Abstract：
:Acanthamoeba castellanii is a free-living amoeba found mainly in humid environments and Arcobacter butzleri is an emerging zoonotic pathogen, both can establish in vitro endosymbiotic relationships in the absence of bacterial replication. We analyzed the localization of A. butzleri within A. castellanii establishing their association with endoplasmic reticulum vesicles and mitochondria. Through confocal microscopy, we observed that during the early stages of endosymbiosis, there is not colocalization between amoebic vacuoles containing A. butzleri and mitochondria or ER vesicles of A. castellanii. Considering that energy production of this bacterium occurs via metabolism of amino acids or the tricarboxylic acid cycle, these results contribute to explain the absence of bacterial replication, since A. butzleri would not have access to the nutrients found in endoplasmic reticulum vesicles and mitochondria. In addition, we observe that A. butzleri induces significantly the actin polymerization of A. castellanii during the early stages of endosymbiosis.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Medina G,Leyán P,da Silva CV,Flores-Martin S,Manosalva C,Fernández Hdoi
10.1007/s00203-019-01699-9subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1447-1452issue
10eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-019-01699-9journal_volume
201pub_type
杂志文章abstract：:Pulicat Lake sediments are often severely polluted with the toxic heavy metal mercury. Several mercury-resistant strains of Bacillus species were isolated from the sediments and all the isolates exhibited broad spectrum resistance (resistance to both organic and inorganic mercuric compounds). Plasmid curing assay show...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0088-6
更新日期：2006-04-01 00:00:00
abstract：:We explored the molecular diversity of cytochrome P450 genes in the filamentous fungus Aspergillus oryzae using bioinformatic and experimental approaches. Based on bioinformatic annotation, we found 155 putative genes of cytochromes P450 in the whole genome sequence; however, 13 of 155 appeared to be pseudogenes due t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0562-z
更新日期：2010-05-01 00:00:00
abstract：:The anaerobic fungus Piromyces sp. strain E2 produces extracellular cellulolytic enzymes present both in a high molecular mass (HMM) complex or as individual proteins. Although the HMM complex was present in the culture fluid during all growth stages, the highest amounts of complex were obtained when cultures were har...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:The objective of this work was to investigate the structure and diversity of lactic acid bacteria (LAB) communities in traditionally fermented meat collected from different areas of Tunisia. A polyphasic study, which involves phenotypic tests and ribosomal DNA-based techniques, was used to identify Gram-positive and c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0499-2
更新日期：2009-09-01 00:00:00
abstract：:A novel thermotolerant strain of the achlorophyllous micro-alga Prototheca was isolated from a hot spring. The isolate was found to produce an appreciable amount of ethanol and CO2 from glucose under anoxic conditions at both 25 and 40 degrees C; this type of alcohol fermentation has not yet been reported in the genus...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0384-0
更新日期：2002-03-01 00:00:00
abstract：:The essential oil (EO) from the roots of Plectranthus barbatus Andr. (Syn. Coleus forskohlii Briq.) was evaluated for quorum sensing (QS) inhibitory activity. P. barbatus EO was screened for inhibition of QS regulated violacein production in Chromobacterium violaceum (ATCC 12472) wild-type strain. At inhibitory (6.25%...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02171-9
更新日期：2021-01-20 00:00:00
abstract：:All Serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. DNA fragments of the two hemolysin genes hybridized with the chromosomal DNA of S. marcescens, S. liquefaciens, S. kiliensis, S. grimesii, S. proteamaculans, S. plym...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248958
更新日期：1990-01-01 00:00:00
abstract：:Ostracods collected from shallow coral reefs in the Bahamas were found to exhibit blue light-stimulated orange fluorescence at night. Fluorescent spectra revealed the presence of orange fluorescence with a maximum emission at ~595 nm on the carapace of these ostracods, while scanning electron microscopy revealed a mor...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0911-9
更新日期：2013-09-01 00:00:00
abstract：:Hopane glycolipid, isolated from Bacillus acidocaldarius, was studied in its ability to support growth from Mycoplasma mycoides var. capri as substitute for sterols. All attempts to culture Mycoplasma on hopane glycolipid failed. When added together with cholesterol, the effect of HGL was a reduction in growth rate. T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413519
更新日期：1982-12-01 00:00:00
abstract：:Milk containing antibiotics is used as cost-effective feed for calves, which may lead to antibiotic residues-associated food safety problems. This study aims to investigate the influence of antibiotics on rumen microbes. Through metagenomic sequencing, the rumen microbial communities of calves fed with pasteurized mil...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1311-8
更新日期：2017-04-01 00:00:00
abstract：:It has been shown previously that externally added glycine betaine is accumulated in Escherichia coli in response to the external osmotic strength. Here we have shown, by using nuclear magnetic resonance spectroscopy and radiochemical methods, that E. coli growing in a glucose-mineral medium of elevated osmotic streng...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492896
更新日期：1987-02-01 00:00:00
abstract：:The free tryptophan pool and the levels of two enzymes of tryptophan biosynthesis (anthranilate synthase and indoleglycerolphosphate synthase)have been determined in a wild type strain of Saccharomyces cerevisiae and in mutants with altered regulatory properties. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446842
更新日期：1976-03-19 00:00:00
abstract：:Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01805-x
更新日期：2020-07-01 00:00:00
abstract：:Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhib...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410896
更新日期：1984-08-01 00:00:00
abstract：:Effects of different flavonoids on various bacterial strains have been extensively reported; however, the mechanism(s) of their action on bacterial cells remain largely elusive. In this study, the antibacterial mechanism of soybean isoflavone (SI) on Staphylococcus aureus is systematically investigated using 4'6-diami...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0617-1
更新日期：2010-11-01 00:00:00
abstract：:The signal orchestration between legumes and the rhizobia attribute to symbiotic nitrogen fixation through nodule formation. Root nodules serve as a nutrient-rich reservoir and harbor diverse microbial communities. However, the existence of non-rhizobial endophytes (NRE) and their role inside the root nodules are bein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01983-z
更新日期：2020-12-01 00:00:00
abstract：:Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1166-4
更新日期：2016-03-01 00:00:00
abstract：:Cells of the yeast Saccharomycopsis capsularis fused in pairs after disolving of part of the cross wall between them near the lateral wall. After nuclear migrations through the opening, the cross wall was closed again and the cells at both sides became asci. The wall of the ascospores developed from a prospore wall. B...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447335
更新日期：1975-08-28 00:00:00
abstract：:Two styrene monooxygenase types, StyA/StyB and StyA1/StyA2B, have been described each consisting of an epoxidase and a reductase. A gene fusion which led to the chimeric reductase StyA2B and the occurrence in different phyla are major differences. Identification of SMOA/SMOB-ADP1 of Acinetobacter baylyi ADP1 may enlig...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1022-y
更新日期：2014-12-01 00:00:00
abstract：:The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleyl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00276301
更新日期：1992-01-01 00:00:00
abstract：:Video-enhanced light microscopy of the apical and subapical regions of growing hyphae of several fungal species revealed the existence of momentary synchronized motions of subcellular organelles. First discovered in a temperature-sensitive morphological mutant (ramosa-1) of Aspergillus niger, these seemingly spontaneo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0771-z
更新日期：2005-05-01 00:00:00
abstract：:Monoclonal antibodies have been used to determine the presence of cellobiohydrolases I and II (CBH I and II), and endoglucanase I (EG I) on the surface of conidia from Trichoderma reesei QM 9414 and RUT C-30, and 8 other Trichoderma species. For this purpose, proteins were released from the conidial surface by treatme...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245356
更新日期：1991-01-01 00:00:00
abstract：:Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0414-2
更新日期：2008-12-01 00:00:00
abstract：:The molar percentages of guanine plus cytosine in the DNA of 51 strains of Simonsiellaceae were determined by buoyant density ultracentrifugation of cell lysates in CsCl. The DNA base ratios ranged from 41-55 mole-% guanine plus cytosine. These values fall within the range known for the Order Cytophagales, the non-fru...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492026
更新日期：1977-06-20 00:00:00
abstract：:The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406155
更新日期：1980-12-01 00:00:00
abstract：:Dunaliella tertiolecta (marine) and D. viridis (halophilic) were each trained by serial transfer to grow at salt concentrations previously regarded as the other's domain. D. viridis then had a salt optimum at 1.0-1.5 M sodium chloride whereas that for D. tertiolecta was less than 0-2 M. Nevertheless D. tertiolecta gre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428592
更新日期：1977-05-13 00:00:00
abstract：:Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm ( Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0543-6
更新日期：2003-05-01 00:00:00
abstract：:1. Mutants derived from the hydrogen bacterium Alcaligenes eutrophus strain H 16 auxotrophic for phenylalanine and tyrosine were isolated employing mutagenic agents (EMS, nitrite), the colistine counterselection technique and the "pin-point" isolation method. Three different types of mutants were found: (1) Mutants, r...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436341
更新日期：1975-04-07 00:00:00
abstract：:Colistin is a mixture of polymyxin E1 and E2, bactericidal pentacationic lipopeptides used to treat infections caused by Gram-negative pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae. Industrial production of colistin is obtained by a fermentation process of the natural producer Paenibacillus polymy...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1084-5
更新日期：2015-05-01 00:00:00
abstract：:Cadaverin was more readily incorporated than lysine into arthrobactin from Arthrobacter pascens and into ferrioxamin E from Streptomyces glaucescens. From a racemic mixture only the L-isomer of lysine is incorporated. The L-lysine decarboxylase activity was measured in vivo and in vitro. The enzyme from Arthrobacter p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402309
更新日期：1978-05-30 00:00:00
