Presence of nucleoside triphosphates and calcium associated with mycobacteriophage 13.

Abstract:

:The association of nucleoside triphosphate molecules and calcium ions with purified particles of mycobacteriophage 13 has been documented. The content of nucleoside triphosphate has been determined to be 118 molecules per phage particle by equilibrium dialysis against labelled ATP or 148 molecules per phage particle by the direct determination of labelled nucleoside triphosphate. The concentration of bound Ca2+ exhibited a high degree of variation between different batches, which may be due to the nonspecific binding of Ca2+ by the virus particles. However, the tightly bound Ca2+ not removable by dialysis against calcium-specific chelating agent, showed a constant value of 2985 atoms/phage particle.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Karnik SS,Gopinathan KP

doi

10.1007/BF00527071

subject

Has Abstract

pub_date

1981-09-01 00:00:00

pages

50-3

issue

1

eissn

0302-8933

issn

1432-072X

journal_volume

130

pub_type

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