Abstract:
:We report structural and functional analyses of the Bradyrhizobium japonicum fixGHIS genes, which map immediately downstream of the fixNOQP operon for the symbiotically essential cbb3-type heme-copper oxidase complex. Expression of fixGHIS, like that of fixNOQP, is strongly induced in cells grown microaerobically or anaerobically. A fixGHI deletion led to the same prominent phenotypes as those known from a fixNOQP deletion: defective symbiotic nitrogen fixation (Fix-) and decreased cytochrome oxidase activity in cells grown under oxygen deprivation. Only traces, if any, of cytochrome cbb3 subunits were present in membranes isolated from the delta fixGHI strain, as revealed by Western blot analysis with subunit-specific antibodies. This effect was not due to lack of fixNOQP transcription. The results suggested a critical involvement of the fixGHIS gene products in the assembly and/or stability of the cbb3-type heme-copper oxidase. On the basis of sequence similarities between the FixI protein and a Cu-transporting P-type ATPase (CopA) of Enterococcus hirae, and between FixG and a membrane-bound oxidoreductase (RdxA) of Rhodobacter sphaeroides, we postulate that a membrane-bound FixGHIS complex might play a role in uptake and metabolism of copper required for the cbb3-type heme-copper oxidase.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Preisig O,Zufferey R,Hennecke Hdoi
10.1007/s002030050330subject
Has Abstractpub_date
1996-05-01 00:00:00pages
297-305issue
5eissn
0302-8933issn
1432-072Xjournal_volume
165pub_type
杂志文章abstract::Exogenous addition of adenosine- and guanosine 5'-(di- and tri) phosphate 3'-diphosphate (pppApp, ppApp, pppGpp and ppGpp) at the concentration of 0.5 mM inhibits spore germination of Streptomyces galilaeus ATCC 31133. This reversible inhibitory effect appeared to be at the transcriptional level, and also depends on t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446889
更新日期:1980-04-01 00:00:00
abstract::In an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16S rDNA sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. All except one st...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050350
更新日期:1996-07-01 00:00:00
abstract::Sulfolane (tetrahydrothiophene-1,1-dioxide) is used in the Sulfinol process for natural gas sweetening. At many sour-gas processing plants spills, landfills and leakage from unlined surface storage ponds have contaminated groundwaters with sulfolane. Due to its high water solubility and mobility in aquifers, sulfolane...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000184
更新日期:2000-07-01 00:00:00
abstract::Expression of the gene encoding ribosome modulation factor (RMF), as measured using an rmf-lacZ gene fusion, increased with decreasing pH in exponential phase cultures of Escherichia coli. Expression was inversely proportional to the growth rate and independent of the acidifying agent used and it was concluded that ex...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0025-0
更新日期:2005-10-01 00:00:00
abstract::Nodules collected from Alnus nepalensis growing in mixed forest stands at three different sites around Shillong, were crushed in various culture media to obtain isolates of Frankia. The isolates were found to have typical Frankia morphology as revealed by the scanning electron microscope. Seedlings inoculated with iso...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00276476
更新日期:1994-01-01 00:00:00
abstract::Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0686-0
更新日期:2004-10-01 00:00:00
abstract::The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA syn...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446529
更新日期:1975-12-31 00:00:00
abstract::The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the re...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0301-2
更新日期:2008-02-01 00:00:00
abstract::The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425807
更新日期:1984-01-01 00:00:00
abstract::We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0339-1
更新日期:2008-05-01 00:00:00
abstract::Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0457-z
更新日期:2009-04-01 00:00:00
abstract::Penicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0533-8
更新日期:2003-05-01 00:00:00
abstract::A Gram-reaction-negative, facultatively anaerobic bacterium, designated SAP-19T, was isolated from sap extracted from Acer pictum in Mt. Halla in Jeju, Republic of Korea and its taxonomic statue was investigated by a polyphasic approach including genome- and 16S rRNA gene-based phylogenetic analyses. Cells were motile...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01961-5
更新日期:2020-11-01 00:00:00
abstract::The coloration of cells of the cyanobacterium Synechococcus sp. PCC 7002 changed from normal blue-green to yellow-green when cells were grown at 15 degrees C in a medium containing nitrate as the sole nitrogen source. This change of coloration was similar to a general response to nutrient deprivation (chlorosis). For ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050535
更新日期:1998-01-01 00:00:00
abstract::A neutral protease (npr) (designated Bae16) toxic to nematodes was purified to homogeneity from the strain Bacillus nematocida. The purified protease showed a molecular mass of approximately 40 kDa and displayed optimal activity at 55 degrees C, pH 6.5. Bioassay experiments demonstrated that this purified protease cou...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0112-x
更新日期:2006-06-01 00:00:00
abstract::Glyoxalase I is a ubiquitous enzyme that detoxifies methylglyoxal, which is derived from glycolysis but inhibits the growth of cells from microorganisms to mammals. Here, the structural gene for glyoxalase I ( glo1(+)) from the fission yeast Schizosaccharomyces pombe was identified. Disruption of glo1(+) enhanced susc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0666-4
更新日期:2004-05-01 00:00:00
abstract::Tetrahydrofolate was shown to function as a methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate in cell extracts of the sulfate-reducing bacterium strain WN. Dimethylsulfoniopropionate-dependent activities were 0.56 micromol methyltetrahydrofolate min-1 (mg protein)-1 a...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050545
更新日期:1998-01-01 00:00:00
abstract::All Serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. DNA fragments of the two hemolysin genes hybridized with the chromosomal DNA of S. marcescens, S. liquefaciens, S. kiliensis, S. grimesii, S. proteamaculans, S. plym...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248958
更新日期:1990-01-01 00:00:00
abstract::The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. T...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0794-1
更新日期:2012-07-01 00:00:00
abstract::In beta-lactam-antibiotic-producing fungi, such as Aspergillus (Emericella) nidulans, L-alpha-aminoadipic acid is the branching point of the lysine and penicillin biosynthesis pathways. To obtain a deeper insight into the regulation of lysine biosynthesis genes, the regulation of the A. nidulans lysF gene, which encod...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000246
更新日期:2001-02-01 00:00:00
abstract::Dunaliella tertiolecta (marine) and D. viridis (halophilic) were each trained by serial transfer to grow at salt concentrations previously regarded as the other's domain. D. viridis then had a salt optimum at 1.0-1.5 M sodium chloride whereas that for D. tertiolecta was less than 0-2 M. Nevertheless D. tertiolecta gre...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00428592
更新日期:1977-05-13 00:00:00
abstract::Lactic acid bacteria can be considered as natural biopreservative and good biotechnological alternative to food safety. In this study, the antilisterial compounds produced by Enterococcus isolates from the Patagonian environment and their effectiveness for the control of Listeria monocytogenes in a food model were stu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02118-0
更新日期:2021-01-02 00:00:00
abstract::Purple sulfur bacterium Thiocapsa roseopersicina strain BBS requiring vitamin B12 may grow in the dark in media containing no other organic compounds. Under such conditions the cells oxidize sulfide and thiosulfate with the use of O2 and assimilate carbon dioxide. After 10--30s assimilation of NaH14CO3 about 60% of ra...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454854
更新日期:1976-07-01 00:00:00
abstract::Upland rice is an ecotype crop resulting from the long-term domestication and evolution of rice in dry land without a water layer. Generally, the stems and leaves are thick and luxuriant, while the leaves also typically broad and light. The root system is developed with abundant root hair, and the osmotic pressure of ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02058-9
更新日期:2020-09-29 00:00:00
abstract::Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatme...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245356
更新日期:1991-01-01 00:00:00
abstract::Both the changes in the activities of nitrogenase, glutamine synthetase and glutamate dehydrogenase and in the extracellular and intracellular NH4+ concentrations were investigated during the transition from an NH4+ free medium to one containing NH4+ ions for a continuous culture of Azotobacter vinelandii. If added in...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447319
更新日期:1975-06-22 00:00:00
abstract::Acanthamoeba castellanii is a free-living amoeba found mainly in humid environments and Arcobacter butzleri is an emerging zoonotic pathogen, both can establish in vitro endosymbiotic relationships in the absence of bacterial replication. We analyzed the localization of A. butzleri within A. castellanii establishing t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01699-9
更新日期:2019-12-01 00:00:00
abstract::Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1247-z
更新日期:2016-11-01 00:00:00
abstract::Xanthan-deficient mutants of Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, were generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, GumD. Mutants of gumD did not produce xanthan and remained pathogenic in citrus pl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0227-8
更新日期:2007-08-01 00:00:00
abstract::Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00412799
更新日期:1986-01-01 00:00:00