Abstract：
:The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the clones used to determine the genomic sequence of the cyanobacterium, Anabaena sp. strain PCC 7120. One integrating vector is a mobilizable BAC vector that was used both to generate bridging clones and to complement transposon mutations. Upon addition of a cassette that permits mobilization and selection, pUC-based sequencing clones can also integrate into the genome and thereupon complement transposon mutations. The replicating vectors are based on cyanobacterial plasmid pDU1, whose sequence we report, and on broad-host-range plasmid RSF1010. The RSF1010- and pDU1-based vectors provide the opportunity to express different genes from either cell-type-specific or -generalist promoters, simultaneously from different plasmids in the same cyanobacterial cells. We show that pDU1 ORF4 and its upstream region play an essential role in the replication and copy number of pDU1, and that ORFs alr2887 and alr3546 (hetF A) of Anabaena sp. are required specifically for fixation of dinitrogen under oxic conditions.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Wolk CP,Fan Q,Zhou R,Huang G,Lechno-Yossef S,Kuritz T,Wojciuch Edoi
10.1007/s00203-007-0276-zsubject
Has Abstractpub_date
2007-12-01 00:00:00pages
551-63issue
6eissn
0302-8933issn
1432-072Xjournal_volume
188pub_type
杂志文章abstract：:Many pathogenic bacteria express filamentous appendages, termed pili, on their surface. These organelles function in several important bacterial processes, including mediating bacterial interaction with, and colonization of the host, signalling events, locomotion, DNA uptake, electric conductance, and biofilm formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-015-1117-0
更新日期：2015-08-01 00:00:00
abstract：:Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1166-4
更新日期：2016-03-01 00:00:00
abstract：:Expression of unnecessary proteins is known to reduce the growth rate of Escherichia coli. This reduction in growth rate, due to the diversion of cellular resources from making essential proteins, is called cost. Cellular resources depend upon the macromolecular content of the cell. Cost phenomenon lies in the process...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0967-1
更新日期：2014-04-01 00:00:00
abstract：:The association of nucleoside triphosphate molecules and calcium ions with purified particles of mycobacteriophage 13 has been documented. The content of nucleoside triphosphate has been determined to be 118 molecules per phage particle by equilibrium dialysis against labelled ATP or 148 molecules per phage particle b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00527071
更新日期：1981-09-01 00:00:00
abstract：:Stress resistance and fermentative capability are important quality characteristics of baker's yeast. In the present study, we examined protective effects of exogenous alpha-ketoglutarate (AKG), an intermediate of the tricarboxylic acid cycle and amino acid metabolism, against freeze-thaw and carbohydrate-induced stre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1423-9
更新日期：2018-01-01 00:00:00
abstract：:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0878-6
更新日期：2013-05-01 00:00:00
abstract：:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428588
更新日期：1977-05-13 00:00:00
abstract：:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombinati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409661
更新日期：1989-01-01 00:00:00
abstract：:Cellulomonas uda (DSM 20108/ATCC 21399) is one of the few described cellulolytic facultative anaerobes. Based on these characteristics, we initiated a physiological study of C. uda with the aim to exploit it for cellulase production in simple bioreactors with no or sporadic aeration. Growth, cellulase activity and fer...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1230-8
更新日期：2016-10-01 00:00:00
abstract：:Mycoplasma pneumoniae and Mycoplasma genitalium are important causative agents of infections in humans. Like all other mycoplasmas, these species possess genomes that are significantly smaller than that of other prokaryotes. Moreover, both organisms possess an exceptionally compact set of DNA recombination and repair-...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-020-02041-4
更新日期：2020-09-24 00:00:00
abstract：:Periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. Sequential colonization of a broad range of bacteria, including Fusobacterium nucleatum and Porphyromonas gingivalis, is an important phenomenon in this disease mode...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1223-7
更新日期：2016-08-01 00:00:00
abstract：:The entomopathogenic mushroom Cordyceps militaris is a storehouse of various medicinal compounds and pharmacological effects. However, the high frequency of strain degeneration during subculture and preservation severely limits the large-scale production of C. militaris. DNA methylation is an important epigenomic modi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01621-3
更新日期：2019-04-01 00:00:00
abstract：:For the low molecular weight laccases II and III of Podospora anserina the kinetic parameters Michaelis constant (KM) and maximum reaction velocity (V) were determined polarographically under pH optimum conditions for representative substrates of different substitution patterns. Laccase II showed two peaks in its pH o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446663
更新日期：1977-02-04 00:00:00
abstract：:It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has open...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-011-0733-6
更新日期：2012-01-01 00:00:00
abstract：:Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050589
更新日期：1998-05-01 00:00:00
abstract：:Grasses of the Urochloa genus have been widely used in crop-livestock integration systems or as cover crops in no-till systems such as in rotation with maize. Some species of Urochloa have mechanisms to reduce nitrification. However, the responses of microbial functions in crop-rotation systems with grasses and its co...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01910-2
更新日期：2020-09-01 00:00:00
abstract：:To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000236
更新日期：2001-01-01 00:00:00
abstract：:Chemoautotrophic endosymbionts residing in Solemya velum gills provide this shallow water clam with most of its nutritional requirements. The cbb gene cluster of the S. velum symbiont, including cbbL and cbbS, which encode the large and small subunits of the carbon-fixing enzyme ribulose 1,5-bisphosphate carboxylase/o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0689-x
更新日期：2004-09-01 00:00:00
abstract：:Temperature dependency of sexual agglutination in Saccharomyces cerevisiae was found. Of 31 strains tested, which showed normal agglutination when cultured at 25 degrees C, 29 strains lost their sexual agglutinability when they were grown at 37 degrees C. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446875
更新日期：1977-09-28 00:00:00
abstract：:Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0457-z
更新日期：2009-04-01 00:00:00
abstract：:A novel Gram-stain positive, aerobic, non-motile Actinobacterium, designated strain SB3404T, was isolated from saltern soil collected from Boncuk Saltern, Sungurlu-Çorum, Turkey, and subjected to a polyphasic taxonomic approach. The organism has shown to have phylogenetic, chemotaxonomic, cultural and morphological pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02036-1
更新日期：2021-01-01 00:00:00
abstract：:The gut microbiota inhabits the animal intestinal tract, and dysbiosis of the gut microbiota may result in disease. Senecio scandens has pharmaceutical antibacterial activities and is regarded as a broad-spectrum antibiotic in traditional Chinese medicine. Extracts of S. scandens are reported to show strong antimicrob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02144-y
更新日期：2021-01-04 00:00:00
abstract：:The anaerobic fungus Piromyces sp. strain E2 produces extracellular cellulolytic enzymes present both in a high molecular mass (HMM) complex or as individual proteins. Although the HMM complex was present in the culture fluid during all growth stages, the highest amounts of complex were obtained when cultures were har...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:The biological effects of UV radiation of different wavelengths (UVA, UVB and UVC) were assessed in nine bacterial isolates displaying different UV sensitivities. Biological effects (survival and activity) and molecular markers of oxidative stress [DNA strand breakage (DSB), generation of reactive oxygen species (ROS)...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0847-5
更新日期：2013-01-01 00:00:00
abstract：:Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00444661
更新日期：1989-01-01 00:00:00
abstract：:Milk containing antibiotics is used as cost-effective feed for calves, which may lead to antibiotic residues-associated food safety problems. This study aims to investigate the influence of antibiotics on rumen microbes. Through metagenomic sequencing, the rumen microbial communities of calves fed with pasteurized mil...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1311-8
更新日期：2017-04-01 00:00:00
abstract：:We report on the genome-wide response, based on DNA microarrays, of the cyanobacterium Synechocystis sp. PCC 6803 wild type and DeltasigB to a 15 min heat shock. Approximately 9% of the genes in wild type and DeltasigB were significantly regulated (P < 0.001) following this treatment, with chaperones induced the most....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0138-0
更新日期：2006-10-01 00:00:00
abstract：:An Arthrobacter sp. growing on 4-Chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. Protocatechuic acid is further attacked by "meta"-cleavage. During growth of the Arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690235
更新日期：1976-11-02 00:00:00
abstract：:Outer membrane vesicles (OMVs) are released from the outer membrane of Gram-negative bacteria. Moreover, Gram-positive bacteria also produce membrane-derived vesicles. As OMVs transport several bacterial components, especially from the cell envelope, their interaction with the host cell, with other bacteria or as immu...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-014-1042-7
更新日期：2015-01-01 00:00:00
abstract：:Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402302
更新日期：1978-05-30 00:00:00
