Abstract:
:For the low molecular weight laccases II and III of Podospora anserina the kinetic parameters Michaelis constant (KM) and maximum reaction velocity (V) were determined polarographically under pH optimum conditions for representative substrates of different substitution patterns. Laccase II showed two peaks in its pH optimum curve, each with a different substrate specificity, indicating structural differences to laccase III which exhibits only one broad peak. Under optimum conditions the affinities of various substrates are determined by their substitution patterns: high affinity for simple o- and p-diphenols, low affinity for m-henols. The maximal velocity remains largely uninfluenced. This study of the effect of substitution on substrate utilization leads to the assumption that there is no specific reactive site for m-phenols in either laccase. Oxidation of m-phenols, however, takes only place at high pH values.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Hoffmann P,Esser Kdoi
10.1007/BF00446663keywords:
subject
Has Abstractpub_date
1977-02-04 00:00:00pages
111-4issue
1eissn
0302-8933issn
1432-072Xjournal_volume
112pub_type
杂志文章abstract::The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00428588
更新日期:1977-05-13 00:00:00
abstract::A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0442-2
更新日期:2002-09-01 00:00:00
abstract::Hopane glycolipid, isolated from Bacillus acidocaldarius, was studied in its ability to support growth from Mycoplasma mycoides var. capri as substitute for sterols. All attempts to culture Mycoplasma on hopane glycolipid failed. When added together with cholesterol, the effect of HGL was a reduction in growth rate. T...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413519
更新日期:1982-12-01 00:00:00
abstract::After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492022
更新日期:1977-06-20 00:00:00
abstract::Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00444661
更新日期:1989-01-01 00:00:00
abstract::Chemical mutagenesis of the nucleotide-producing strain Corynebacterium ammoniagenes ATCC 6872 with N-methyl-N-nitro-N-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. A rapid assay for the allosterically regulated Mn-ribonucleotide reductase (RRase) was developed with ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050450
更新日期:1997-05-01 00:00:00
abstract::The utilization of carnitine and carnitine derivatives (O-acylcarnitines, carnitine carboxylderivatives) and structure-related trimethylammonium-compounds (betaines and nitrogen-bases) by Acinetobacter calcoaceticus was studied by means of the control of growth and the quantitative detection of metabolites. The strain...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429336
更新日期:1977-03-01 00:00:00
abstract::All Serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. DNA fragments of the two hemolysin genes hybridized with the chromosomal DNA of S. marcescens, S. liquefaciens, S. kiliensis, S. grimesii, S. proteamaculans, S. plym...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248958
更新日期:1990-01-01 00:00:00
abstract::The ADPglucose pyrophosphorylases from Rhodopseudomonas sphaeroides and Rhodopseudomonas gelatinosa are activated by fructose-6-phosphate, pyruvate and fructose-1,6 biophosphate-P2. The effects of the activators are to increase significantly the Vmax of ADPglucose synthesis and to lower the S0.5 values (concentration ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00421887
更新日期:1980-05-01 00:00:00
abstract::The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050777
更新日期:1999-12-01 00:00:00
abstract::Among proteins specifically found in most gram-positive bacteria of the phylum Firmicutes, conserved proteins of the family pfam06177-DUF988-COG4708 are of unknown function. The citrulline cluster-linked (ccl) gene of Lactobacillus plantarum codes one such protein and is adjacent to the citrulline biosynthesis operon ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0774-9
更新日期:2005-08-01 00:00:00
abstract::A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the p...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447134
更新日期:1975-10-27 00:00:00
abstract::An organism from a goldfish aquarium, isolated on barbital medium, was found to be a Gram-positive coccus which divided in alternating planes, often appearing as a doublet or as a tetrad with adjacent sides flattened. It grew well, although slowly, on rich solid medium (LB agar) and in liquid brain-heart infusion at r...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00288712
更新日期:1993-01-01 00:00:00
abstract::We cloned the Kluyveromyces lactis KlNTH1 gene, which encodes neutral trehalase. It showed 65.2% and 68.5% identity at nucleotide and amino acid sequence level, respectively, with the Saccharomyces cerevisiae NTH1 gene. Multiple alignment of the predicted trehalase protein sequences from yeasts, bacteria, insects, and...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050436
更新日期:1997-04-01 00:00:00
abstract::Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Cal...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413014
更新日期:1984-06-01 00:00:00
abstract::It was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus Frankia. Recently, the number of nitrogen fixation (nifH) genes identified in other non-Frankia actinobacteria has dramatically increased and has open...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-011-0733-6
更新日期:2012-01-01 00:00:00
abstract::The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050505
更新日期:1997-10-01 00:00:00
abstract::Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409714
更新日期:1988-05-01 00:00:00
abstract::The acidic amino acids (Asp, Glu) and their amides (Asn, Gln) support rapid growth of a variety of Pseudomonas strains when provided as the sole source of carbon and nitrogen. All key enzymes of glutamate metabolism were detected in P. fluorescence, with glutaminase and asparaginase showing the highest specific activi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0511-6
更新日期:2003-03-01 00:00:00
abstract::In this study, Sorangium cellulosum So ce56 was phenotypically and genotypically analysed in order to evaluate whether this strain can be used in a comprehensive genome project as a representative of the secondary metabolite-producing myxobacteria. In contrast to many other strains of S. cellulosum, strain So ce56 was...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0479-2
更新日期:2002-12-01 00:00:00
abstract::Cadaverin was more readily incorporated than lysine into arthrobactin from Arthrobacter pascens and into ferrioxamin E from Streptomyces glaucescens. From a racemic mixture only the L-isomer of lysine is incorporated. The L-lysine decarboxylase activity was measured in vivo and in vitro. The enzyme from Arthrobacter p...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402309
更新日期:1978-05-30 00:00:00
abstract::The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0276-z
更新日期:2007-12-01 00:00:00
abstract::Elevation gradients, often regarded as "natural experiments or laboratories", can be used to study changes in the distribution of microbial diversity related to changes in environmental conditions that typically occur over small geographical scales. We exploited this feature by characterizing fungal composition and di...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01948-2
更新日期:2020-10-01 00:00:00
abstract::The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-018-1493-3
更新日期:2018-08-01 00:00:00
abstract::Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01805-x
更新日期:2020-07-01 00:00:00
abstract::VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1069-9
更新日期:2015-04-01 00:00:00
abstract::Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000160
更新日期:2000-05-01 00:00:00
abstract::Many bacterial cells are known to regulate their cooperative behaviors and physiological processes through a molecular mechanism called quorum sensing. Quorum sensing in Serratia marcescens JG is mediated by the synthesis of autoinducer 2 (AI-2) which is a furanosyl borate diester. In this study, the response of quoru...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1213-9
更新日期:2016-08-01 00:00:00
abstract::Given the serious threat of foodborne multidrug-resistant bacteria to animals and humans, finding an effective antibacterial compound has always been an important topic for scientists. Here, from the soil of Changbaishan, we have identified a bacterium that can inhibit the growth of Staphylococcus aureus. Nr genome da...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02102-8
更新日期:2020-11-04 00:00:00
abstract::Bacillus stearothermophilus strains PV 72 and ATCC 12980 carry a crystalline surface layer (S-layer) with hexagonal (p6) and oblique (p2) symmetry, respectively. Sites of insertions of new subunits into the regular lattice during cell growth have been determined by the indirect fluorescent antibody technique and the p...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446749
更新日期:1988-01-01 00:00:00