Abstract：
:Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Expression of moeA-lacZ increased during anaerobic growth (2.5-fold over the aerobic value) and in the presence of nitrate and trimethylamine N-oxide (3.5- and 1.5-fold, respectively). The nitrate-dependent increase in moe expression required the NarL protein, while the anaerobiosis-dependent increase in moeA-lacZ expression required Arc proteins. ArcA-phosphate and not ArcA bound to the DNA upstream of moe, shifted the electrophoretic mobility of moe promoter DNA, and protected the DNA from DNase I hydrolysis. Nitrate-independent transcription of moeA-lacZ was repressed by the FNR protein, which also protected moe operator DNA from DNase I hydrolysis. These results show that ArcA-phosphate and FNR have opposite effects on the transcriptional regulation of the moe operon, and the combined action of the two redox regulators modulate the level of Mo-cofactor in the cell. Apparently, the control of synthesis of Mo-cofactor and the apomolybdoenzymes nitrate reductase and trimethylamine N-oxide reductase are coupled at the level of the moe operon.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Hasona A,Self WT,Shanmugam KTdoi
10.1007/s002030100252keywords:
subject
Has Abstractpub_date
2001-03-01 00:00:00pages
178-88issue
3eissn
0302-8933issn
1432-072Xjournal_volume
175pub_type
杂志文章abstract：:Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02003-w
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0355-5
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abstract：:TonB-dependent receptors in concert with the TonB-ExbB-ExbD protein complex are responsible for the uptake of iron and substances such as vitamin B12 in several bacterial species. In this study, Tn5 mutagenesis of the sugarcane endophytic bacterium Gluconacetobacter diazotrophicus led to the isolation of a mutant with...
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journal_title：Archives of microbiology
pub_type： 信件
doi：10.1007/s00203-019-01651-x
更新日期：2019-08-01 00:00:00
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journal_title：Archives of microbiology
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abstract：:Bioleaching is a promising process for 350 million tons Jinchuan low-grade pentlandite. But, Jinchuan pentlandite has lots of magnesium and high concentration of Mg2+ is harmful to bioleaching microorganisms. Thus, finding a way to improve the adaption of microorganisms to Mg2+ is a key for bioleaching. In the study, ...
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doi：10.1007/s00203-018-1503-5
更新日期：2018-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-015-1131-2
更新日期：2015-09-01 00:00:00
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journal_title：Archives of microbiology
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pub_type： 杂志文章
doi：10.1007/s00203-016-1329-y
更新日期：2017-05-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416976
更新日期：1976-10-11 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446549
更新日期：1976-12-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413136
更新日期：1989-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1299-0
更新日期：2017-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002039900113
更新日期：2000-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-014-0967-1
更新日期：2014-04-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0499-2
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407757
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0878-6
更新日期：2013-05-01 00:00:00
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pub_type： 杂志文章
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402127
更新日期：1984-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00403210
更新日期：1980-03-01 00:00:00
abstract：:The TTA codon, one of the six available codons for the amino acid leucine, is the rarest codon among the high GC genomes of Actinobacteria including Frankia. This codon has been implicated in various regulatory mechanisms involving secondary metabolism and morphological development. TTA-mediated gene regulation is wel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0731-8
更新日期：2012-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00690224
更新日期：1976-11-02 00:00:00
abstract：:YfkE, a protein from Bacillus subtilis, exhibits homology to the Ca(2+):Cation Antiporter (CaCA) Family. In a fluorescence-based assay of everted membrane vesicles prepared from Na(+)(Ca(2+))/H(+) antiporter-defective mutant Escherichia coli KNabc, YfkE exhibited robust Ca(2+)/H(+) antiport activity, with a K (m) for ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0494-7
更新日期：2009-08-01 00:00:00
abstract：:HaloLex is a software system for the central management, integration, curation, and web-based visualization of genomic and other -omics data for any given microorganism. The system has been employed for the manual curation of three haloarchaeal genomes, namely Halobacterium salinarum (strain R1), Natronomonas pharaoni...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0389-z
更新日期：2008-09-01 00:00:00
abstract：:The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-018-1493-3
更新日期：2018-08-01 00:00:00
abstract：:The catabolic products of arginine metabolism were observed in Aphanocapsa 6308, a unicellular cyanobacterium, by thin layer chromatography of growth media, by limiting growth conditions, and by enzymatic analysis. Of the organic, nitrogenous compounds examined, only arginine supported growth in CO2-free media. The ex...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406066
更新日期：1978-07-01 00:00:00
abstract：:DNA acquisition promotes the spread of resistance to antibiotics and virulence among bacteria. It is also linked to several natural phenomena including recombination, genome dynamics, adaptation and speciation. Horizontal DNA transfer between bacteria occurs via conjugation, transduction or competence for natural tran...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-013-0870-1
更新日期：2013-04-01 00:00:00
