Abstract：
:The pathway construction and allosteric regulation of phenylalanine and tyrosine biosynthesis was examined in Neisseria gonorrhoeae. A single 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase enzyme sensitive to feedback inhibition by L-phenylalanine was found. Chorismate mutase and prephenate dehydratase appear to co-exist as catalytic components of a bifunctional enzyme, known to be present in related genera. The latter enzyme activities were both feedback inhibited by L-phenylalanine. Prephenate dehydratase was strongly activated by L-tyrosine. NAD+-linked prephenate dehydrogenase and arogenate dehydrogenase activities coeluted following ion-exchange chromatography, suggesting their identity as catalytic properties of a single broad-specificity cyclohexadienyl dehydrogenase. Each dehydrogenase activity was inhibited by 4-hydroxyphenylpyruvate, but not by L-tyrosine. Two aromatic aminotransferases were resolved, one preferring the L-phenylalanine:2-ketoglutarate substrate combination and the other preferring the L-tyrosine: 2-ketoglutarate substrate combination. Each aminotransferase was also able to transaminate prephenate. The overall picture of regulation is one in which L-tyrosine modulates L-phenylalanine synthesis via activation of prephenate dehydratase. L-Phenylalanine in turn regulates early-pathway flow through inhibition of DAHP synthase. The recent phylogenetic positioning of N. gonorrhoeae makes it a key reference organism for emerging interpretations about aromatic-pathway evolution.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Berry A,Jensen RA,Hendry ATdoi
10.1007/BF00425071subject
Has Abstractpub_date
1987-01-01 00:00:00pages
87-94issue
2eissn
0302-8933issn
1432-072Xjournal_volume
149pub_type
杂志文章abstract：:Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402302
更新日期：1978-05-30 00:00:00
abstract：:1. An already published test method for detecting bactericidal substances in paper chromatograms was further improved. 2. In cultures of Cosmarium impressulum free from bacteria, two bactericidal substances were found in the ether extracts from the algae and two others in the extracts of the culture medium. The are ac...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428945
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abstract：:The ability of Methylosinus trichosporium OB3b, expressing soluble methane monooxygenase, to oxidize a range of ortho-substituted biphenyls was examined to better understand how substituents affect both the rate and products of oxidation in comparison to biphenyl. Inhibition of oxidation was observed over the tested s...
journal_title：Archives of microbiology
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doi：10.1007/s002030000170
更新日期：2000-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446529
更新日期：1975-12-31 00:00:00
abstract：:The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100286
更新日期：2001-07-01 00:00:00
abstract：:A new, phylogenetically distinct, dissimilatory, Fe(III)-reducing bacterium was isolated from surface sediment of a hydrocarbon-contaminated ditch. The isolate, designated strain PAL-1, was an obligately anaerobic, non-fermentative, motile, gram-negative vibrio. PAL-1 grew in a defined medium with acetate as electron ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050340
更新日期：1996-06-01 00:00:00
abstract：:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0878-6
更新日期：2013-05-01 00:00:00
abstract：:Short-chain aliphatic epoxides and ketones are two classes of toxic organic compounds formed biogenically and anthropogenically. In spite of their toxicity, these compounds are utilized as primary carbon and energy sources or are generated as intermediate metabolites in the metabolism of other compounds (e.g., alkenes...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050558
更新日期：1998-03-01 00:00:00
abstract：:Exoglucanase (exo-1,3-beta-D-glucan glycohydrolase, EC 3.2.1.56) activity secreted by Saccharomyces cerevisiae into the culture medium was separated by ion exchange chromatography into two glycoprotein isoenzymes which contributed 10% (exoglucanase I) and 90% (exoglucanase II) towards the total activity. Analysis of t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416596
更新日期：1989-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050688
更新日期：1999-01-01 00:00:00
abstract：:Chromate resistance in Ralstonia metallidurans CH34 is based on chromate efflux catalyzed by ChrA efflux pumps. The bacterium harbors two chromate resistance determinants, the previously known chr(1) on plasmid pMOL28 (genes chrI, chrB(1), chrA(1), chrC, chrE, chrF(1)) and chr(2) on the chromosome (genes chrB(2), chrA...
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pub_type： 杂志文章
doi：10.1007/s00203-002-0492-5
更新日期：2002-12-01 00:00:00
abstract：:During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea g...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01941-9
更新日期：2020-10-01 00:00:00
abstract：:Two gene clusters encoding similar formate dehydrogenases (FDH) were identified in Eubacterium acidaminophilum. Each cluster is composed of one gene coding for a catalytic subunit ( fdhA-I, fdhA-II) and one for an electron-transferring subunit ( fdhB-I, fdhB-II). Both fdhA genes contain a TGA codon for selenocysteine ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0508-1
更新日期：2003-01-01 00:00:00
abstract：:Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced beta-oxidation pathway showed the presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase. After isopicnic centrifugation in a ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050392
更新日期：1996-11-01 00:00:00
abstract：:We previously cloned the sigH gene encoding a stress-response sigma factor sigma(H) in Streptomyces coelicolor A3(2), located in an operon with the gene encoding proposed anti-sigma factor UshX. To clarify the in vivo function of sigma(H), a stable null mutant of sigH was prepared by homologous recombination. This mut...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0367-1
更新日期：2001-12-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000207
更新日期：2000-11-01 00:00:00
abstract：:The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0442-2
更新日期：2002-09-01 00:00:00
abstract：:In yeasts, several sensing systems localized to the plasma membrane which transduce information regarding the availability and quality of nitrogen and carbon sources and work in parallel with the intracellular nutrient-sensing systems, regulate the expression and activity of proteins involved in nutrient uptake and ut...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0022-3
更新日期：2005-11-01 00:00:00
abstract：:Inorganic polyphosphate (iPoP)-linear chains of up to hundreds of phosphate residues-is ubiquitous in nature and appears to be involved in many different cellular processes. In Saccharomyces cerevisiae, iPoP has been detected in high concentrations, especially after transfer of phosphate-deprived cells to a high-phosp...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0031-2
更新日期：2005-11-01 00:00:00
abstract：:Selenium nanoparticles (Se NPs) were synthesized using Saccharomyces cerevisiae yeast. Influences of different amounts of sodium selenite (5.0, 10.0, 15.0, 20.0, and 25 µg) were evaluated on growth of yeast during incubation at 32 °C, during 4 days. UV-Vis spectroscopy results have shown that synthesized Se NPs had br...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01831-0
更新日期：2020-07-01 00:00:00
abstract：:The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01833-y
更新日期：2020-07-01 00:00:00
abstract：:The production of secondary metabolites with antibiotic properties is a common characteristic to Bacillus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities with medicinal and agricultural interests such as antibiotic. Bacillus sp. fmbJ has been found to produc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0939-x
更新日期：2014-02-01 00:00:00
abstract：:An unidentified filamentous purple bacterium, probably belonging to a new genus or even a new family, is found in close association with the filamentous, mat-forming cyanobacterium Microcoleus chthonoplastes in a hypersaline pond at Guerrero Negro, Baja California Sur, Mexico, and in Solar Lake, Sinai, Egypt. This or...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00463477
更新日期：1987-01-01 00:00:00
abstract：:Mycothiol is the predominant thiol in most actinomycetes, including Mycobacterium tuberculosis, and appears to play a role analogous to glutathione, which is not found in these bacteria. The enzymes involved in mycothiol biosynthesis are of interest as potential targets for new drugs directed against tuberculosis. In ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0462-y
更新日期：2002-11-01 00:00:00
abstract：:The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050505
更新日期：1997-10-01 00:00:00
abstract：:Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100350
更新日期：2001-12-01 00:00:00
abstract：:Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the max...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050731
更新日期：1999-05-01 00:00:00
abstract：:In addition to rhizobia, other non-symbiotic endophytic bacteria also have been simultaneously isolated from the same root nodules. The existence of non-symbiotic endophytic bacteria in leguminous root nodules is a universal phenomenon. The vast majority of studies have detected endophytic bacteria in other plant tiss...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01752-7
更新日期：2020-03-01 00:00:00
abstract：:Among proteins specifically found in most gram-positive bacteria of the phylum Firmicutes, conserved proteins of the family pfam06177-DUF988-COG4708 are of unknown function. The citrulline cluster-linked (ccl) gene of Lactobacillus plantarum codes one such protein and is adjacent to the citrulline biosynthesis operon ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0774-9
更新日期：2005-08-01 00:00:00
