognition weight matrix from foot-printing data was developed and used to scan the whole genome. Several new Cra-binding sites were found, and some of the result was consistent with the DNA microarray data. The ED pathway was active in the cra mutant; we constructed cra.edd double genes knockout mutant to block this pathway, where the acetate overflowed due to the down-regulation of aceA,B and icd gene expressions. Then we further constructed cra.edd.iclR triple genes knockout mutant to direct the carbon flow through the glyoxylate pathway. The cra.edd.iclR mutant showed the least acetate production, resulting in the highest cell yield together with the activation of the glycolysis pathway, but the glucose consumption rate could not be improved.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Sarkar D,Siddiquee KA,Araúzo-Bravo MJ,Oba T,Shimizu Kdoi
10.1007/s00203-008-0406-2subject
Has Abstractpub_date
2008-11-01 00:00:00pages
559-71issue
5eissn
0302-8933issn
1432-072Xjournal_volume
190pub_type
杂志文章abstract::Peptidoglycan of Corynebacterium species Co 112 (DSM 20606) exhibits an unknown amino acid. The amino acid was isolated from cell wall hydrolysates and identified as threo-beta-hydroxyornithine. This amino acid is found in the interpeptide bridge of the peptidoglycan of Corynebacterium sp. Co 112. The primary structur...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00407766
更新日期:1983-06-01 00:00:00
abstract::A novel coccoid-shaped, hyperthermophilic, heterotrophic member of the archaea was isolated from a shallow marine hydrothermal system at Vulcano Island, Italy. The isolate grew between 56 and 90 degrees C with an optimum around 85 degrees C. The pH range for growth was 6.5 to 10.5, with an optimum around 9.0. Polysulf...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1247-z
更新日期:2016-11-01 00:00:00
abstract::The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245305
更新日期:1993-01-01 00:00:00
abstract::To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000236
更新日期:2001-01-01 00:00:00
abstract::Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and f...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030050713
更新日期:1999-04-01 00:00:00
abstract::VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1069-9
更新日期:2015-04-01 00:00:00
abstract::The tricarboxylic acid (TCA) cycle is one of the major routes of carbon catabolism in Bacillus subtilis. The syntheses of the enzymes performing the initial reactions of the cycle, citrate synthase, and aconitase, are synergistically repressed by rapidly metabolizable carbon sources and glutamine. This regulation invo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0078-0
更新日期:2006-03-01 00:00:00
abstract::The association of nucleoside triphosphate molecules and calcium ions with purified particles of mycobacteriophage 13 has been documented. The content of nucleoside triphosphate has been determined to be 118 molecules per phage particle by equilibrium dialysis against labelled ATP or 148 molecules per phage particle b...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00527071
更新日期:1981-09-01 00:00:00
abstract::Plasmid pJP4 encoding the ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (Tfd+) was transferred by conjugation from Escherichia coli JMP397 to various lithoautotrophic strains of Alcaligenes eutrophus and to the autotrophic bacterium Pseudomonas oxalaticus. The herbicide-degrading function of the plas...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00407938
更新日期:1983-02-01 00:00:00
abstract::Exoglucanase (exo-1,3-beta-D-glucan glycohydrolase, EC 3.2.1.56) activity secreted by Saccharomyces cerevisiae into the culture medium was separated by ion exchange chromatography into two glycoprotein isoenzymes which contributed 10% (exoglucanase I) and 90% (exoglucanase II) towards the total activity. Analysis of t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416596
更新日期:1989-01-01 00:00:00
abstract::Bioleaching is a promising process for 350 million tons Jinchuan low-grade pentlandite. But, Jinchuan pentlandite has lots of magnesium and high concentration of Mg2+ is harmful to bioleaching microorganisms. Thus, finding a way to improve the adaption of microorganisms to Mg2+ is a key for bioleaching. In the study, ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-018-1503-5
更新日期:2018-09-01 00:00:00
abstract::A novel actinomycete, strain PA1-10T, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aer...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01717-w
更新日期:2020-01-01 00:00:00
abstract::Succinate:quinone reductase is a membrane-bound enzyme of the citric acid cycle and the respiratory chain. Carboxin is a potent inhibitor of the enzyme of certain organisms. The bacterium Paracoccus denitrificans was found to be sensitive to carboxin in vivo, and mutants that grow in the presence of 3'-methyl carboxin...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050611
更新日期:1998-07-01 00:00:00
abstract::The efficiency of hydrolysis of fungal (Fusarium spp.) cell wall and rye root cell wall by crude enzymatic complexes from (42-day-old) cultures of three F. culmorum isolates, a plant growth-promoting rhizosphere isolate (PGPF) DEMFc2, a deleterious rhizosphere isolate (DRMO) DEMFc5, and a pathogenic isolate DEMFc37, a...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0803-4
更新日期:2012-08-01 00:00:00
abstract::The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five uni...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002039900113
更新日期:2000-02-01 00:00:00
abstract::Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01805-x
更新日期:2020-07-01 00:00:00
abstract::Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402302
更新日期:1978-05-30 00:00:00
abstract::TonB-dependent receptors in concert with the TonB-ExbB-ExbD protein complex are responsible for the uptake of iron and substances such as vitamin B12 in several bacterial species. In this study, Tn5 mutagenesis of the sugarcane endophytic bacterium Gluconacetobacter diazotrophicus led to the isolation of a mutant with...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1045-4
更新日期:2015-03-01 00:00:00
abstract::The propensity of bacterium to sporulate or retain the vegetative form depends on the amount of phosphorylated Spo0A (Spo0A(-P)), regulated by Spo0E multigene family of phosphatases (Spo0E, YisI and YnzD). Phylogenetic analysis revealed that Spo0E multigene family of phosphatases (SMFP) descends in two distinct clades...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0446-7
更新日期:2009-03-01 00:00:00
abstract::An ammonia-oxidizing, autotroph growing, slender, curved rod was isolated from the soil of Hawaii. It is well distinguishable from any other nitrifying bacteria thus far described by their morphology. The cells are 1.1-3.0 mum long and 0.3-0.4 mum wide. They are motile by means of 1-4 subpolar to lateral flagella. In ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425099
更新日期:1976-05-03 00:00:00
abstract::Insect and mite pests are damaging stressors that are threatening the cultivation of tea plants, which result in enormous crop loss. Over the years, the effectiveness of synthetic pesticides has allowed for its prominent application as a control strategy. However, the adverse effects of synthetic pesticides in terms o...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-020-01862-7
更新日期:2020-08-01 00:00:00
abstract::The cold shock response in bacteria involves the expression of low-molecular weight cold shock proteins (CSPs) containing a nucleic acid-binding cold shock domain (CSD), which are known to destabilize secondary structures on mRNAs, facilitating translation at low temperatures. Caulobacter crescentus cspA and cspB are ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0602-8
更新日期:2010-09-01 00:00:00
abstract::Reciprocal qualitative and quantitative immunological experiments employing an anti-Pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the Streptococcus faecalis fructose diphosphate aldolase. The ext...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416976
更新日期:1976-10-11 00:00:00
abstract::A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0442-2
更新日期:2002-09-01 00:00:00
abstract::A gram-negative bacterium was isolated from activated sewage sludge with thiophene-2-carboxylate as the sole source of carbon and with nitrate as an electron acceptor. The isolate, strain NKK, was a motile, oxidase- and catalase-positive, rod-like bacterium with a G+C content of 61.7 mol%. Besides nitrate, oxygen coul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050575
更新日期:1998-04-01 00:00:00
abstract::Bacillus thuringiensis (Bt) is efficient, strongly specific, and avirulent to humans, making it one of the most popular biopesticides in the world. Bt LLP29 is a mosquitocidal strain that was first isolated from Magnolia denudata. To understand its molecular mechanism against mosquitoes, the genome of Bt LLP29 was seq...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01875-2
更新日期:2020-09-01 00:00:00
abstract::The alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sephadex G-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeric forms. Suc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425338
更新日期:1976-04-01 00:00:00
abstract::Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425354
更新日期:1987-07-01 00:00:00
abstract::The symbiotic bacteria Bradyrhizobium japonicum and Sinorhizobium meliloti, and the purple photosynthetic bacteria Rhodobacter capsulatus, Rhodovulum sulfidophilum, Roseobacter denitrificans and Rhodobacter sphaeroides possess homologous two-component regulatory systems, namely RegSR, ActSR, RegBA and PrrBA. The respe...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000207
更新日期:2000-11-01 00:00:00