Abstract：
:The effects of the Ca(2+)/H(+) exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of Phycomyces blakesleeanus were analyzed. While all of the compounds inhibited the growth of germlings in liquid medium, the Ca(2+) channel blockers were the least effective. Chitin synthesis in vivo was also sensitive to the inhibitors; here again, the calcium channel blockers were less efficient, and their effect occurred after a lag phase, in contrast to the electroneutral ionophores whose effects were immediate. The ionophores rapidly inhibited protein secretion, and reduced the number of secretory vesicles and chitosomes in the hyphal apex of P. blakesleeanus. The results suggest that not only tip-to-base calcium gradients but also transmembrane ionic gradients and membrane potential have a role in the apical growth of P. blakesleeanus. They are probably involved in the formation, migration, and/or fusion with the plasmalemma of secretory vesicles and chitosomes.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Ruiz-Herrera J,Martínez-Cadena G,Loarca F,Salcedo-Hernández Rdoi
10.1007/s00203-003-0609-5keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
427-33issue
6eissn
0302-8933issn
1432-072Xjournal_volume
180pub_type
杂志文章abstract：:A 2.3-L bioreactor was specially adapted to grow hyperthermophilic microorganisms under controlled conditions of temperature, pH, redox potential and dissolved O(2). Using this bioreactor regulated at 80°C and pH 7.0, we demonstrated that Thermotoga maritima recovered its growth despite being exposed to oxygen for a s...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0687-8
更新日期：2011-06-01 00:00:00
abstract：:The gliding bacterium Herpetosiphon giganteus Hp a2 was shown to contain gamma-carotene, one monoglucosyloxy and two new diglucosyloxy carotenoids with a x-O-acyldiglucosyloxy carotenoid as main component. Flexirubin-like pigments could not be detected in this organism. As in the Myxobacterales and Cytophagales (Klein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413098
更新日期：1977-04-01 00:00:00
abstract：:Polysomes were isolated both from growing gametophytes of Allomyces arbuscula and from gametangia prepared from mycelia at different periods during gametogenesis. Analysis of polysomes by sucrose gradients showed that ribosomes present in the gametangia monosome pool were shifted into polysomes. This shift was found t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413089
更新日期：1977-04-01 00:00:00
abstract：:With primers designed for the conserved region of the gamma-butyrolactone autoregulator receptor proteins from Streptomyces species, PCR using the Streptomyces clavuligerus genome DNA as a template gave a clear band of 100 bp, the sequence of which revealed high similarity to the expected region of a receptor gene. By...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0697-x
更新日期：2004-09-01 00:00:00
abstract：:A complex polydispersity became apparent when the poly(glycerophosphate) lipoteichoic acid of Enterococcus faecalis was chromatographed on DEAE-Sephadex. The chain length varied between 13 and 33 glycerophosphate residues per lipid anchor. In parallel, the extent of chain glycosylation increased from 0.2 to 0.4 digluc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249103
更新日期：1992-01-01 00:00:00
abstract：:Histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. An antigenic surface layer has been visualized in the cell wall of immunostained organisms. This layer was not seen in samples prepared by standard electro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00693397
更新日期：1985-08-01 00:00:00
abstract：:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428588
更新日期：1977-05-13 00:00:00
abstract：:The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446920
更新日期：1989-01-01 00:00:00
abstract：:Two styrene monooxygenase types, StyA/StyB and StyA1/StyA2B, have been described each consisting of an epoxidase and a reductase. A gene fusion which led to the chimeric reductase StyA2B and the occurrence in different phyla are major differences. Identification of SMOA/SMOB-ADP1 of Acinetobacter baylyi ADP1 may enlig...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1022-y
更新日期：2014-12-01 00:00:00
abstract：:Many pathogenic bacteria express filamentous appendages, termed pili, on their surface. These organelles function in several important bacterial processes, including mediating bacterial interaction with, and colonization of the host, signalling events, locomotion, DNA uptake, electric conductance, and biofilm formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-015-1117-0
更新日期：2015-08-01 00:00:00
abstract：:Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410891
更新日期：1984-08-01 00:00:00
abstract：:Pure cultures of an aerobically grown Flavobacterium sp. were shown by hydride generation-cold trap-atomic absorption spectrometry to biomethylate inorganic antimony (III) supplied as potassium antimony tartrate. Growth inhibition of the Flavobacterium sp. by antimony (III) over the range 0-30 mg Sb l(-1) was assessed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0456-9
更新日期：2002-10-01 00:00:00
abstract：:The symbiotic bacteria Bradyrhizobium japonicum and Sinorhizobium meliloti, and the purple photosynthetic bacteria Rhodobacter capsulatus, Rhodovulum sulfidophilum, Roseobacter denitrificans and Rhodobacter sphaeroides possess homologous two-component regulatory systems, namely RegSR, ActSR, RegBA and PrrBA. The respe...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000207
更新日期：2000-11-01 00:00:00
abstract：:An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050745
更新日期：1999-08-01 00:00:00
abstract：:We report on the genome-wide response, based on DNA microarrays, of the cyanobacterium Synechocystis sp. PCC 6803 wild type and DeltasigB to a 15 min heat shock. Approximately 9% of the genes in wild type and DeltasigB were significantly regulated (P < 0.001) following this treatment, with chaperones induced the most....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0138-0
更新日期：2006-10-01 00:00:00
abstract：:Glucose and other sugars, such as galactose or maltose, are able to cause carbon catabolite repression in Saccharomyces cerevisiae. Although glycolytic intermediates have been suggested as signal for repression, no evidence for such a control mechanism is available. The establishment of a correlation between levels of...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0593-9
更新日期：2003-10-01 00:00:00
abstract：:Cyanothece sp. strain ATCC 51142 is an aerobic, unicellular, diazotrophic cyanobacterium that temporally separates O2-sensitive N2 fixation from oxygenic photosynthesis. The energy and reducing power needed for N2 fixation appears to be generated by an active respiratory apparatus that utilizes the contents of large i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Short-chain aliphatic epoxides and ketones are two classes of toxic organic compounds formed biogenically and anthropogenically. In spite of their toxicity, these compounds are utilized as primary carbon and energy sources or are generated as intermediate metabolites in the metabolism of other compounds (e.g., alkenes...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050558
更新日期：1998-03-01 00:00:00
abstract：:To clarify the deactivation mechanism of pyruvate formate-lyase (PFL) and its role in the regulation of fermentation in Streptococcus bovis, the molecular properties and genetic expression of multifunctional alcohol dehydrogenase (ADHE) were investigated. S. bovis was found to have ADHE, which was deduced to consist o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0638-0
更新日期：2004-02-01 00:00:00
abstract：:The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01833-y
更新日期：2020-07-01 00:00:00
abstract：:In the fermentation of L-aspartate by a free-living Campylobacter spec., the products formed were acetate, succinate, carbon dioxide and ammonia. The oxidative part of the fermentation pathway yielded acetate, succinate, carbon dioxide and ammonia, and the reductive part gave rise to the formation of succinate and amm...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00689359
更新日期：1978-04-27 00:00:00
abstract：:Two proteases, designated I and II, have been isolated from sporulating cells of Bacillus subtilis. They were partially purified by ammonium sulfate fractionation, Sephadex chromatography and affinity columns. Protease I was found to be similar to an already characterized B. subtilis protease. Protease II is trypsin-l...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425256
更新日期：1981-05-01 00:00:00
abstract：:Purified flagellar filaments isolated from six methanogens were composed of multiple flagellins. Two flagellins were present in Methanococcus deltae (Mr = 34,000 and 32,000), Methanoculleus marisnigri (Mr = 31,000 and 25,500) and Methanococcus jannaschii (Mr = 31,000 and 27,500), three in Methanothermus fervidus (Mr =...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00276766
更新日期：1992-01-01 00:00:00
abstract：:The time dependent assembly of the photosynthetic apparatus was studied in Rhodospirillum rubrum after transfer of cells growing aerobically in the dark to low aeration. While bacteriochlorophyll (Bchl) cellular levels increase continuously levels of soluble cytochrome C2 do not change significantly. Absorption spectr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454853
更新日期：1976-07-01 00:00:00
abstract：:The acidic amino acids (Asp, Glu) and their amides (Asn, Gln) support rapid growth of a variety of Pseudomonas strains when provided as the sole source of carbon and nitrogen. All key enzymes of glutamate metabolism were detected in P. fluorescence, with glutaminase and asparaginase showing the highest specific activi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0511-6
更新日期：2003-03-01 00:00:00
abstract：:The ability of the imperfect soil fungus Paecilomyces lilacinus to transform the environmental pollutant dibenzofuran was investigated. Transformation of dibenzofuran and related derivatives lead to 14 products, which were identified by UV spectroscopy, mass spectrometry, and proton nuclear magnetic resonance spectros...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0695-z
更新日期：2004-09-01 00:00:00
abstract：:We have investigated the endogenous phosphorylation patterns of phosphorylated proteins of Xanthomonas campestris pv. oryzae induced by its bacteriophages. For bacteriophage Xp12-infected cells, at least three phosphoproteins with apparent molecular weights of 28, 28.5 and 45 kDa were detected by in vitro labeling wit...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00303581
更新日期：1994-01-01 00:00:00
abstract：:Aerobic anoxygenic phototrophs (AAPs) are prokaryotic microorganisms capable of harvesting light using bacteriochlorophyll-based reaction centres. Marine AAP communities are generally dominated by species belonging to the Roseobacter clade. For this reason, we used marine Roseobacter-related strain COL2P as a model or...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0529-0
更新日期：2010-01-01 00:00:00
abstract：:Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 ha...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01794-x
更新日期：2020-05-01 00:00:00
abstract：:The mechanism of lysis of Eubacterium alactolyticum cell walls by Streptomyces albus G enzyme was studied. The analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of S. albus G enzyme was mainly du...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406563
更新日期：1989-01-01 00:00:00
