Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants (PPR) virus in cell culture.

Abstract:

:Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titration in Vero cells and the viral presence was confirmed by real-time RT-PCR. The virus was first detected in both the supernatants and cell pellets at 12 hpi. The virus titre reached its plateau at 72 hpi. Maximum virus titre of CAV was 6.2 log10 TCID50/ml and that of CFV was 5.2 log10 TCID50/ml at 72 hpi. After that, the titer gradually declined, but maintained at 4.5 log10 TCID50/ml in case of CAV and 4.2 log10 TCID50/ml in case of CFV at 96 hpi. It was concluded that the optimum time point for harvesting Vero cell culture is 72 hpi.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Siddiqui MSI,Islam MR,Chowdhury EH

doi

10.1007/s00203-020-02154-w

subject

Has Abstract

pub_date

2021-01-05 00:00:00

eissn

0302-8933

issn

1432-072X

pii

10.1007/s00203-020-02154-w

pub_type

杂志文章
  • Genetic control of lysine permeases in Saccharomycopsis lipolytica.

    abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00411361

    authors: Beckerich JM,Lambert M,Heslot H

    更新日期:1979-08-06 00:00:00

  • Desulfitobacterium sp. strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols.

    abstract::A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050308

    authors: Gerritse J,Renard V,Pedro Gomes TM,Lawson PA,Collins MD,Gottschal JC

    更新日期:1996-02-01 00:00:00

  • Isolation and identification of a bacteriocin with antibacterial and antibiofilm activity from Citrobacter freundii.

    abstract::Multi- and pan-antibiotic-resistant bacteria area major health challenge in hospital settings. Furthermore,when susceptible bacteria establish surface-attached biofilm populations, they become recalcitrant to antimicrobial therapy. Therefore, there is a need for novel antimicrobials that are effective against multi-dr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-012-0793-2

    authors: Shanks RM,Dashiff A,Alster JS,Kadouri DE

    更新日期:2012-07-01 00:00:00

  • Characterization of cost with respect to nutritional upshift in the media composition along with sublethal doses of transcriptional and translational inhibitor.

    abstract::Expression of unnecessary proteins is known to reduce the growth rate of Escherichia coli. This reduction in growth rate, due to the diversion of cellular resources from making essential proteins, is called cost. Cellular resources depend upon the macromolecular content of the cell. Cost phenomenon lies in the process...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-0967-1

    authors: Malakar P

    更新日期:2014-04-01 00:00:00

  • Physiological roles of mycothiol in detoxification and tolerance to multiple poisonous chemicals in Corynebacterium glutamicum.

    abstract::Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-013-0889-3

    authors: Liu YB,Long MX,Yin YJ,Si MR,Zhang L,Lu ZQ,Wang Y,Shen XH

    更新日期:2013-06-01 00:00:00

  • Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis.

    abstract::We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca(2+)-...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-009-0498-3

    authors: Hermansyah,Sugiyama M,Kaneko Y,Harashima S

    更新日期:2009-09-01 00:00:00

  • Coniochaeta fungus benefits from its intracellular bacteria to form biofilm and defend against other fungi.

    abstract::During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-02122-4

    authors: Heydari S,Siavoshi F,Sarrafnejad A,Malekzadeh R

    更新日期:2021-01-02 00:00:00

  • The occurrence of teichoic acids in streptomycetes.

    abstract::The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00421893

    authors: Naumova IB,Kuznetsov VD,Kudrina KS,Bezzubenkova AP

    更新日期:1980-05-01 00:00:00

  • Sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.

    abstract::Recent studies indicated that bioluminescence of the marine bacterium Vibrio harveyi may both stimulate DNA repair and contribute to detoxification of deleterious oxygen derivatives. Therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolu...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-005-0764-y

    authors: Lyzeń R,Wegrzyn G

    更新日期:2005-03-01 00:00:00

  • Influence of long-term organic and conventional fertilization on bacterial communities involved in bioelectricity production from paddy field-microbial fuel cells.

    abstract::Soils are rich in organics, particularly those that support the growth of plants. These organics are possible sources of sustainable energy, and a microbial fuel cell (MFC) system can potentially be used for this purpose. In this, the soil organic content expelled from plant root was possibly converted into electrical...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-01947-3

    authors: Kamaraj Y,Punamalai G,Kandasamy S,Kasinathan K

    更新日期:2020-10-01 00:00:00

  • Carboxin resistance in Paracoccus denitrificans conferred by a mutation in the membrane-anchor domain of succinate:quinone reductase.

    abstract::Succinate:quinone reductase is a membrane-bound enzyme of the citric acid cycle and the respiratory chain. Carboxin is a potent inhibitor of the enzyme of certain organisms. The bacterium Paracoccus denitrificans was found to be sensitive to carboxin in vivo, and mutants that grow in the presence of 3'-methyl carboxin...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050611

    authors: Matsson M,Ackrell BA,Cochran B,Hederstedt L

    更新日期:1998-07-01 00:00:00

  • Genetic diversity of Microcystis cyanophages in two different freshwater environments.

    abstract::Bacteriophages rapidly diversify their genes through co-evolution with their hosts. We hypothesize that gene diversification of phages leads to locality in phages genome. To test this hypothesis, we investigated the genetic diversity and composition of Microcystis cyanophages using 104 sequences of Ma-LMM01-type cyano...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-0980-4

    authors: Nakamura G,Kimura S,Sako Y,Yoshida T

    更新日期:2014-06-01 00:00:00

  • Characterization of Aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge.

    abstract::A gram-negative bacterium was isolated from activated sewage sludge with thiophene-2-carboxylate as the sole source of carbon and with nitrate as an electron acceptor. The isolate, strain NKK, was a motile, oxidase- and catalase-positive, rod-like bacterium with a G+C content of 61.7 mol%. Besides nitrate, oxygen coul...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050575

    authors: Bambauer A,Rainey FA,Stackebrandt E,Winter J

    更新日期:1998-04-01 00:00:00

  • Characterization and structural properties of the major biliproteins of Anabaena sp.

    abstract::Studies are presented of the biliproteins of Anabaena sp. This filamentous cyanobacterium contains three major biliproteins. Whereas two of these, C-phycocyanin and allophycocyanin, are common to all cyanobacteria, the third, phycoerythrocyanin (gammamax approximately 568 nm) has hitherto not been described and its di...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00416970

    authors: Bryant DA,Glazer AN,Eiserling FA

    更新日期:1976-10-11 00:00:00

  • Role of respiration and glutathione in cadmium-induced oxidative stress in Escherichia coli K-12.

    abstract::Cadmium is a widespread pollutant that has been associated with oxidative stress, but the mechanism behind this effect in prokaryotes is still unclear. In this work, we exposed two glutathione deficient mutants (DeltagshA and DeltagshB) and one respiration deficient mutant (DeltaubiE) to a sublethal concentration of c...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-007-0316-8

    authors: Pacheco CC,Passos JF,Castro AR,Moradas-Ferreira P,De Marco P

    更新日期:2008-03-01 00:00:00

  • Accumulation of pyrophosphate in Escherichia coli. Relationship to growth and nucleotide synthesis.

    abstract::Pyrophosphate (PPi) content of Escherichia coli is increased manyfold when the growth of the cells is limited by inhibition of the synthesis of nucleotides. The accumulated PPi is immediately degraded when inhibition is released and growth allowed to resume. Other tested nutritional deficiencies (starvation of carbon ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00409846

    authors: Kukko E,Saarento H

    更新日期:1983-11-01 00:00:00

  • Use of an acidophilic yeast strain to enable the growth of leaching bacteria on solid media.

    abstract::In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including o...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-1051-6

    authors: Ngom B,Liang Y,Liu Y,Yin H,Liu X

    更新日期:2015-03-01 00:00:00

  • Virulent strains of Salmonella enteritidis disrupt the epithelial barrier of Caco-2 and HEp-2 cells.

    abstract::To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030000236

    authors: Solano C,Sesma B,Alvarez M,Urdaneta E,Garcia-Ros D,Calvo A,Gamazo C

    更新日期:2001-01-01 00:00:00

  • A mutated hygromycin resistance gene is functional in the n-alkane-assimilating yeast Candida tropicalis.

    abstract::Development of a transformation system in the n-alkane-assimilating diploid yeast Candida tropicalis requires an antibiotic resistance gene in order to establish a selectable marker. The resistance gene for hygromycin B has often been used as a selectable marker in yeast transformation. However, C. tropicalis harborin...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002039900125

    authors: Hara A,Ueda M,Misawa S,Matsui T,Furuhashi K,Tanaka A

    更新日期:2000-03-01 00:00:00

  • Congo Red affects the growth, morphology and activity of glucosamine-6-phosphate synthase in the human pathogenic fungus Sporothrix schenckii.

    abstract::Sporothrix schenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphog...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-018-1576-1

    authors: Sánchez-López JF,González-Ibarra J,Macías-Segoviano JI,Cuéllar-Cruz M,Álvarez-Vargas A,Cano-Canchola C,López-Romero E

    更新日期:2019-01-01 00:00:00

  • The complete mitochondrial genome of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae: gene order and trn gene clusters reveal a common evolutionary course for all Sordariomycetes, while intergenic regions show variation.

    abstract::The mitochondrial genome (mtDNA) of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae, with a total size of 24,673 bp, was one of the smallest known mtDNAs of Pezizomycotina. It contained the 14 typical genes coding for proteins related to oxidative phosphorylation, the two rRNA genes, a single intron...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-006-0104-x

    authors: Ghikas DV,Kouvelis VN,Typas MA

    更新日期:2006-06-01 00:00:00

  • Deletion of two downstream genes alters expression of the hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough.

    abstract::The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050458

    authors: Keon RG,Fu R,Voordouw G

    更新日期:1997-06-01 00:00:00

  • A gas vesiculate planktonic strain of the purple non-sulfur bacterium Rhodoferax antarcticus isolated from Lake Fryxell, Dry Valleys, Antarctica.

    abstract::A moderately psychrophilic purple non-sulfur bacterium, Rhodoferax antarcticus strain Fryx1, is described. Strain Fryx1 was isolated from the water column under the ice of the permanently frozen Lake Fryxell, Antarctica. Cells of Fryx1 are long thin rods and contain gas vesicles, the first report of such structures in...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-004-0719-8

    authors: Jung DO,Achenbach LA,Karr EA,Takaichi S,Madigan MT

    更新日期:2004-10-01 00:00:00

  • Microfilament bundles in antheridial nuclei of Achlya ambisexualis E 87.

    abstract::This is the first report of intranuclear microfilaments within gametangial nuclei of oömycetous fungi. Longitudinal sections of four to six microfilaments were frequently observed in meiotic antheridial nuclei of Achlya ambisexualis. Each microfilament measured approximately 7--10 nm in diameter. Spindle tubules (25 n...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00427876

    authors: Ellzey J,Huizar E,Yanez D

    更新日期:1976-02-01 00:00:00

  • Fatty acid, polar lipid and wall amino acid composition of Gardnerella vaginalis.

    abstract::Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00425410

    authors: O'Donnell AG,Minnikin DE,Goodfellow M,Piot P

    更新日期:1984-05-01 00:00:00

  • Status (on/off) of oipA gene: their associations with gastritis and gastric cancer and geographic origins.

    abstract::Virulence factors of H. pylori, such as outer inflammatory protein A (oipA), are closely involved in the development of gastric diseases such as chronic gastritis and gastric cancer. The functional status of oipA is regulated by a repair mechanism based on CT dinucleotide repeats that influence the reading frame, thus...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-018-1580-5

    authors: Sallas ML,Dos Santos MP,Orcini WA,David ÉB,Peruquetti RL,Payão SLM,Rasmussen LT

    更新日期:2019-01-01 00:00:00

  • Bacterial NAD(P)-independent quinate dehydrogenase is a quinoprotein.

    abstract::Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00409714

    authors: van Kleef MA,Duine JA

    更新日期:1988-05-01 00:00:00

  • Transcriptional regulation of the moe (molybdate metabolism) operon of Escherichia coli.

    abstract::Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030100252

    authors: Hasona A,Self WT,Shanmugam KT

    更新日期:2001-03-01 00:00:00

  • Xylose and some non-sugar carbon sources cause catabolite repression in Saccharomyces cerevisiae.

    abstract::Glucose and other sugars, such as galactose or maltose, are able to cause carbon catabolite repression in Saccharomyces cerevisiae. Although glycolytic intermediates have been suggested as signal for repression, no evidence for such a control mechanism is available. The establishment of a correlation between levels of...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-003-0593-9

    authors: Belinchón MM,Gancedo JM

    更新日期:2003-10-01 00:00:00

  • Directed modification of a ruminal cellulase gene (CMC-1) from a metagenomic library isolated from Yunnan gayal (Bos frontalis).

    abstract::Gayal (Bos frontalis) of the Yunnan region is well adapted to harsh environmental conditions. Its diet consists predominantly of bamboo, reeds, and woody plants, suggesting that the rumen of this species contains many fiber-degrading bacteria and cellulases. The aim of this study was to identify and modify specific ce...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-01812-3

    authors: Wu D,Wang S,Vinitchaikul P,Zhu Y,Zhou X,Gu Z,Leng J,Gou X,Deng M,Sun L,Mao H,Yang S

    更新日期:2020-07-01 00:00:00