Purification and properties of a fructose-1,6-diphosphate activated L-lactate dehydrogenase from Staphylococcus epidermidis.

abstract：:We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...

journal_title：Archives of microbiology

authors： Yu Z,Zeng Z,Huang Z,Lian J,Yang J,Deng Q,Zeng W

更新日期：2010-08-01 00:00:00

abstract：:Light-limited batch cultures of Synechococcus spp. strains PCC 6301 and WH 8103 exhibited a positive correlation between the specific growth rate and the cellular content of both RNA and DNA. The ratio of RNA to DNA increased with the growth rate in Synechococcus sp. strain WH 8103, as it does in heterotrophic bacteri...

journal_title：Archives of microbiology

authors： Lepp PW,Schmidt TM

更新日期：1998-09-01 00:00:00

abstract：:The halophilic archaeal strain GX71(T) was isolated from the Gangxi marine solar saltern near the Weihai city of Shandong Province, China. Cells of the strain were pleomorphic and lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. Strain GX71(T) was able to grow at 25-45 °C (optimum 30 ...

journal_title：Archives of microbiology

authors： Zhang WJ,Cui HL

更新日期：2014-06-01 00:00:00

abstract：:Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...

journal_title：Archives of microbiology

authors： Schleicher L,Fritz G,Seifert J,Steuber J

更新日期：2020-07-01 00:00:00

abstract：:An organism from a goldfish aquarium, isolated on barbital medium, was found to be a Gram-positive coccus which divided in alternating planes, often appearing as a doublet or as a tetrad with adjacent sides flattened. It grew well, although slowly, on rich solid medium (LB agar) and in liquid brain-heart infusion at r...

journal_title：Archives of microbiology

authors： Poston JM

更新日期：1993-01-01 00:00:00

abstract：:A gram-negative bacterium was isolated from activated sewage sludge with thiophene-2-carboxylate as the sole source of carbon and with nitrate as an electron acceptor. The isolate, strain NKK, was a motile, oxidase- and catalase-positive, rod-like bacterium with a G+C content of 61.7 mol%. Besides nitrate, oxygen coul...

journal_title：Archives of microbiology

authors： Bambauer A,Rainey FA,Stackebrandt E,Winter J

更新日期：1998-04-01 00:00:00

abstract：:Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...

journal_title：Archives of microbiology

authors： Gustafsson MC,Palmer CN,Wolf CR,von Wachenfeldt C

更新日期：2001-12-01 00:00:00

abstract：:The anaerobic fungus Piromyces sp. strain E2 produces extracellular cellulolytic enzymes present both in a high molecular mass (HMM) complex or as individual proteins. Although the HMM complex was present in the culture fluid during all growth stages, the highest amounts of complex were obtained when cultures were har...

journal_title：Archives of microbiology

authors： Dijkerman R,Op den Camp HJ,Van der Drift C,Vogels GD

更新日期：1997-02-01 00:00:00

abstract：:A LD-carboxypeptidase from Escherichia coli K 12 was isolated by Tris-EDTA treatment and purified to electrophoretic homogeneity by DEAE-cellulose chromatography. The enzyme has a molecular weight of approximately 12,000 as determined by sodium dodecyl sulfate-polyacrylamide electrophoresis and by Sephadex G-100 gel f...

journal_title：Archives of microbiology

authors： Metz R,Henning S,Hammes WP

更新日期：1986-03-01 00:00:00

abstract：:Glyoxalase I is a ubiquitous enzyme that detoxifies methylglyoxal, which is derived from glycolysis but inhibits the growth of cells from microorganisms to mammals. Here, the structural gene for glyoxalase I ( glo1(+)) from the fission yeast Schizosaccharomyces pombe was identified. Disruption of glo1(+) enhanced susc...

journal_title：Archives of microbiology

authors： Takatsume Y,Izawa S,Inoue Y

更新日期：2004-05-01 00:00:00

abstract：:The prpC and prkC genes encode two proteins with opposing activities, eukaryotic-like protein phosphatase and protein kinase involved in the sporulation and biofilm formation processes. The prpC gene overlaps 3 bp of the prkC gene; prkC is followed by the essential gene, yloQ. The organisation of the prpC, prkC and yl...

journal_title：Archives of microbiology

authors： Iwanicki A,Hinc K,Seror S,Wegrzyn G,Obuchowski M

更新日期：2005-09-01 00:00:00

abstract：:Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...

journal_title：Archives of microbiology

authors： O'Donnell AG,Minnikin DE,Goodfellow M,Piot P

更新日期：1984-05-01 00:00:00

abstract：:To analyze if chemical cell wall alterations contribute to penicillin-induced bacteriolysis, changes in the amount, stability, and chemical composition of staphylococcal cell walls were investigated. All analyses were performed before onset of bacteriolysis i.e. during the first 60 min following addition of different ...

journal_title：Archives of microbiology

authors： Sidow T,Johannsen L,Labischinski H

更新日期：1990-01-01 00:00:00

abstract：:A neutral protease (npr) (designated Bae16) toxic to nematodes was purified to homogeneity from the strain Bacillus nematocida. The purified protease showed a molecular mass of approximately 40 kDa and displayed optimal activity at 55 degrees C, pH 6.5. Bioassay experiments demonstrated that this purified protease cou...

journal_title：Archives of microbiology

authors： Niu Q,Huang X,Zhang L,Li Y,Li J,Yang J,Zhang K

更新日期：2006-06-01 00:00:00

abstract：:The gliding bacterium Herpetosiphon giganteus Hp a2 was shown to contain gamma-carotene, one monoglucosyloxy and two new diglucosyloxy carotenoids with a x-O-acyldiglucosyloxy carotenoid as main component. Flexirubin-like pigments could not be detected in this organism. As in the Myxobacterales and Cytophagales (Klein...

journal_title：Archives of microbiology

authors： Kleinig H,Reichenbach H

更新日期：1977-04-01 00:00:00

abstract：:The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. T...

journal_title：Archives of microbiology

authors： Nieto-Peñalver CG,Bertini EV,de Figueroa LI

更新日期：2012-07-01 00:00:00

abstract：:A novel pathway for 2-aminophenol metabolism by Pseudomonas sp. AP-3 is proposed. The proposed pathway is similar to that known for meta-cleavage of catechol except that one of the hydroxyl groups on the metabolites is replaced by an amino group. During the degradation of 2-aminophenol, 2-amino-2,4-pentadienoic acid i...

journal_title：Archives of microbiology

authors： Takenaka S,Murakami S,Shinke R,Aoki K

更新日期：1998-08-01 00:00:00

abstract：:The radiation resistant bacteria Micrococcus radiophilus and M. radioproteolyticus were studied by thin sectioning and freez-etching techniques and the two species were found to be similar in the fine structure. The only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched pr...

journal_title：Archives of microbiology

authors： Sleytr UB,Silva MT,Kocur M,Lewis NF

更新日期：1976-04-01 00:00:00

abstract：:Cryptococcosis is a common opportunistic infection in patients with advanced HIV infection and may also affect immunocompetent patients. The available antifungal agents are few and other options are needed for the cryptococcosis treatment. In this work, we first analyzed the virulence of twelve C. neoformans and C. ga...

journal_title：Archives of microbiology

authors： de Castro Spadari C,da Silva de Bastiani FWM,Pisani PBB,de Azevedo Melo AS,Ishida K

更新日期：2020-05-01 00:00:00

abstract：:CO2 is required continuously during germination of Streptomyces viridochromogenes spores. Spores incubated in a defined germination medium in the absence of CO2 remain phase bright and do not release spore carbon. In the presence of CO2, the spores initiate germination accompanied by loss of refractility and spore car...

journal_title：Archives of microbiology

authors： Grund AD,Ensign JC

更新日期：1978-09-01 00:00:00

abstract：:Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced beta-oxidation pathway showed the presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase. After isopicnic centrifugation in a ...

journal_title：Archives of microbiology

authors： Valenciano S,Lucas JR,Pedregosa A,Monistrol IF,Laborda F

更新日期：1996-11-01 00:00:00

abstract：:After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...

journal_title：Archives of microbiology

authors： Habte M,Alexander M

更新日期：1977-06-20 00:00:00

abstract：:Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2'R,6'R)-decaprenoxanthin di-(beta-D)-glucoside, but in three-fold higher amounts. In addition, dec...

journal_title：Archives of microbiology

authors： Krubasik P,Takaichi S,Maoka T,Kobayashi M,Masamoto K,Sandmann G

更新日期：2001-09-01 00:00:00

abstract：:Over 2,800 Tn 5 insertion mutants of Salmonella enterica sv. Typhimurium were screened for the loss of ability to suppress the multiplication of a spectinomycin-resistant (Spc(r)) but otherwise isogenic S. enterica sv. Typhimurium strain, when the Spc(r) mutant was added to 24-h LB broth cultures of the mutants. Selec...

journal_title：Archives of microbiology

authors： Rychlik I,Martin G,Methner U,Lovell M,Cardova L,Sebkova A,Sevcik M,Damborsky J,Barrow PA

更新日期：2002-12-01 00:00:00

abstract：:The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...

journal_title：Archives of microbiology

authors： Arafat HH,Abu-Tahon MA,Isaac GS

更新日期：2020-10-19 00:00:00

abstract：:The pathway construction and allosteric regulation of phenylalanine and tyrosine biosynthesis was examined in Neisseria gonorrhoeae. A single 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase enzyme sensitive to feedback inhibition by L-phenylalanine was found. Chorismate mutase and prephenate dehydratase ap...

journal_title：Archives of microbiology

authors： Berry A,Jensen RA,Hendry AT

更新日期：1987-01-01 00:00:00

abstract：:Short-chain aliphatic epoxides and ketones are two classes of toxic organic compounds formed biogenically and anthropogenically. In spite of their toxicity, these compounds are utilized as primary carbon and energy sources or are generated as intermediate metabolites in the metabolism of other compounds (e.g., alkenes...

journal_title：Archives of microbiology

authors： Ensign SA,Small FJ,Allen JR,Sluis MK

更新日期：1998-03-01 00:00:00

abstract：:DNA acquisition promotes the spread of resistance to antibiotics and virulence among bacteria. It is also linked to several natural phenomena including recombination, genome dynamics, adaptation and speciation. Horizontal DNA transfer between bacteria occurs via conjugation, transduction or competence for natural tran...

journal_title：Archives of microbiology

authors： Bakkali M

更新日期：2013-04-01 00:00:00

abstract：:Ever since the development of the first antibiotic compound with anticancer potential, researchers focused on isolation and characterization of prospective microbial natural products with potential anti-infective and anticancer activities. The present work describes the production of bioactive metabolites by heterotro...

journal_title：Archives of microbiology

authors： Kizhakkekalam VK,Chakraborty K

更新日期：2020-11-02 00:00:00

abstract：:Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...

journal_title：Archives of microbiology

authors： Hasona A,Self WT,Shanmugam KT

更新日期：2001-03-01 00:00:00