Ammonia assimilation in the fission yeast Schizosaccharomyces pombe 972.

Abstract:

:Glutamine synthetase (GS) activity of Schizosaccharomyces pombe 972 was high in ammonia-limited cultures, low in phosphate- and sulphate-limited cultures and not detected in glucose-limited cultures. When ammonia was 'pulsed' into an ammonia-limited culture then GS activity decreased at a rate faster than that calculated if enzyme synthesis ceased and enzyme was diluted out by growth. Enzyme activity increased in ammonia-starved, phosphate-limited cultures and in the ammonia 'pulse' system when the added ammonia had been utilised. These increases in enzyme activity were prevented by the presence of 100 mug/ml cycloheximide. GS activity was inversely related to the intracellular concentration of glutamate.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

van Andel JG,Brown CM

doi

10.1007/BF00549365

keywords:

subject

Has Abstract

pub_date

1977-01-11 00:00:00

pages

265-70

issue

3

eissn

0302-8933

issn

1432-072X

journal_volume

111

pub_type

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