Repression of toxin production by tryptophan in Clostridium botulinum type E.

Abstract:

:Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum type E. On the other hand, the addition of an enzymatic digest of casein (NZ Case) stimulated toxin formation and overcame repression by tryptophan. Immunoblots of proteins in culture fluids using antibodies to type E toxin indicated that tryptophan-repressed cultures produced less neurotoxin protein. Inhibitors of neurotoxin did not accumulate in cultures grown in minimal medium supplemented with high tryptophan. The results suggest that tryptophan availability in foods or in the intestine may be important for toxin formation by C. botulinum type E.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Leyer GJ,Johnson EA

doi

10.1007/BF00245225

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

443-7

issue

5

eissn

0302-8933

issn

1432-072X

journal_volume

154

pub_type

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