Abstract:
:Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the maximal growth rate. However, maximal sigmaB activity was only reached when the growth stopped as a result of the exhaustion of the carbon and energy source glucose. During glucose-limited growth, increased expression of the general stress regulon at growth rates below 0.2 h-1 did not provide wild-type cells with a growth advantage over sigB mutants. Instead, expression of the stress regulon seems to constitute a significant burden during glucose-limited growth, resulting in a selective growth advantage of the sigB mutant as compared to the wild-type at a growth rate of 0.08 h-1.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Schweder T,Kolyschkow A,Völker U,Hecker Mdoi
10.1007/s002030050731keywords:
subject
Has Abstractpub_date
1999-05-01 00:00:00pages
439-43issue
6eissn
0302-8933issn
1432-072Xpii
91710439.203journal_volume
171pub_type
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