Abstract：
:Expression of the gene encoding ribosome modulation factor (RMF), as measured using an rmf-lacZ gene fusion, increased with decreasing pH in exponential phase cultures of Escherichia coli. Expression was inversely proportional to the growth rate and independent of the acidifying agent used and it was concluded that expression of rmf was growth rate controlled in exponential phase under acid conditions. Increased rmf expression during exponential phase was not accompanied by the formation of ribosome dimers as occurs during stationary phase. Nor did it appear to have a significant effect on cell survival under acid stress since the vulnerability of an RMF-deficient mutant strain was similar to that of the parent strain. Ribosome degradation was increased in the mutant strain compared to the parent strain at pH 3.75. Also, the peptide elongation rate was reduced in the mutant strain but not the parent during growth under acid conditions. It is speculated that the function of RMF during stress-induced reduction in growth rate is two-fold: firstly to prevent reduced elongation efficiency by inactivating surplus ribosomes and thus limiting competition for available protein synthesis factors, and secondly to protect inactivated ribosomes from degradation.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
el-Sharoud WM,Niven GWdoi
10.1007/s00203-005-0025-0keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
18-24issue
1eissn
0302-8933issn
1432-072Xjournal_volume
184pub_type
杂志文章abstract：:Recently, a unique archaeal/bacterial community that grows in a macroscopically visible string-of-pearls-like structure in cold (~10 degrees C), sulfurous marsh water was discovered. Here, a new technique is described that allows the fast and reliable growth of these string-of-pearls-like microbial communities in larg...
journal_title：Archives of microbiology
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：2010-11-01 00:00:00
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doi：10.1007/BF00446663
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pub_type： 杂志文章
doi：10.1007/s00203-010-0562-z
更新日期：2010-05-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
doi：10.1007/s00203-002-0426-2
更新日期：2002-07-01 00:00:00
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doi：10.1007/s00203-016-1214-8
更新日期：2016-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-004-0747-4
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pub_type： 杂志文章,评审
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更新日期：2012-02-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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abstract：:The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2020-10-19 00:00:00
