Abstract:
:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v] NaCl), a proteomic approach was used. The ATR and OTR of two L. monocytogenes cheese dairy isolates (strain T8, serotype 4b and A9, serotype 1/2b or 3b) were determined. The proteomes of the adapted and non-adapted cultures were evaluated by 2-DE. One strain displayed an ATR, but not an OTR and the other displayed an OTR, but not an ATR. The ATR positive strain showed the over-production of proteins related with protein synthesis, protein folding, attainment of reduction power, ribose production and cell wall. In contrast, in the OTR-positive-strain proteins related with glycolysis, general stress and detoxification were identified.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Melo J,Andrew PW,Faleiro MLdoi
10.1007/s00203-013-0878-6subject
Has Abstractpub_date
2013-05-01 00:00:00pages
339-48issue
5eissn
0302-8933issn
1432-072Xjournal_volume
195pub_type
杂志文章abstract::Polysomes were isolated both from growing gametophytes of Allomyces arbuscula and from gametangia prepared from mycelia at different periods during gametogenesis. Analysis of polysomes by sucrose gradients showed that ribosomes present in the gametangia monosome pool were shifted into polysomes. This shift was found t...
journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
pub_type: 杂志文章
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journal_title:Archives of microbiology
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