Abstract:
:Short-chain aliphatic epoxides and ketones are two classes of toxic organic compounds formed biogenically and anthropogenically. In spite of their toxicity, these compounds are utilized as primary carbon and energy sources or are generated as intermediate metabolites in the metabolism of other compounds (e.g., alkenes, alkanes, and secondary alcohols) by a number of diverse bacteria. One bacterium capable of using both classes of compounds is the gram-negative aerobe Xanthobacter strain Py2. Studies of epoxide and ketone (acetone) metabolism by Xanthobacter strain Py2 have revealed a central role for CO2 in these processes. Both classes of compounds are metabolized by carboxylation reactions that produce beta-keto acids as products. The epoxide- and ketone-converting enzymes are distinct carboxylases with molecular properties and cofactor requirements unprecedented for other carboxylases. Epoxide carboxylase is a four-component multienzyme complex that requires NADPH and NAD+ as cofactors. In the course of epoxide carboxylation, a transhydrogenation reaction occurs wherein NADPH undergoes oxidation and NAD+ undergoes reduction. Acetone carboxylase is a multimeric (three-subunit) ATP-dependent enzyme that forms AMP and inorganic phosphate as ATP hydrolysis products in the course of acetone carboxylation. Recent studies have demonstrated that acetone metabolism in diverse anaerobic bacteria (sulfate reducers, denitrifiers, phototrophs, and fermenters) also proceeds by carboxylation reactions. ATP-dependent acetone carboxylase activity has been demonstrated in cell-free extracts of the anaerobic acetone-utilizers Rhodobacter capsulatus, Rhodomicrobium vannielii, and Thiosphaera pantotropha. These studies have identified new roles for CO2 as a cosubstrate in the metabolism of two classes of important xenobiotic compounds. In addition, two new classes of carboxylases have been identified, the investigation of which promises to reveal new insights into biological strategies for the fixation of CO2 to organic substrates.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Ensign SA,Small FJ,Allen JR,Sluis MKdoi
10.1007/s002030050558subject
Has Abstractpub_date
1998-03-01 00:00:00pages
179-87issue
3eissn
0302-8933issn
1432-072Xjournal_volume
169pub_type
杂志文章,评审abstract::The free tryptophan pool and the levels of two enzymes of tryptophan biosynthesis (anthranilate synthase and indoleglycerolphosphate synthase)have been determined in a wild type strain of Saccharomyces cerevisiae and in mutants with altered regulatory properties. ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446842
更新日期:1976-03-19 00:00:00
abstract::In an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16S rDNA sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. All except one st...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050350
更新日期:1996-07-01 00:00:00
abstract::TonB-dependent receptors in concert with the TonB-ExbB-ExbD protein complex are responsible for the uptake of iron and substances such as vitamin B12 in several bacterial species. In this study, Tn5 mutagenesis of the sugarcane endophytic bacterium Gluconacetobacter diazotrophicus led to the isolation of a mutant with...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1045-4
更新日期:2015-03-01 00:00:00
abstract::Pyrophosphate (PPi) content of Escherichia coli is increased manyfold when the growth of the cells is limited by inhibition of the synthesis of nucleotides. The accumulated PPi is immediately degraded when inhibition is released and growth allowed to resume. Other tested nutritional deficiencies (starvation of carbon ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409846
更新日期:1983-11-01 00:00:00
abstract::Cellulomonas uda (DSM 20108/ATCC 21399) is one of the few described cellulolytic facultative anaerobes. Based on these characteristics, we initiated a physiological study of C. uda with the aim to exploit it for cellulase production in simple bioreactors with no or sporadic aeration. Growth, cellulase activity and fer...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1230-8
更新日期:2016-10-01 00:00:00
abstract::Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1247-z
更新日期:2016-11-01 00:00:00
abstract::Virulence factors of H. pylori, such as outer inflammatory protein A (oipA), are closely involved in the development of gastric diseases such as chronic gastritis and gastric cancer. The functional status of oipA is regulated by a repair mechanism based on CT dinucleotide repeats that influence the reading frame, thus...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-018-1580-5
更新日期:2019-01-01 00:00:00
abstract::Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0238-5
更新日期:2007-08-01 00:00:00
abstract::Acinetobacter species remain alive in hospitals on various surfaces, both dry and moist, forming an important source of hospital infections. These bacteria are naturally resistant to many antibiotic classes. Although the role of the quorum sensing system in regulating the virulence factors of Acinetobacter species has...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1408-8
更新日期:2017-12-01 00:00:00
abstract::The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429336
更新日期:1977-03-01 00:00:00
abstract::The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00421893
更新日期:1980-05-01 00:00:00
abstract::The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0276-z
更新日期:2007-12-01 00:00:00
abstract::Plasmid pJP4 encoding the ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (Tfd+) was transferred by conjugation from Escherichia coli JMP397 to various lithoautotrophic strains of Alcaligenes eutrophus and to the autotrophic bacterium Pseudomonas oxalaticus. The herbicide-degrading function of the plas...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00407938
更新日期:1983-02-01 00:00:00
abstract::Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0414-2
更新日期:2008-12-01 00:00:00
abstract::The tricarboxylic acid (TCA) cycle is one of the major routes of carbon catabolism in Bacillus subtilis. The syntheses of the enzymes performing the initial reactions of the cycle, citrate synthase, and aconitase, are synergistically repressed by rapidly metabolizable carbon sources and glutamine. This regulation invo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0078-0
更新日期:2006-03-01 00:00:00
abstract::Rather recently it has become clear that prokaryotes (Archaea and Bacteria) are able to glycosylate proteins. A literature survey revealed the different types of glycoproteins. They include mainly surface layer (S-layer) proteins, flagellins, and polysaccharide-degrading enzymes. Only in a few cases is structural info...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030050484
更新日期:1997-09-01 00:00:00
abstract::Video-enhanced light microscopy of the apical and subapical regions of growing hyphae of several fungal species revealed the existence of momentary synchronized motions of subcellular organelles. First discovered in a temperature-sensitive morphological mutant (ramosa-1) of Aspergillus niger, these seemingly spontaneo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0771-z
更新日期:2005-05-01 00:00:00
abstract::Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00444661
更新日期:1989-01-01 00:00:00
abstract::Sulfolane (tetrahydrothiophene-1,1-dioxide) is used in the Sulfinol process for natural gas sweetening. At many sour-gas processing plants spills, landfills and leakage from unlined surface storage ponds have contaminated groundwaters with sulfolane. Due to its high water solubility and mobility in aquifers, sulfolane...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000184
更新日期:2000-07-01 00:00:00
abstract::Gene probe hybridization was used to determine distribution and expression of co-metabolic genes at a contaminated site as it underwent in situ methanotrophic bioremediation of trichloroethylene (TCE). The bioremediation strategies tested included a series of air, air:methane, and air:methane:nutrient pulses of the te...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0445-8
更新日期:2009-03-01 00:00:00
abstract::A monoclonal antibody obtained by immunization of mice with heat-killed cells of Listeria monocytogenes serotype 4d showed reactivity towards a protein (P45) from L. monocytogenes with an apparent molecular mass of 45 kDa. This protein was detected in the culture supernatant and at the cell surface of L. monocytogenes...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050003
更新日期:2000-01-01 00:00:00
abstract::The biosynthesis of two flavoproteins, 6-hydroxy-D-nicotine oxidase with covalently bound FAD and 6-hydroxy-L-nicotine oxidase containing non-covalently bound FAD, was studied in wild-type cells and in a riboflavin-requiring mutant of Arthrobacter oxidans. In the mutant cells, the rate of synthesis and the maximal act...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00407929
更新日期:1978-10-04 00:00:00
abstract::Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00414437
更新日期:1989-01-01 00:00:00
abstract::The bacteriocinogenic strain of Enterococcus faecalis EJ97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050678
更新日期:1998-12-01 00:00:00
abstract::The aim of this study was to investigate the viable but nonculturable (VBNC) state of the bacterium. Vibrio alginolyticus VIB283 was cultured in sterilized seawater microcosm at 4 degrees C. Culturability of the cells in the microcosm was monitored by spread plate count (PC) on 2216E agar, PCs declined to undetectable...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0246-5
更新日期:2007-09-01 00:00:00
abstract::The family Chlamydiaceae contains nine species pathogenic to humans and animals, but their routine identification is hampered by inadequate detection methods. In an attempt to find a new region for PCR detection and discrimination of the Chlamydiaceae species, the 3' end of the omp2 gene of Chlamydiaceae has been exam...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0757-2
更新日期:2005-03-01 00:00:00
abstract::The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0971-5
更新日期:2014-05-01 00:00:00
abstract::Some unicellular N(2)-fixing cyanobacteria have recently been found to lack a functional photosystem II of photosynthesis. Such organisms, provisionally termed UCYN-A, of the oceanic picoplanktion are major contributors to the global marine N-input by N(2)-fixation. Since their photosystem II is inactive, they can per...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-010-0621-5
更新日期:2010-10-01 00:00:00
abstract::Rhodopseudomonas capsulata produces both an intermediate (I) and a large (L) form of ribulose-1,5-bisphosphate carboxylase/oxygenase. Both forms are derepressed under CO2-limiting conditions. The L-form of the enzyme is completely repressed when the culture is grown either photoautotrophically or photoheterotrophicall...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402127
更新日期:1984-07-01 00:00:00
abstract::Ostracods collected from shallow coral reefs in the Bahamas were found to exhibit blue light-stimulated orange fluorescence at night. Fluorescent spectra revealed the presence of orange fluorescence with a maximum emission at ~595 nm on the carapace of these ostracods, while scanning electron microscopy revealed a mor...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-013-0911-9
更新日期:2013-09-01 00:00:00