A temperature-sensitive DNA adenine methyltransferase mutant of Salmonella typhimurium.

Abstract:

:A temperature-sensitive mutant of Salmonella typhimurium was isolated earlier after transposon mutagenesis with Tn10d Tet. The mutant D220 grows well at 28 degreesC but has a lower growth rate and forms filaments at 37 degreesC. Transposon-flanking fragments of mutant D220 DNA were cloned and sequenced. The transposon was inserted in the dam gene between positions 803 and 804 (assigned allele number: dam-231 : : Tn10d Tet) and resulted in a predicted ten-amino-acid-shorter Dam protein. The insertion created a stop codon that led to a truncated Dam protein with a temperature-sensitive phenotype. The insertion dam-231 : : Tn10d Tet resulted in a dam "leaky" phenotype since methylated and unmethylated adenines in GATC sequences were present. In addition, the dam-231 : : Tn10d Tet insertion rendered dam mutants temperature-sensitive for growth depending upon the genetic background of the S. typhimurium strain. The wild-type dam gene of S. typhimurium exhibited 82% identity with the Escherichia coli dam gene.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Brawer R,Batista FD,Burrone OR,Sordelli DO,Cerquetti MC

doi

10.1007/s002030050607

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

530-3

issue

6

eissn

0302-8933

issn

1432-072X

journal_volume

169

pub_type

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