Abstract:
:Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titration in Vero cells and the viral presence was confirmed by real-time RT-PCR. The virus was first detected in both the supernatants and cell pellets at 12 hpi. The virus titre reached its plateau at 72 hpi. Maximum virus titre of CAV was 6.2 log10 TCID50/ml and that of CFV was 5.2 log10 TCID50/ml at 72 hpi. After that, the titer gradually declined, but maintained at 4.5 log10 TCID50/ml in case of CAV and 4.2 log10 TCID50/ml in case of CFV at 96 hpi. It was concluded that the optimum time point for harvesting Vero cell culture is 72 hpi.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Siddiqui MSI,Islam MR,Chowdhury EHdoi
10.1007/s00203-020-02154-wsubject
Has Abstractpub_date
2021-01-05 00:00:00eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-020-02154-wpub_type
杂志文章abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00411361
更新日期:1979-08-06 00:00:00
abstract::A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050308
更新日期:1996-02-01 00:00:00
abstract::Multi- and pan-antibiotic-resistant bacteria area major health challenge in hospital settings. Furthermore,when susceptible bacteria establish surface-attached biofilm populations, they become recalcitrant to antimicrobial therapy. Therefore, there is a need for novel antimicrobials that are effective against multi-dr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0793-2
更新日期:2012-07-01 00:00:00
abstract::Expression of unnecessary proteins is known to reduce the growth rate of Escherichia coli. This reduction in growth rate, due to the diversion of cellular resources from making essential proteins, is called cost. Cellular resources depend upon the macromolecular content of the cell. Cost phenomenon lies in the process...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0967-1
更新日期:2014-04-01 00:00:00
abstract::Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-013-0889-3
更新日期:2013-06-01 00:00:00
abstract::We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca(2+)-...
journal_title:Archives of microbiology
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doi:10.1007/s00203-009-0498-3
更新日期:2009-09-01 00:00:00
abstract::During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
journal_title:Archives of microbiology
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00421893
更新日期:1980-05-01 00:00:00
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journal_title:Archives of microbiology
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doi:10.1007/s00203-005-0764-y
更新日期:2005-03-01 00:00:00
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journal_title:Archives of microbiology
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doi:10.1007/s00203-020-01947-3
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050611
更新日期:1998-07-01 00:00:00
abstract::Bacteriophages rapidly diversify their genes through co-evolution with their hosts. We hypothesize that gene diversification of phages leads to locality in phages genome. To test this hypothesis, we investigated the genetic diversity and composition of Microcystis cyanophages using 104 sequences of Ma-LMM01-type cyano...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0980-4
更新日期:2014-06-01 00:00:00
abstract::A gram-negative bacterium was isolated from activated sewage sludge with thiophene-2-carboxylate as the sole source of carbon and with nitrate as an electron acceptor. The isolate, strain NKK, was a motile, oxidase- and catalase-positive, rod-like bacterium with a G+C content of 61.7 mol%. Besides nitrate, oxygen coul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050575
更新日期:1998-04-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416970
更新日期:1976-10-11 00:00:00
abstract::Cadmium is a widespread pollutant that has been associated with oxidative stress, but the mechanism behind this effect in prokaryotes is still unclear. In this work, we exposed two glutathione deficient mutants (DeltagshA and DeltagshB) and one respiration deficient mutant (DeltaubiE) to a sublethal concentration of c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0316-8
更新日期:2008-03-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409846
更新日期:1983-11-01 00:00:00
abstract::In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including o...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1051-6
更新日期:2015-03-01 00:00:00
abstract::To confirm the existence in nature of Salmonella enteritidis strains of different degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequences of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strains in a chicken inf...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000236
更新日期:2001-01-01 00:00:00
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
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journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0104-x
更新日期:2006-06-01 00:00:00
abstract::The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050458
更新日期:1997-06-01 00:00:00
abstract::A moderately psychrophilic purple non-sulfur bacterium, Rhodoferax antarcticus strain Fryx1, is described. Strain Fryx1 was isolated from the water column under the ice of the permanently frozen Lake Fryxell, Antarctica. Cells of Fryx1 are long thin rods and contain gas vesicles, the first report of such structures in...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0719-8
更新日期:2004-10-01 00:00:00
abstract::This is the first report of intranuclear microfilaments within gametangial nuclei of oömycetous fungi. Longitudinal sections of four to six microfilaments were frequently observed in meiotic antheridial nuclei of Achlya ambisexualis. Each microfilament measured approximately 7--10 nm in diameter. Spindle tubules (25 n...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00427876
更新日期:1976-02-01 00:00:00
abstract::Representative strains of Gardnerella vaginalis were degraded using both an alkaline and an acid methanolysis and the fatty acid methyl esters released examined by thin-layer and gas chromatography. The profiles obtained were both qualitatively and quantitatively similar and were comprised of straight chain saturated ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425410
更新日期:1984-05-01 00:00:00
abstract::Virulence factors of H. pylori, such as outer inflammatory protein A (oipA), are closely involved in the development of gastric diseases such as chronic gastritis and gastric cancer. The functional status of oipA is regulated by a repair mechanism based on CT dinucleotide repeats that influence the reading frame, thus...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-018-1580-5
更新日期:2019-01-01 00:00:00
abstract::Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409714
更新日期:1988-05-01 00:00:00
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journal_title:Archives of microbiology
pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
abstract::Glucose and other sugars, such as galactose or maltose, are able to cause carbon catabolite repression in Saccharomyces cerevisiae. Although glycolytic intermediates have been suggested as signal for repression, no evidence for such a control mechanism is available. The establishment of a correlation between levels of...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0593-9
更新日期:2003-10-01 00:00:00
abstract::Gayal (Bos frontalis) of the Yunnan region is well adapted to harsh environmental conditions. Its diet consists predominantly of bamboo, reeds, and woody plants, suggesting that the rumen of this species contains many fiber-degrading bacteria and cellulases. The aim of this study was to identify and modify specific ce...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01812-3
更新日期:2020-07-01 00:00:00