Abstract：
:In autotrophic methanogens, pyruvate oxidoreductase (POR) plays a key role in the assimilation of CO(2) and the biosynthesis of organic carbon. This enzyme has been purified to homogeneity, and the genes from Methanococcus maripaludis were sequenced. The purified POR contained five polypeptides with molecular masses of 47, 33, 25, 21.5 and 13 kDa. The N-terminal sequences of four of the polypeptides had high similarity to the subunits commonly associated with this enzyme from other archaea. However, the 21.5-kDa polypeptide had not been previously observed in PORs. Nucleotide sequencing of the gene cluster encoding the POR revealed six open reading frames ( porABCDEF). The genes porABCD corresponded to the subunits previously identified in PORs. On the basis of the N-terminal amino acid sequence, porE encoded the 21.5-kDa polypeptide and contained a high cysteinyl residue content and a motif indicative of a [Fe-S] cluster. porF also had a high sequence similarity to porE, a high cysteinyl residue content, and two [Fe-S] cluster motifs. Homologs to porE were also present in the genomic sequences of the autotrophic methanogens Methanocaldococcus jannaschii and Methanothermobacter thermautotrophicus. Based upon these results, it is proposed that PorE and PorF are components of a specialized system required to transfer low-potential electrons for pyruvate biosynthesis. Some biochemical properties of the purified methanococcal POR were also determined. This unstable enzyme was very sensitive to O(2 )and demonstrated high activity with pyruvate, oxaloacetate, and alpha-ketobutyrate. Methyl viologen, rubredoxin, FMN, and FAD were readily reduced. Activity was also observed with spinach and clostridial ferredoxins and cytochrome c. Coenzyme F(420) was not an electron acceptor for the purified enzyme.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Lin WC,Yang YL,Whitman WBdoi
10.1007/s00203-003-0554-3keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
444-56issue
6eissn
0302-8933issn
1432-072Xjournal_volume
179pub_type
杂志文章abstract：:The occurrence of emodin, erythroglaucin, physcion, physcion-9-anthrone, questin, catenarin, and catenarin-8-methyl ether in different species of the Aspergillus glaucus group (genus Eurotium) was investigated. So far catenarin-8-methyl ether (1, 4, 6-trihydroxy-8-methoxy-3-methylanthraquinone) has not been described ...
journal_title：Archives of microbiology
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更新日期：2015-06-01 00:00:00
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journal_title：Archives of microbiology
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更新日期：1994-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425099
更新日期：1976-05-03 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1976-02-01 00:00:00
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：1998-03-01 00:00:00
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更新日期：2008-03-01 00:00:00
