Abstract：
:NAD-linked lactate dehydrogenases specific for the D- and L-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied. The enzyme has a molecular weight of ca. 115000-120000, is highly specific, autooxidizable, and susceptible to inhibition by iodoacetamide, oxamate and ATP. The possible physiological functions of the enzyme in the metabolism of the organism were investigated. D-lactate carbon was incorporated in cell material during photosynthetic growth with CO2, but lactate was not used as sole source for carbon for photosynthetic or chemosynthetic development. D-lactate and pyruvate were oxidized aerobically in the dark by resting cell suspensions with the assimilation mainly of the C2 and the C3 carbon atoms. In the oxidation of lactate, acetate was excreted into the medium. No fermentation of glucose was found, but a small amount of D-lactate was detected as a product of endogenous dark metabolism of the cell. All enzymes required for the production of lactate from glucose and from glycogen were found in exponentially growing cells, but the activity of some key enzymes was low or undetectable in old cultures.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Sanchez JJ,Palleroni NJ,Doudoroff Mdoi
10.1007/BF00447300subject
Has Abstractpub_date
1975-06-20 00:00:00pages
57-65issue
1eissn
0302-8933issn
1432-072Xjournal_volume
104pub_type
杂志文章abstract：:A novel actinomycete, strain PA1-10T, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aer...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01717-w
更新日期：2020-01-01 00:00:00
abstract：:L-(+)-lactate dehydrogenase (LDH) from Staphylococcus epidermidis ATCC 14990 was purified by affinity chromatography. The purified enzyme was specifically activated by fructose-1,6-diphosphate (FDP). The concentration of FDP required for 50% maximal activity was about 0.15 mM. The enzyme activity was inhibited by aden...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447150
更新日期：1975-11-07 00:00:00
abstract：:The Streptomyces virginiae gamma-butyrolactone autoregulator virginiae butanolide is a low-molecular-weight Streptomyces hormone eliciting virginiamycin biosynthesis through its binding to the specific receptor protein, BarA. Immediately downstream of barA lies barB, the transcription of which is tightly repressed by ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0625-5
更新日期：2004-01-01 00:00:00
abstract：:The nitrite oxidoreductase (NOR) from the facultative nitrite-oxidizing bacterium Nitrobacter hamburgensis X14 was investigated genetically. In order to develop a probe for the gene norB, the N-terminal amino acid sequence of the NOR beta-subunit (NorB) was determined. Based on that amino acid sequence, an oligonucleo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
abstract：:Microbes from diverse types of habitats are continuously exposed to external challenges, which may include acidic, alkaline, and toxic metabolites stress as well as nutrient deficiencies. To promote their own survival, bacteria have to rapidly adapt to external perturbations by inducing particular stress responses tha...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-020-02050-3
更新日期：2020-09-25 00:00:00
abstract：:Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mas...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00262202
更新日期：1995-01-01 00:00:00
abstract：:The production of secondary metabolites with antibiotic properties is a common characteristic to Bacillus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities with medicinal and agricultural interests such as antibiotic. Bacillus sp. fmbJ has been found to produc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0939-x
更新日期：2014-02-01 00:00:00
abstract：:The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00521289
更新日期：1982-12-03 00:00:00
abstract：:We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombinati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409661
更新日期：1989-01-01 00:00:00
abstract：:The site of integration of phage M mu d (Ap lac) in mutant M9s which leads to deficiency of formic dehydrogenase (benzylviologen-linked) activity was determined. It was shown that the phage had inserted into the gene for the seleno-polypeptide of the enzyme (80 kd) leading to the formation of a truncated peptide (60 k...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428850
更新日期：1985-05-01 00:00:00
abstract：:The entomopathogenic mushroom Cordyceps militaris is a storehouse of various medicinal compounds and pharmacological effects. However, the high frequency of strain degeneration during subculture and preservation severely limits the large-scale production of C. militaris. DNA methylation is an important epigenomic modi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01621-3
更新日期：2019-04-01 00:00:00
abstract：:NAD kinase was purified 180-fold from Bacillus licheniformis to determine the role it plays in NADP turnover in this organism. The enzyme was found to have a pH optimum of 6.8 and an apparent Km for NAD of 2.7 mM. The ATP saturation curve was not hyperbolic; 5.5 mM ATP was required to reach half maximal activity. Both...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409878
更新日期：1986-05-01 00:00:00
abstract：:This study describes two Bradyrhizobium strains, UFLA03-164T and UFLA03-153, which share more than 99% sequence similarity of the 16S rRNA with the type strains of 15 species in this genus. The concatenation of three housekeeping genes (recA, gyrB, and dnaK) indicated that both strains formed a single clade separate f...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01827-w
更新日期：2020-07-01 00:00:00
abstract：:The sulfate-reducing highly enriched culture N47 is capable to anaerobically degrade naphthalene, 2-methylnaphthalene, and 2-naphthoic acid. A proteogenomic investigation was performed to elucidate the initial activation reaction of anaerobic naphthalene degradation. This lead to the identification of an alpha-subunit...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0667-4
更新日期：2011-04-01 00:00:00
abstract：:Tetrahydrofolate was shown to function as a methyl acceptor in the anaerobic demethylation of dimethylsulfoniopropionate to methylthiopropionate in cell extracts of the sulfate-reducing bacterium strain WN. Dimethylsulfoniopropionate-dependent activities were 0.56 micromol methyltetrahydrofolate min-1 (mg protein)-1 a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050545
更新日期：1998-01-01 00:00:00
abstract：:Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 degrees C in EDTA-free medium. High-molecular-weight mannop...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00307769
更新日期：1994-01-01 00:00:00
abstract：:During the development of fruit bodies of the basidiomycete Schizophyllum commune, the alkali-insoluble (R glucan) and alkali-soluble (S glucan) cell wall fractions are synthesized during the entire course of morphogenesis. The water soluble glucan (WSG) is not synthesized after an early stage. There is also a relativ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429623
更新日期：1977-07-26 00:00:00
abstract：:Chlorella fusca, strain 211-15, cells degreened in a nitrogen-deficient mineral growth medium in the light for 4-6 weeks were regreened for up to 24 hrs in a nitrogen rich medium that leads to synchronous cell division at 24-26 hrs. Structural changes in the plastid membranes during the regreening period were observed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436359
更新日期：1975-05-05 00:00:00
abstract：:Cyanothece sp. strain ATCC 51142 is an aerobic, unicellular, diazotrophic cyanobacterium that temporally separates O2-sensitive N2 fixation from oxygenic photosynthesis. The energy and reducing power needed for N2 fixation appears to be generated by an active respiratory apparatus that utilizes the contents of large i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced beta-oxidation pathway showed the presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase. After isopicnic centrifugation in a ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050392
更新日期：1996-11-01 00:00:00
abstract：:P1B-type ATPases are involved in heavy metal transport across the plasma membrane. Some Mycobacterium tuberculosis P-type ATPases are induced during infection, suggesting that this type of transporter could play a critical role in mycobacterial survival. To date, the ion specificity of M. tuberculosis heavy metal-tran...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1465-z
更新日期：2018-04-01 00:00:00
abstract：:The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01833-y
更新日期：2020-07-01 00:00:00
abstract：:Pyruvate kinase (ATP: pyruvate phosphotransferase (EC 2.7.1.40) was partially purified from both autotrophically and heterotrophically grown Paracoccus denitrificans. The organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. The enzyme isolated from b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427847
更新日期：1977-10-24 00:00:00
abstract：:Besides carotenoids a complex of flexirubin-type pigments was isolated from the gliding bacterium Cytophaga johnsonae Cy jl and separated into 6 components, which partly containe chlorine. In spite of the fact that these components still consist of pigment mixtures, the gross structures of 18 new flexirubin-type pigme...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00738543
更新日期：1978-06-26 00:00:00
abstract：:We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0339-1
更新日期：2008-05-01 00:00:00
abstract：:To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000236
更新日期：2001-01-01 00:00:00
abstract：:In yeasts, several sensing systems localized to the plasma membrane which transduce information regarding the availability and quality of nitrogen and carbon sources and work in parallel with the intracellular nutrient-sensing systems, regulate the expression and activity of proteins involved in nutrient uptake and ut...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0022-3
更新日期：2005-11-01 00:00:00
abstract：:Yeast suffers from a variety of environmental stresses, such as osmotic pressure and ethanol produced during fermentation. Since calcium ions are protective for high concentrations of ethanol, we investigated whether Ca(2+) flux occurs in response to ethanol stress. We find that exposure of yeast to ethanol induces a ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0673-6
更新日期：2011-05-01 00:00:00
abstract：:Polyhydroxyalkanoates (PHAs) are biological polyesters, of which, Short-Chain-Length-Medium-Chain-Length (SCL-MCL) PHA copolymers are important because of their wide range of applications. The present study focused on molecular characterization of Pseudomonas sp. LDC-5 that is identified as SCL-MCL producer. Phase con...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0265-2
更新日期：2007-11-01 00:00:00
abstract：:The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0412-8
更新日期：2002-06-01 00:00:00
