Abstract:
:Bacillus stearothermophilus strains PV 72 and ATCC 12980 carry a crystalline surface layer (S-layer) with hexagonal (p6) and oblique (p2) symmetry, respectively. Sites of insertions of new subunits into the regular lattice during cell growth have been determined by the indirect fluorescent antibody technique and the protein A/colloidal gold technique. During S-layer growth on both bacillus strains the following common features were noted: 1. shedding of intact S-layer or turnover of individual subunits was not seen; 2. new S-layer was deposited in helically-arranged bands over the cylindrical surface of the cell at a pitch angle related to the orientation of the lattice vectors of the crystalline array; 3. little or no S-layer was inserted into pre-existing S-layer at the poles, and 4. septal regions and, subsequently, newly formed cell poles were covered with new S-layer protein.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Gruber K,Sleytr UBdoi
10.1007/BF00446749subject
Has Abstractpub_date
1988-01-01 00:00:00pages
485-91issue
6eissn
0302-8933issn
1432-072Xjournal_volume
149pub_type
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