Abstract：
:The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of 14C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic acid. Incorporation is slightly enhanced by yeast transfer ribonucleic acid and pyruvate kinase. The system is highly sensitive to ribonuclease, puromycin and miconazole (an antifungal agent), moderately sensitive to sodium fluoride and much less sensitive to phenethylalcohol, cycloheximide, chloramphenicol and deoxyribonuclease. Cell-free extract from ungerminated conidia has less capacity to synthesize the protein and during germination a marked increase in the protein synthetic activity is observed. The results from experiments wherein ribosomes and S-100 fraction from germinated and ungerminated spores are unterchanged, revealed that the defect in the extract from the ungerminated spore is in the ribosomes.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Jayaram BM,Rao GRdoi
10.1007/BF00413276subject
Has Abstractpub_date
1979-01-16 00:00:00pages
67-72issue
1eissn
0302-8933issn
1432-072Xjournal_volume
120pub_type
杂志文章abstract：:Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titrat...
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
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doi：10.1007/BF00447134
更新日期：1975-10-27 00:00:00
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更新日期：1988-01-01 00:00:00
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更新日期：1980-06-01 00:00:00
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更新日期：2015-05-01 00:00:00
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更新日期：2017-04-01 00:00:00
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更新日期：1996-10-17 00:00:00
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doi：10.1007/BF00446551
更新日期：1976-12-01 00:00:00
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更新日期：1999-05-01 00:00:00
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更新日期：2020-10-01 00:00:00
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更新日期：2011-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00415280
更新日期：1987-03-01 00:00:00
abstract：:Bacterial pathogens are a major threat to both humans and animals worldwide. It is crucial to understand the mechanisms of various disease processes at the molecular level. Shewanella species are widespread in the environment and some are considered as emerging opportunistic human and marine mammal pathogens. In this ...
journal_title：Archives of microbiology
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更新日期：2020-10-10 00:00:00
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journal_title：Archives of microbiology
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更新日期：1977-03-01 00:00:00
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更新日期：2020-09-24 00:00:00
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更新日期：2020-10-15 00:00:00
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更新日期：1977-03-01 00:00:00
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更新日期：2020-12-01 00:00:00
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doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407929
更新日期：1978-10-04 00:00:00
abstract：:In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity...
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pub_type： 杂志文章
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更新日期：1996-12-01 00:00:00
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