Abstract:
:AMP-degrading pathways in Azotobacter vinelandii cells were investigated. AMP nucleosidase (EC 3.2.2.4) was rapidly synthesized and reached a maximum at 24 h, while the activity of 5'-nucleotidase (EC 3.1.3.5) specific for AMP, which was negligible during the logarithmic phase of the growth, first appeared in 24 h-cultures, and reached a maximum after complete exhaustion of sucrose from the growth medium (70 h). Cell-free extracts of A. vinelandii of 48 h-cultures hydrolyzed AMP to ribose 5-phosphate and adenine in the presence of ATP, and adenine was deaminated to hypoxanthine. When ATP was excluded, AMP was dephosphorylated to adenosine, which was further metabolized to inosine, and finally to hypoxanthine. Hypoxanthine thus formed was reutilized for the salvage synthesis of IMP under the conditions where 5-phosphoribosyl 1-pyrophosphate was able to be supplied. These results suggest that the levels of ATP can determine the rate of AMP degradation by the AMP nucleosidase- and 5-'nucleotidase-pathways. The role of ATP in the AMP degradation was discussed in relation to the regulatory properties of AMP nucleosidase, inosine nucleosidase (EC. 3.2.2.2) and adenosine deaminase (EC 3.5.4.4).
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Yoshino M,Tsukada T,Murakami K,Tsushima Kdoi
10.1007/BF00406162subject
Has Abstractpub_date
1980-12-01 00:00:00pages
222-7issue
2eissn
0302-8933issn
1432-072Xjournal_volume
128pub_type
杂志文章abstract::CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28 degrees C; but at 4 degrees C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was tra...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-010-0591-7
更新日期:2010-08-01 00:00:00
abstract::During the development of fruit bodies of the basidiomycete Schizophyllum commune, the alkali-insoluble (R glucan) and alkali-soluble (S glucan) cell wall fractions are synthesized during the entire course of morphogenesis. The water soluble glucan (WSG) is not synthesized after an early stage. There is also a relativ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429623
更新日期:1977-07-26 00:00:00
abstract::The ability of soil microorganisms to solubilize phosphate is an important trait of plant growth-promoting bacteria leading to increased yields and smaller use of fertilizers. This study presents the isolation and characterization of phosphobacteria from Puna, northwestern Argentina and the ability to produce phosphat...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-011-0692-y
更新日期:2011-07-01 00:00:00
abstract::To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-008-0406-2
更新日期:2008-11-01 00:00:00
abstract::Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytopl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425171
更新日期:1989-01-01 00:00:00
abstract::Twelve Cry1 and two Cry9 delta-endotoxins from Bacillus thuringiensis were tested for their activity against black cutworm ( Agrotis ipsilon). A. ipsilon was not susceptible to many toxins, but three toxins had significant activity. Cry9Ca was the most toxic, followed by Cry1Aa and Cry1Fb. Hybrids between these three ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0543-6
更新日期:2003-05-01 00:00:00
abstract::Washed cells prepared from carbon-limited continuous cultures of Alcaligenes eutrophus synthesised poly-3-hydroxybutyrate (PHB) rapidly when supplied with glucose, DL-lactate or L-lactate. Unlike growing cultures, washed cells excreted significant amounts of pyruvate. The combined rates of PHB production (qPHB) and py...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050526
更新日期:1997-12-01 00:00:00
abstract::Purple sulfur bacterium Thiocapsa roseopersicina strain BBS requiring vitamin B12 may grow in the dark in media containing no other organic compounds. Under such conditions the cells oxidize sulfide and thiosulfate with the use of O2 and assimilate carbon dioxide. After 10--30s assimilation of NaH14CO3 about 60% of ra...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454854
更新日期:1976-07-01 00:00:00
abstract::An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050745
更新日期:1999-08-01 00:00:00
abstract::A LD-carboxypeptidase from Escherichia coli K 12 was isolated by Tris-EDTA treatment and purified to electrophoretic homogeneity by DEAE-cellulose chromatography. The enzyme has a molecular weight of approximately 12,000 as determined by sodium dodecyl sulfate-polyacrylamide electrophoresis and by Sephadex G-100 gel f...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00414732
更新日期:1986-03-01 00:00:00
abstract::Soils are rich in organics, particularly those that support the growth of plants. These organics are possible sources of sustainable energy, and a microbial fuel cell (MFC) system can potentially be used for this purpose. In this, the soil organic content expelled from plant root was possibly converted into electrical...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01947-3
更新日期:2020-10-01 00:00:00
abstract::Pyrophosphate (PPi) content of Escherichia coli is increased manyfold when the growth of the cells is limited by inhibition of the synthesis of nucleotides. The accumulated PPi is immediately degraded when inhibition is released and growth allowed to resume. Other tested nutritional deficiencies (starvation of carbon ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409846
更新日期:1983-11-01 00:00:00
abstract::Chemical mutagenesis of the nucleotide-producing strain Corynebacterium ammoniagenes ATCC 6872 with N-methyl-N-nitro-N-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. A rapid assay for the allosterically regulated Mn-ribonucleotide reductase (RRase) was developed with ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050450
更新日期:1997-05-01 00:00:00
abstract::Methanotrophs are ubiquitous in the environment and play an important role in mitigating global warming due to methane. They are also potentially interesting for industrial applications such as production of bulk chemicals or bioremediation. The first step in the oxidation of methane is the conversion to methanol by m...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030000158
更新日期:2000-05-01 00:00:00
abstract::The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the re...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0301-2
更新日期:2008-02-01 00:00:00
abstract::Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425354
更新日期:1987-07-01 00:00:00
abstract::To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000236
更新日期:2001-01-01 00:00:00
abstract::The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0695-z
更新日期:2004-09-01 00:00:00
abstract::Among proteins specifically found in most gram-positive bacteria of the phylum Firmicutes, conserved proteins of the family pfam06177-DUF988-COG4708 are of unknown function. The citrulline cluster-linked (ccl) gene of Lactobacillus plantarum codes one such protein and is adjacent to the citrulline biosynthesis operon ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0774-9
更新日期:2005-08-01 00:00:00
abstract::The effects of the Ca(2+)/H(+) exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of Phycomyces blakesleeanus were analyzed. While all of the compou...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0609-5
更新日期:2003-12-01 00:00:00
abstract::The differential gene transcription of a deep-sea bacterium Shewanella piezotolerans WP3 in response to cold shock was analyzed by RNA arbitrarily primed PCR. Ninety primer sets were used to scan two different RNA pools derived from the culture of cold shock and its control (culture at its optimal grown temperature). ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0347-1
更新日期:2008-06-01 00:00:00
abstract::The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245161
更新日期:1992-01-01 00:00:00
abstract::Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100252
更新日期:2001-03-01 00:00:00
abstract::The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2018-08-01 00:00:00
abstract::Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02003-w
更新日期:2021-01-01 00:00:00
abstract::Light-limited batch cultures of Synechococcus spp. strains PCC 6301 and WH 8103 exhibited a positive correlation between the specific growth rate and the cellular content of both RNA and DNA. The ratio of RNA to DNA increased with the growth rate in Synechococcus sp. strain WH 8103, as it does in heterotrophic bacteri...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050634
更新日期:1998-09-01 00:00:00
abstract::Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2004-10-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1181-5
更新日期:2016-04-01 00:00:00
abstract::Reciprocal qualitative and quantitative immunological experiments employing an anti-Pediococcus cerevisiae aldolase serum confirmed many of the interspecific relationships demonstrated previously among lactic acid bacteria with antisera prepared against the Streptococcus faecalis fructose diphosphate aldolase. The ext...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416976
更新日期:1976-10-11 00:00:00
abstract::A monoclonal antibody obtained by immunization of mice with heat-killed cells of Listeria monocytogenes serotype 4d showed reactivity towards a protein (P45) from L. monocytogenes with an apparent molecular mass of 45 kDa. This protein was detected in the culture supernatant and at the cell surface of L. monocytogenes...
journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00