Abstract:
:Recently it was found that the specific activity of H2-forming methylenetetrahydromethanopterin dehydrogenase (Hmd) in Methanothermobacter marburgensis (formerly Methanobacterium thermoautotrophicum strain Marburg) increased six-fold when the hydrogenotrophic archaeon was grown in chemostat culture under nickel-limited conditions. We report here that the increase is due, at least in part, to increased expression of the hmd gene. This was demonstrated by Northern and Western blot analysis. These techniques were also used to show that hmd expression in growing M. marburgensis is not under the control of the H2 concentration. Studies with monoclonal antibodies on the effect of growth conditions on the expression of hmdII and hmdIII, which have been proposed to encode Hmd isoenzymes, were also carried out. The results indicate that the expression of these two genes is regulated by H2 rather than by nickel, and that HmdIII and HmdIII most probably do not exhibit Hmd activity.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Afting C,Kremmer E,Brucker C,Hochheimer A,Thauer RKdoi
10.1007/s002030000197keywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
225-32issue
4eissn
0302-8933issn
1432-072Xjournal_volume
174pub_type
杂志文章abstract::Under starvation conditions, rhodopsin-containing halobacteria show a light-accelerated death under aerobic conditions. This is attributed to photooxidative processes. Under anaerobic conditions, halobacteria die rapidly in the dark, and light prevents death. Since it has been shown by others that lightdriven ATP synt...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00425136
更新日期:1976-08-01 00:00:00
abstract::Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100252
更新日期:2001-03-01 00:00:00
abstract::The ADPglucose pyrophosphorylases from Rhodopseudomonas sphaeroides and Rhodopseudomonas gelatinosa are activated by fructose-6-phosphate, pyruvate and fructose-1,6 biophosphate-P2. The effects of the activators are to increase significantly the Vmax of ADPglucose synthesis and to lower the S0.5 values (concentration ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00421887
更新日期:1980-05-01 00:00:00
abstract::Ostracods collected from shallow coral reefs in the Bahamas were found to exhibit blue light-stimulated orange fluorescence at night. Fluorescent spectra revealed the presence of orange fluorescence with a maximum emission at ~595 nm on the carapace of these ostracods, while scanning electron microscopy revealed a mor...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-013-0911-9
更新日期:2013-09-01 00:00:00
abstract::The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02085-6
更新日期:2020-10-19 00:00:00
abstract::Coenzyme F420 is a 8-hydroxy-5-deazaflavin present in methanogenic bacteria. We have investigated whether the pyrimidine ring of the deazaflavin originates from guanine as in flavin biosynthesis, in which the pyrimidine ring of guanine is conserved. For this purpose the incorporation of [2-14C]guanine and of [8-14C]gu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00410735
更新日期:1984-04-01 00:00:00
abstract::Bacteriochlorophyll (BChl) c in whole cells of Chlorobium tepidum grown at 46 degrees C changed into bacteriopheophytin (BPhe) c within 10 days after reaching full growth. When a small amount of C. tepidum cells in which BChl c had been completely pheophytinized were transferred to a new culture medium, normal growth ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050737
更新日期:1999-07-01 00:00:00
abstract::After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492022
更新日期:1977-06-20 00:00:00
abstract::Video-enhanced light microscopy of the apical and subapical regions of growing hyphae of several fungal species revealed the existence of momentary synchronized motions of subcellular organelles. First discovered in a temperature-sensitive morphological mutant (ramosa-1) of Aspergillus niger, these seemingly spontaneo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0771-z
更新日期:2005-05-01 00:00:00
abstract::A moderately psychrophilic purple non-sulfur bacterium, Rhodoferax antarcticus strain Fryx1, is described. Strain Fryx1 was isolated from the water column under the ice of the permanently frozen Lake Fryxell, Antarctica. Cells of Fryx1 are long thin rods and contain gas vesicles, the first report of such structures in...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0719-8
更新日期:2004-10-01 00:00:00
abstract::The development of peritrichous flagella and, consequently, swarming of Vibrio alginolyticus depend on a complex relationship between temperature, salt concentrations and pH. At temperatures above 28 degrees C V. alginolyticus did not develop peritrichous flagella unless certain minimal concentrations of NaCl are pres...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447338
更新日期:1975-08-28 00:00:00
abstract::Studying the effects of microbial volatile organic compounds (VOCs) on plant growth is challenging because the production of volatiles depends on many environmental factors. Adding to this complexity, the method of volatile exposure itself can lead to different responses in plants and may account for some of the contr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1104-5
更新日期:2015-06-01 00:00:00
abstract::The induction of the enzyme activities involved in butane metabolism in Pseudomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Cells grown in each of the butane metabolites readily consumed the growth substrate and downs...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100304
更新日期:2001-07-01 00:00:00
abstract::Development of a transformation system in the n-alkane-assimilating diploid yeast Candida tropicalis requires an antibiotic resistance gene in order to establish a selectable marker. The resistance gene for hygromycin B has often been used as a selectable marker in yeast transformation. However, C. tropicalis harborin...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002039900125
更新日期:2000-03-01 00:00:00
abstract::Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato (B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurre...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1350-9
更新日期:2017-07-01 00:00:00
abstract::Some unicellular N(2)-fixing cyanobacteria have recently been found to lack a functional photosystem II of photosynthesis. Such organisms, provisionally termed UCYN-A, of the oceanic picoplanktion are major contributors to the global marine N-input by N(2)-fixation. Since their photosystem II is inactive, they can per...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-010-0621-5
更新日期:2010-10-01 00:00:00
abstract::Outer membrane vesicles (OMVs) are released from the outer membrane of Gram-negative bacteria. Moreover, Gram-positive bacteria also produce membrane-derived vesicles. As OMVs transport several bacterial components, especially from the cell envelope, their interaction with the host cell, with other bacteria or as immu...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-014-1042-7
更新日期:2015-01-01 00:00:00
abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00411361
更新日期:1979-08-06 00:00:00
abstract::P1B-type ATPases are involved in heavy metal transport across the plasma membrane. Some Mycobacterium tuberculosis P-type ATPases are induced during infection, suggesting that this type of transporter could play a critical role in mycobacterial survival. To date, the ion specificity of M. tuberculosis heavy metal-tran...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1465-z
更新日期:2018-04-01 00:00:00
abstract::TonB-dependent receptors in concert with the TonB-ExbB-ExbD protein complex are responsible for the uptake of iron and substances such as vitamin B12 in several bacterial species. In this study, Tn5 mutagenesis of the sugarcane endophytic bacterium Gluconacetobacter diazotrophicus led to the isolation of a mutant with...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-1045-4
更新日期:2015-03-01 00:00:00
abstract::In Lactobacillus casei BL23, phosphofructokinase activity was higher in cells utilizing sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). The phosphofructokinase gene (pfk) was cloned from L. casei and shown to be clustered with the gene encoding pyruvate kinase (pyk). pfk and pyk ge...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0003-6
更新日期:2005-09-01 00:00:00
abstract::Selenium nanoparticles (Se NPs) were synthesized using Saccharomyces cerevisiae yeast. Influences of different amounts of sodium selenite (5.0, 10.0, 15.0, 20.0, and 25 µg) were evaluated on growth of yeast during incubation at 32 °C, during 4 days. UV-Vis spectroscopy results have shown that synthesized Se NPs had br...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01831-0
更新日期:2020-07-01 00:00:00
abstract::The induction by D,L-nicotine of the enantiozymes 6-hydroxy-L-nicotine oxidase and 6-hydroxy-D-nicotine oxidase in Arthrobacter oxidans was differently affected by the inhibitors of Escherichia coli gyrase, novobiocin and nalidixic acid. These compounds inhibited 6-hydroxy-L-nicotine oxidase induction slightly, but le...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00521289
更新日期:1982-12-03 00:00:00
abstract::Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was fo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000160
更新日期:2000-05-01 00:00:00
abstract::The halophilic archaeal strain GX71(T) was isolated from the Gangxi marine solar saltern near the Weihai city of Shandong Province, China. Cells of the strain were pleomorphic and lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. Strain GX71(T) was able to grow at 25-45 °C (optimum 30 ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-014-0975-1
更新日期:2014-06-01 00:00:00
abstract::In yeasts, several sensing systems localized to the plasma membrane which transduce information regarding the availability and quality of nitrogen and carbon sources and work in parallel with the intracellular nutrient-sensing systems, regulate the expression and activity of proteins involved in nutrient uptake and ut...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0022-3
更新日期:2005-11-01 00:00:00
abstract::Anaerobic digestion, a recently hot technology to produce biogases especially methane generation for biofuel from wastewater, is considered an effective explanation for energy crisis and global pollution threat. A complex microbiome population is present in sludge, which plays an important role in the digestion of com...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01716-x
更新日期:2020-01-01 00:00:00
abstract::Pulicat Lake sediments are often severely polluted with the toxic heavy metal mercury. Several mercury-resistant strains of Bacillus species were isolated from the sediments and all the isolates exhibited broad spectrum resistance (resistance to both organic and inorganic mercuric compounds). Plasmid curing assay show...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0088-6
更新日期:2006-04-01 00:00:00
abstract::The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 micro...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413136
更新日期:1989-01-01 00:00:00
abstract::The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1222-8
更新日期:2016-09-01 00:00:00