Abstract:
:The determination of enzymatic activities in cell-free extracts of Acidaminococcus fermentans and Peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (R)-2-hydroxyglutarate to acetate and butyrate. From the ratio of these products the amount of ATP generated by substrate level phosphorylation was calculated. Growth experiments with the organisms including Clostridium symbiosum and Clostridium tetanomorphum indicated that a sodium gradient contributed additional energy for growth. The high growth yields found in organisms containing the biotin dependent sodium pump glutaconyl-CoA decarboxylase could be reduced by the sodium ionophor monensin. In P. asaccharolyticus energy equivalent up to 0.6 mol ATP per mol of glutaconyl-CoA decarboxylated was conserved via the Na+ gradient. The data may explain the growth promoting effects of monensin in cattle.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Wohlfarth G,Buckel Wdoi
10.1007/BF00447055subject
Has Abstractpub_date
1985-07-01 00:00:00pages
128-35issue
2eissn
0302-8933issn
1432-072Xjournal_volume
142pub_type
杂志文章abstract::Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the max...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050731
更新日期:1999-05-01 00:00:00
abstract::The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 micro...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413136
更新日期:1989-01-01 00:00:00
abstract::The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406170
更新日期:1980-12-01 00:00:00
abstract::The molar percentages of guanine plus cytosine in the DNA of 51 strains of Simonsiellaceae were determined by buoyant density ultracentrifugation of cell lysates in CsCl. The DNA base ratios ranged from 41-55 mole-% guanine plus cytosine. These values fall within the range known for the Order Cytophagales, the non-fru...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492026
更新日期:1977-06-20 00:00:00
abstract::Geobacter species such as G. bremensis, G. pelophilus, and G. sulfurreducens are obligately anaerobic and grow in anoxic, non-reduced medium by fast reduction of soluble ferric citrate. In contrast, insoluble ferrihydrite was either only slowly or not reduced when supplied as electron acceptor in similar growth experi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0686-0
更新日期:2004-10-01 00:00:00
abstract::1. Culture filtrates of heterotrophic bacteria were tested for their stimulatory effect on nitrification of three strains of Nitrobacter. 2. Yeast extract-peptone solution, in which Pseudomonas fluorescens had grown, after removal of the cells was added to autotrophically growing cultures of Nitrobacter agilis; it cau...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454856
更新日期:1976-07-01 00:00:00
abstract::Several studies have investigated the effects of ionizing and non-ionizing radiations on microorganisms. However, the interaction between the magnetic field radiations and bacteria is less studied. The aim of our study was to study the effect of static magnetic field on the biofilm formation in Pseudomonas aeruginosa ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01719-8
更新日期:2020-01-01 00:00:00
abstract::Periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. Sequential colonization of a broad range of bacteria, including Fusobacterium nucleatum and Porphyromonas gingivalis, is an important phenomenon in this disease mode...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1223-7
更新日期:2016-08-01 00:00:00
abstract::The induction of the enzyme activities involved in butane metabolism in Pseudomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Cells grown in each of the butane metabolites readily consumed the growth substrate and downs...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100304
更新日期:2001-07-01 00:00:00
abstract::Inactivation of peroxisomal enzymes in the yeast Hansenula polymorpha was studied following transfer of cells into cultivation media in which their activity was no longer required for growth. After transfer of methanol-grown cells into media containing glucose - a substrate that fully represses alcohol oxidase synthes...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00407757
更新日期:1983-06-01 00:00:00
abstract::A thermostable homodimeric isocitrate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was purified and characterized. The mol. mass of the isocitrate dehydrogenase subunit was 42 kDa as determined by SDS-PAGE. Following separation by SDS-PAGE, A. fulgidus isocitrate dehydrogen...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050516
更新日期:1997-11-01 00:00:00
abstract::Histoplasma capsulatum yeast cells have been studied by immunoelectron microscopy using rabbit polyclonal antisera and a biotin-avidin-peroxidase detection system. An antigenic surface layer has been visualized in the cell wall of immunostained organisms. This layer was not seen in samples prepared by standard electro...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00693397
更新日期:1985-08-01 00:00:00
abstract::The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was de...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0533-4
更新日期:2010-02-01 00:00:00
abstract::AMP-degrading pathways in Azotobacter vinelandii cells were investigated. AMP nucleosidase (EC 3.2.2.4) was rapidly synthesized and reached a maximum at 24 h, while the activity of 5'-nucleotidase (EC 3.1.3.5) specific for AMP, which was negligible during the logarithmic phase of the growth, first appeared in 24 h-cul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406162
更新日期:1980-12-01 00:00:00
abstract::Polysomes were isolated both from growing gametophytes of Allomyces arbuscula and from gametangia prepared from mycelia at different periods during gametogenesis. Analysis of polysomes by sucrose gradients showed that ribosomes present in the gametangia monosome pool were shifted into polysomes. This shift was found t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413089
更新日期:1977-04-01 00:00:00
abstract::The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0865-3
更新日期:2013-03-01 00:00:00
abstract::A high-efficiency fluoranthene-degrading bacterium Paenibacillus sp. PRNK-6 was isolated from PAH-contaminated soil. The strain degrades 96% (240 mg l-1) of fluoranthene in 48 h. Various metabolic intermediates of fluoranthene catabolism were identified by gas chromatography (GC) and gas chromatography-high resolution...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1431-9
更新日期:2018-01-01 00:00:00
abstract::Streptococcus intermedius is a causative agent of brain or liver abscesses. S. intermedius produces intermedilysin that plays a pivotal role in pathogenicity. We identified other pathogenic factors and described a fibronectin binding protein (FBP) homolog of S. intermedius (FbpI) that mediated bacterial adhesion to ep...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01922-y
更新日期:2020-10-01 00:00:00
abstract::Acinetobacter strain IVS-B aerobically grows on isovalerate as sole carbon and energy source. Isovalerate is metabolised via isovaleryl-CoA, an intermediate of the oxidative (S)-leucine degradation pathway. A 3-methylglutaconyl-CoA hydratase (EC 4.2.1.18) was purified 65-fold to apparent homogeneity from cell-free ext...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0095-7
更新日期:2006-05-01 00:00:00
abstract::A Gram-stain-negative, aerobic, non-motile and yellow-colored bacterium, strain 17J57-3 T, was isolated from soil collected in Pyeongchang city, Korea. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 17J57-3 T formed a distinct lineage within the family Oxalobacteraceae (order Burkholderial...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02076-7
更新日期:2020-10-15 00:00:00
abstract::Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1420-z
更新日期:2017-12-01 00:00:00
abstract::The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00428588
更新日期:1977-05-13 00:00:00
abstract::Anaplerotic fixation of carbon dioxide by the fungus Aspergillus nidulans when grown under carbon-limited conditions was mediated by pyruvate carboxylase and a phosphoenol pyruvate (PEP)-metabolising enzyme which has been tentatively designated as PEP carboxylase. The activities of both enzymes were growth rate depend...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00422531
更新日期:1981-01-01 00:00:00
abstract::A complex polydispersity became apparent when the poly(glycerophosphate) lipoteichoic acid of Enterococcus faecalis was chromatographed on DEAE-Sephadex. The chain length varied between 13 and 33 glycerophosphate residues per lipid anchor. In parallel, the extent of chain glycosylation increased from 0.2 to 0.4 digluc...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00249103
更新日期:1992-01-01 00:00:00
abstract::Nitrosomonas europaea, a Gram-negative obligate chemolithoautotroph, participates in global nitrogen cycling by carrying out nitrification and derives energy for growth through oxidation of ammonia. In this work, the physiological, proteomic, and transcriptional responses of N. europaea to zinc stress were studied. Th...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0341-7
更新日期:2008-06-01 00:00:00
abstract::The chb operon of Escherichia coli is involved in the utilization of chitooligosaccharides. While acquisition of two classes of mutations leading to altered regulation of the chb operon is necessary to confer the ability to utilize the glucose disaccharide cellobiose to wild-type strains of E. coli, in the closely rel...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1299-0
更新日期:2017-03-01 00:00:00
abstract::Synechococcus elongatus strain PCC7942 cells were grown in high or low environmental concentrations of inorganic C (high-C(i), low-C(i)) and subjected to a light shift from 50 micromol m(-2) s(-1) to 500 micromol m(-2) s(-1). We quantified photosynthetic reductant (O(2) evolution) and molar cellular contents of phycob...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0761-1
更新日期:2005-03-01 00:00:00
abstract::Ever since the development of the first antibiotic compound with anticancer potential, researchers focused on isolation and characterization of prospective microbial natural products with potential anti-infective and anticancer activities. The present work describes the production of bioactive metabolites by heterotro...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02106-4
更新日期:2020-11-02 00:00:00
abstract::Synthetic biology, the design and synthesis of synthetic biological systems from DNA to whole cells, has provided us with the ultimate tools for space exploration and colonisation. Herein, we explore some of the most significant advances and future prospects in the field of synthetic biology, in the context of astrobi...
journal_title:Archives of microbiology
pub_type: 信件
doi:10.1007/s00203-019-01651-x
更新日期:2019-08-01 00:00:00
abstract::In addition to rhizobia, other non-symbiotic endophytic bacteria also have been simultaneously isolated from the same root nodules. The existence of non-symbiotic endophytic bacteria in leguminous root nodules is a universal phenomenon. The vast majority of studies have detected endophytic bacteria in other plant tiss...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01752-7
更新日期:2020-03-01 00:00:00