Specificity of translation of spore polypeptides in bacillus in vitro systems.

Abstract:

:Cell free extracts prepared from exponentially growing Escherichia coli and Bacillus cereus as well as from Bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with Bacillus ribonucleic acid. All three preparations synthesized glutamine synthetase antigen when ribonucleic acid from a Bacillus subtilis strain that overproduces glutamine synthetase was added. The post exponential Bacillus cereus extract, however, was most active for the synthesis of Bacillus cereus sport coat antigen when supplemented with the appropriate ribonucleic acid. There appears to be some specificity in the translation of at least this sporulation messenger RNA.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Wright W 3rd,Aronson AI

doi

10.1007/BF00455354

subject

Has Abstract

pub_date

1981-04-01 00:00:00

pages

160-4

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

129

pub_type

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