The heat shock response in the cyanobacterium Synechocystis sp. Strain PCC 6803 and regulation of gene expression by HrcA and SigB.

Abstract:

:We report on the genome-wide response, based on DNA microarrays, of the cyanobacterium Synechocystis sp. PCC 6803 wild type and DeltasigB to a 15 min heat shock. Approximately 9% of the genes in wild type and DeltasigB were significantly regulated (P < 0.001) following this treatment, with chaperones induced the most. The absence of sigB had no dramatic effect on specific genes induced by heat shock, but did affect the level of transcription of the chaperones. In addition, sigE was induced in DeltasigB. Comparison of global gene expression of the wild type and the hrcA mutant at 30 degrees C enabled us to examine the HrcA regulon, which included groESL and groEL2. Several genes belonging to specific functional groups (e.g., pilus biogenesis/assembly and phototaxis, biosynthesis of aromatic amino acids, murien sacculus and peptidoglycan, surface polysaccharides, and the Sec pathway) were differentially regulated following heat shock. We used results from knock-out mutants in sigB, sigD and sigE to construct a model of the network of group 2 sigma factor regulation upon each other. In this network, SigB represented the major node and SigE a secondary node. Overall, we determined that transcription of the heat-shock genes are regulated to various degrees by SigB, SigE and HrcA.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Singh AK,Summerfield TC,Li H,Sherman LA

doi

10.1007/s00203-006-0138-0

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

273-86

issue

4

eissn

0302-8933

issn

1432-072X

journal_volume

186

pub_type

杂志文章
  • Analysis of phenomena involved in the apical growth of Phycomyces blakesleeanus.

    abstract::The effects of the Ca(2+)/H(+) exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of Phycomyces blakesleeanus were analyzed. While all of the compou...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0609-5

    authors: Ruiz-Herrera J,Martínez-Cadena G,Loarca F,Salcedo-Hernández R

    更新日期:2003-12-01 00:00:00

  • Bacillus megaterium-induced biocorrosion on mild steel and the effect of Artemisia pallens methanolic extract as a natural corrosion inhibitor.

    abstract::Methanolic extract of Artemisia pallens (MEAP) (Asteraceae) was explored as greenbiocorrosion inhibitor for mild steel 1010 in 1.5% sodium chloride environment. Bacillus megaterium SKR7 induces the development of biofilm on the metal surface and forms the pitting corrosion. MEAP was showed (25 ppm) optimum inhibition ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-01951-7

    authors: Kokilaramani S,AlSalhi MS,Devanesan S,Narenkumar J,Rajasekar A,Govarthanan M

    更新日期:2020-10-01 00:00:00

  • Understanding the host-adapted state of Citrobacter rodentium by transcriptomic analysis.

    abstract::Citrobacter rodentium (Cr) is a mouse pathogen that mimics many aspects of enteropathogenic Escherichia coli infections including producing attaching and effacing (A/E) lesions. Host-adapted (HA) Cr cells that are shed at the peak of infection have been reported to be hyper-infective. The exact mechanism underlying th...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-016-1191-y

    authors: Smith AD,Yan X,Chen C,Dawson HD,Bhagwat AA

    更新日期:2016-05-01 00:00:00

  • Ultrastructural and chemotaxonomic analysis of a xylanolytic strain of Cryptococcus adeliensis isolated from sheep droppings in Spain.

    abstract::Cryptococcus adeliensis was initially described as a psycrophilic species containing a single strain CBS 8351(T) isolated from decayed algae in Terre Adelie (Antartida). Later, a second strain of this species was isolated from an immunosuppressed patient affected by leukaemia in Germany and recently several strains fr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-006-0134-4

    authors: Velázquez E,del Villar M,Grondona I,Monte E,González-Villa T

    更新日期:2006-09-01 00:00:00

  • The impact of cell structure, metabolism and group behavior for the survival of bacteria under stress conditions.

    abstract::Microbes from diverse types of habitats are continuously exposed to external challenges, which may include acidic, alkaline, and toxic metabolites stress as well as nutrient deficiencies. To promote their own survival, bacteria have to rapidly adapt to external perturbations by inducing particular stress responses tha...

    journal_title:Archives of microbiology

    pub_type: 杂志文章,评审

    doi:10.1007/s00203-020-02050-3

    authors: Zhang X,Li Z,Pang S,Jiang B,Yang Y,Duan Q,Zhu G

    更新日期:2020-09-25 00:00:00

  • The anabolic pyruvate oxidoreductase from Methanococcus maripaludis.

    abstract::In autotrophic methanogens, pyruvate oxidoreductase (POR) plays a key role in the assimilation of CO(2) and the biosynthesis of organic carbon. This enzyme has been purified to homogeneity, and the genes from Methanococcus maripaludis were sequenced. The purified POR contained five polypeptides with molecular masses o...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0554-3

    authors: Lin WC,Yang YL,Whitman WB

    更新日期:2003-06-01 00:00:00

  • The upstream region of the isocitrate lyase gene (UPR-ICL) of Candida tropicalis induces gene expression in both Saccharomyces cerevisiae and Escherichia coli by acetate via two distinct promoters.

    abstract::The upstream region of the isocitrate lyase gene (UPR-ICL, 1530bp) of an n-alkane-utilizable yeast, Candida tropicalis, induced gene expression in another yeast, Saccharomyces cerevisiae, when the yeasts were grown on acetate. Surprisingly, UPR-ICL displayed the same regulatory function in the bacterium Escherichia co...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00404204

    authors: Atomi H,Umemura K,Higashijima T,Kanai T,Yotsumoto Y,Teranishi Y,Ueda M,Tanaka A

    更新日期:1995-05-01 00:00:00

  • Adenine nucleotide metabolism in Azotobacter vinelandii. Two metabolic pathways of AMP degradation.

    abstract::AMP-degrading pathways in Azotobacter vinelandii cells were investigated. AMP nucleosidase (EC 3.2.2.4) was rapidly synthesized and reached a maximum at 24 h, while the activity of 5'-nucleotidase (EC 3.1.3.5) specific for AMP, which was negligible during the logarithmic phase of the growth, first appeared in 24 h-cul...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00406162

    authors: Yoshino M,Tsukada T,Murakami K,Tsushima K

    更新日期:1980-12-01 00:00:00

  • Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels.

    abstract::Previous work has shown that the mechanosensitive (MS) channel of large conductance (MscL) is essential for preventing lysis of Bacillus subtilis log phase cells upon a rapid, severe osmotic downshock. Growing cells of B. subtilis strains lacking MscL and one or more putative MS channel proteins of small conductance (...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-007-0292-z

    authors: Wahome PG,Setlow P

    更新日期:2008-01-01 00:00:00

  • Anabaena sp. PCC7120 transformed with glycine methylation genes from Aphanothece halophytica synthesized glycine betaine showing increased tolerance to salt.

    abstract::Photosynthetic, nitrogen-fixing Anabaena strains play an important role in the carbon and nitrogen cycles in tropical paddy fields although they are salt sensitive. Improvement in salt tolerance of Anabaena cells by expressing glycine betaine-synthesizing genes is an interesting subject. Due to the absence of choline ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-012-0824-z

    authors: Waditee-Sirisattha R,Singh M,Kageyama H,Sittipol D,Rai AK,Takabe T

    更新日期:2012-11-01 00:00:00

  • Taxonomic structure and function of seed-inhabiting bacterial microbiota from common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.).

    abstract::The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-018-1493-3

    authors: Gao T,Shi XY

    更新日期:2018-08-01 00:00:00

  • Morphogenesis and alpha-tubulin gene of plasmodium in Didymium megalosporum.

    abstract::The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-0971-5

    authors: Song X,Zhong H,Wang Q,Li Y

    更新日期:2014-05-01 00:00:00

  • Cibacron blue 3G-A inhibits cell separation of gram-positive bacteria.

    abstract::A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of gram-positive bacteria other than Staphylococcus. CB added to the medium of growing cultures of strains of genus Micrococcus, Streptococcus, Lactobacillus and Bacillus...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00248617

    authors: Sugai M,Akiyama T,Komatsuzawa H,Miyake Y,Suginaka H

    更新日期:1991-01-01 00:00:00

  • High-throughput sequencing-based analysis of the composition and diversity of endophytic bacterial community in seeds of upland rice.

    abstract::Upland rice is an ecotype crop resulting from the long-term domestication and evolution of rice in dry land without a water layer. Generally, the stems and leaves are thick and luxuriant, while the leaves also typically broad and light. The root system is developed with abundant root hair, and the osmotic pressure of ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-02058-9

    authors: Wang Z,Zhu Y,Jing R,Wu X,Li N,Liu H,Zhang X,Wang W,Liu Y

    更新日期:2020-09-29 00:00:00

  • Genetic diversity of fast-growing rhizobia that nodulate soybean ( Glycine max L. Merr).

    abstract::The fast-growing Rhizobium sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of Sinorhizobium fredii, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isol...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0559-y

    authors: Saldaña G,Martinez-Alcántara V,Vinardell JM,Bellogín R,Ruíz-Sainz JE,Balatti PA

    更新日期:2003-07-01 00:00:00

  • The interaction of copper ions with Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli: an X-ray absorption near-edge structure (XANES) spectroscopy study.

    abstract::The antimicrobial properties of copper ions have been known for a long time. However, the exact mechanism of action of the transition metal on microorganisms has long been unclear. X-ray absorption near-edge structure (XANES) spectroscopy at the Cu K edge allows the determination of copper speciation in Staphylococcus...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-017-1454-2

    authors: Zanzen U,Bovenkamp-Langlois L,Klysubun W,Hormes J,Prange A

    更新日期:2018-04-01 00:00:00

  • Activity of wild-type and hybrid Bacillus thuringiensis delta-endotoxins against Agrotis ipsilon.

    abstract::Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm ( Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0543-6

    authors: de Maagd RA,Weemen-Hendriks M,Molthoff JW,Naimov S

    更新日期:2003-05-01 00:00:00

  • The capacity of phototrophic sulfur bacterium Thiocapsa roseopersicina for chemosynthesis.

    abstract::Purple sulfur bacterium Thiocapsa roseopersicina strain BBS requiring vitamin B12 may grow in the dark in media containing no other organic compounds. Under such conditions the cells oxidize sulfide and thiosulfate with the use of O2 and assimilate carbon dioxide. After 10--30s assimilation of NaH14CO3 about 60% of ra...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00454854

    authors: Kondratieva EN,Zhukov VG,Ivanovsky RN,Petushkova UP,Monosov EZ

    更新日期:1976-07-01 00:00:00

  • The pathway of formation of acetate and succinate from pyruvate by Bacteroides succinogenes.

    abstract::Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00402302

    authors: Miller TL

    更新日期:1978-05-30 00:00:00

  • On the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by Peptostreptococcus anaerobius.

    abstract::Enoate reductase present in Clostridium kluyveri and Clostridium spec. La 1 could be detected in three strains of C. tyrobutyricum and ten clostridia belonging to the groups of proteolytic and saccharolytic or proteolytic species, respectively. In C. pasteurianum, C. butyricum and C. propionicum enoate reductase could...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00419482

    authors: Giesel H,Simon H

    更新日期:1983-08-01 00:00:00

  • Cloning and characterization of two novel DNases from Streptococcus pyogenes.

    abstract::The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-002-0412-8

    authors: Hasegawa T,Torii K,Hashikawa S,Iinuma Y,Ohta M

    更新日期:2002-06-01 00:00:00

  • Induction of butane consumption in Pseudomonas butanovora.

    abstract::The induction of the enzyme activities involved in butane metabolism in Pseudomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Cells grown in each of the butane metabolites readily consumed the growth substrate and downs...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030100304

    authors: Sayavedra-Soto LA,Byrd CM,Arp DJ

    更新日期:2001-07-01 00:00:00

  • Probiotic properties of Oxalobacter formigenes: an in vitro examination.

    abstract::Oxalobacter formigenes (O. formigenes) is a nonpathogenic, Gram-negative, obligate anaerobic bacterium that commonly inhabits the human gut and degrades oxalate as its major energy and carbon source. Results from a case-controlled study suggested that lack of O. formigenes colonization is a risk factor for recurrent c...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-016-1272-y

    authors: Ellis ML,Dowell AE,Li X,Knight J

    更新日期:2016-12-01 00:00:00

  • Alkaline lipase production by novel meso-tolerant psychrophilic Exiguobacterium sp. strain (AMBL-20) isolated from glacier of northeastern Pakistan.

    abstract::Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-02133-1

    authors: Yasin MT,Ali Y,Ahmad K,Ghani A,Amanat K,Basheir MM,Faheem M,Hussain S,Ahmad B,Hussain A,Bokhari SAI

    更新日期:2020-12-15 00:00:00

  • Anoxic cell rupture of Prevotella bryantii by high-pressure homogenization protects the Na+-translocating NADH:quinone oxidoreductase from oxidative damage.

    abstract::Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na+-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQR activity and protei...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-019-01805-x

    authors: Schleicher L,Fritz G,Seifert J,Steuber J

    更新日期:2020-07-01 00:00:00

  • A temperature-sensitive DNA adenine methyltransferase mutant of Salmonella typhimurium.

    abstract::A temperature-sensitive mutant of Salmonella typhimurium was isolated earlier after transposon mutagenesis with Tn10d Tet. The mutant D220 grows well at 28 degreesC but has a lower growth rate and forms filaments at 37 degreesC. Transposon-flanking fragments of mutant D220 DNA were cloned and sequenced. The transposon...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050607

    authors: Brawer R,Batista FD,Burrone OR,Sordelli DO,Cerquetti MC

    更新日期:1998-06-01 00:00:00

  • Production of antibiotic carbomycin from Streptomyces graminofaciens with high lipid content mutation.

    abstract::The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-02085-6

    authors: Arafat HH,Abu-Tahon MA,Isaac GS

    更新日期:2020-10-19 00:00:00

  • Antibacterial mechanism of soybean isoflavone on Staphylococcus aureus.

    abstract::Effects of different flavonoids on various bacterial strains have been extensively reported; however, the mechanism(s) of their action on bacterial cells remain largely elusive. In this study, the antibacterial mechanism of soybean isoflavone (SI) on Staphylococcus aureus is systematically investigated using 4'6-diami...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-010-0617-1

    authors: Wang Q,Wang H,Xie M

    更新日期:2010-11-01 00:00:00

  • Exploring the Penicillium marneffei genome.

    abstract::Penicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies o...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0533-8

    authors: Yuen KY,Pascal G,Wong SS,Glaser P,Woo PC,Kunst F,Cai JJ,Cheung EY,Médigue C,Danchin A

    更新日期:2003-05-01 00:00:00

  • Studies on the macroconidia of Microsporum canis. Characteristics of in vitro amino acid incorporating system.

    abstract::The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of 14C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00413276

    authors: Jayaram BM,Rao GR

    更新日期:1979-01-16 00:00:00