Localized insertion of new S-layer during growth of Bacillus stearothermophilus strains.

Abstract:

:Bacillus stearothermophilus strains PV 72 and ATCC 12980 carry a crystalline surface layer (S-layer) with hexagonal (p6) and oblique (p2) symmetry, respectively. Sites of insertions of new subunits into the regular lattice during cell growth have been determined by the indirect fluorescent antibody technique and the protein A/colloidal gold technique. During S-layer growth on both bacillus strains the following common features were noted: 1. shedding of intact S-layer or turnover of individual subunits was not seen; 2. new S-layer was deposited in helically-arranged bands over the cylindrical surface of the cell at a pitch angle related to the orientation of the lattice vectors of the crystalline array; 3. little or no S-layer was inserted into pre-existing S-layer at the poles, and 4. septal regions and, subsequently, newly formed cell poles were covered with new S-layer protein.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Gruber K,Sleytr UB

doi

10.1007/BF00446749

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

485-91

issue

6

eissn

0302-8933

issn

1432-072X

journal_volume

149

pub_type

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