Abstract:
:A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-7.8 and 34-38 degrees C. The organism grew with L-lactate, pyruvate, butyrate, formate, succinate, or ethanol as electron donors, using either tetrachloroethene, 2-chlorophenol, 2,4,6-trichlorophenol, 3-chloro-4-hydroxy-phenylacetate, sulfite, thiosulfate, or fumarate as electron acceptors. Strain PCE1 also grew fermentatively with pyruvate as the sole substrate. L-Lactate and pyruvate were oxidized to acetate. Tetrachloroethene was reductively dechlorinated to trichloroethene and small amounts ( 5%) of cis-1,2-dichloroethene and trans-1,2-dichloroethene. Chlorinated phenolic compounds were dechlorinated specifically at the ortho-position. On the basis of 16S rRNA sequence analysis, the organism was identified as a species within the genus Desulfitobacterium, which until now only contained the chlorophenol-dechlorinating bacterium, Desulfitobacterium dehalogenans.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Gerritse J,Renard V,Pedro Gomes TM,Lawson PA,Collins MD,Gottschal JCdoi
10.1007/s002030050308subject
Has Abstractpub_date
1996-02-01 00:00:00pages
132-40issue
2eissn
0302-8933issn
1432-072Xjournal_volume
165pub_type
杂志文章abstract::Chloroquine inhibited the outgrowth of Bacillus subtilis spores at a 10-fold lower concentration than that required to prevent vegetative growth. Analysis of macromolecular synthesis in outgrowing spores and vegetative cells in the presence of chloroquine indicated that it acted preferentially on transcription. Differ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409659
更新日期:1989-01-01 00:00:00
abstract::Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050589
更新日期:1998-05-01 00:00:00
abstract::An unidentified filamentous purple bacterium, probably belonging to a new genus or even a new family, is found in close association with the filamentous, mat-forming cyanobacterium Microcoleus chthonoplastes in a hypersaline pond at Guerrero Negro, Baja California Sur, Mexico, and in Solar Lake, Sinai, Egypt. This or...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00463477
更新日期:1987-01-01 00:00:00
abstract::Inactivation of peroxisomal enzymes in the yeast Hansenula polymorpha was studied following transfer of cells into cultivation media in which their activity was no longer required for growth. After transfer of methanol-grown cells into media containing glucose - a substrate that fully represses alcohol oxidase synthes...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00407757
更新日期:1983-06-01 00:00:00
abstract::The twin-arginine translocation (Tat) system serves to export fully folded proteins across the cytoplasmic membrane. In many bacteria, three major components, TatA, TatB and TatC, are the functionally essential constituents of the Tat system. A Myxococcus xanthus tatB-tatC deletion mutant could aggregate and form moun...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0067-3
更新日期:2006-02-01 00:00:00
abstract::Penicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0533-8
更新日期:2003-05-01 00:00:00
abstract::Serine (Ser)/threonine (Thr) or tyrosine (Tyr) protein kinases in eukaryotes contain RDxKxxN or RDx(A/R)A(A/R)N sequences, respectively, in the catalytic loop. Myxococcus xanthus DspB is a dual-specificity kinase that contains an atypical sequence, RDVAQKN, in the catalytic loop. The DspB mutant (A165K), which contain...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1181-5
更新日期:2016-04-01 00:00:00
abstract::The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245305
更新日期:1993-01-01 00:00:00
abstract::Hopane glycolipid, isolated from Bacillus acidocaldarius, was studied in its ability to support growth from Mycoplasma mycoides var. capri as substitute for sterols. All attempts to culture Mycoplasma on hopane glycolipid failed. When added together with cholesterol, the effect of HGL was a reduction in growth rate. T...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413519
更新日期:1982-12-01 00:00:00
abstract::The isolated cell wall of Streptomyces griseus 52-1 strain labelled with fluorescein isothiocyanate (FITC) and containing wall-bound autolytic enzyme was lysed as a function of different cations. The autolysis was accelerated by aminoglycoside antibiotics (streptomycin and the structurally closely related neomycin) wh...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00291282
更新日期:1990-01-01 00:00:00
abstract::In Lactobacillus casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L. casei and shown to be clustered with the gene encoding pyruvate kinase (pyk). pfk and pyk ge...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0003-6
更新日期:2005-09-01 00:00:00
abstract::Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytopl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425171
更新日期:1989-01-01 00:00:00
abstract::Expression of unnecessary proteins is known to reduce the growth rate of Escherichia coli. This reduction in growth rate, due to the diversion of cellular resources from making essential proteins, is called cost. Cellular resources depend upon the macromolecular content of the cell. Cost phenomenon lies in the process...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0967-1
更新日期:2014-04-01 00:00:00
abstract::Studying the effects of microbial volatile organic compounds (VOCs) on plant growth is challenging because the production of volatiles depends on many environmental factors. Adding to this complexity, the method of volatile exposure itself can lead to different responses in plants and may account for some of the contr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1104-5
更新日期:2015-06-01 00:00:00
abstract::Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0457-z
更新日期:2009-04-01 00:00:00
abstract::The cold shock response in bacteria involves the expression of low-molecular weight cold shock proteins (CSPs) containing a nucleic acid-binding cold shock domain (CSD), which are known to destabilize secondary structures on mRNAs, facilitating translation at low temperatures. Caulobacter crescentus cspA and cspB are ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0602-8
更新日期:2010-09-01 00:00:00
abstract::The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0865-3
更新日期:2013-03-01 00:00:00
abstract::Effects of different flavonoids on various bacterial strains have been extensively reported; however, the mechanism(s) of their action on bacterial cells remain largely elusive. In this study, the antibacterial mechanism of soybean isoflavone (SI) on Staphylococcus aureus is systematically investigated using 4'6-diami...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0617-1
更新日期:2010-11-01 00:00:00
abstract::The starvation survivability of seven Thermococcus strains isolated from four Japanese oil reservoirs was compared with that of Thermococcus strains from marine hydrothermal fields. 16S rDNA analyses showed the isolates to be closely related to Thermococcus litoralis. Growth of the isolates was dependent on amino acid...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100318
更新日期:2001-10-01 00:00:00
abstract::A novel coccoid-shaped, hyperthermophilic, heterotrophic member of the archaea was isolated from a shallow marine hydrothermal system at Vulcano Island, Italy. The isolate grew between 56 and 90 degrees C with an optimum around 85 degrees C. The pH range for growth was 6.5 to 10.5, with an optimum around 9.0. Polysulf...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of gram-positive bacteria other than Staphylococcus. CB added to the medium of growing cultures of strains of genus Micrococcus, Streptococcus, Lactobacillus and Bacillus...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248617
更新日期:1991-01-01 00:00:00
abstract::An organism from a goldfish aquarium, isolated on barbital medium, was found to be a Gram-positive coccus which divided in alternating planes, often appearing as a doublet or as a tetrad with adjacent sides flattened. It grew well, although slowly, on rich solid medium (LB agar) and in liquid brain-heart infusion at r...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00288712
更新日期:1993-01-01 00:00:00
abstract::A temperature-sensitive mutant of Salmonella typhimurium was isolated earlier after transposon mutagenesis with Tn10d Tet. The mutant D220 grows well at 28 degreesC but has a lower growth rate and forms filaments at 37 degreesC. Transposon-flanking fragments of mutant D220 DNA were cloned and sequenced. The transposon...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050607
更新日期:1998-06-01 00:00:00
abstract::The regulatory elements involved in expression of the gene (fdhF) for the selenopolypeptide of formate dehydrogenase and of a gene (or transcriptional unit) (hyd) specifically responsible for the formation of the gas-evolving hydrogenase (hydrogenase 3) in Escherichia coli were investigated. Formate (or a product of i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429646
更新日期:1987-06-01 00:00:00
abstract::Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0848-4
更新日期:2013-01-01 00:00:00
abstract::1. Mutants derived from the hydrogen bacterium Alcaligenes eutrophus strain H 16 auxotrophic for phenylalanine and tyrosine were isolated employing mutagenic agents (EMS, nitrite), the colistine counterselection technique and the "pin-point" isolation method. Three different types of mutants were found: (1) Mutants, r...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00436341
更新日期:1975-04-07 00:00:00
abstract::Succinate:quinone reductase is a membrane-bound enzyme of the citric acid cycle and the respiratory chain. Carboxin is a potent inhibitor of the enzyme of certain organisms. The bacterium Paracoccus denitrificans was found to be sensitive to carboxin in vivo, and mutants that grow in the presence of 3'-methyl carboxin...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050611
更新日期:1998-07-01 00:00:00
abstract::Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 degrees C in EDTA-free medium. High-molecular-weight mannop...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00307769
更新日期:1994-01-01 00:00:00
abstract::In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1051-6
更新日期:2015-03-01 00:00:00
abstract::Synechococcus elongatus strain PCC7942 cells were grown in high or low environmental concentrations of inorganic C (high-C(i), low-C(i)) and subjected to a light shift from 50 micromol m(-2) s(-1) to 500 micromol m(-2) s(-1). We quantified photosynthetic reductant (O(2) evolution) and molar cellular contents of phycob...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0761-1
更新日期:2005-03-01 00:00:00