Abstract：
:RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacteria, an Escherichia coli rpoS probe was constructed and used to detect the presence of this gene in different species. A gene homologous to rpoS was found in Citrobacter amalonaticus, Enterobacter cloacae, Klebsiella planticola, Kluyvera cryocrescens, Serratia rubidaea, Shigella sonnei, and Yersinia ruckeri. Providencia stuartii and Proteus vulgaris were the only tested enterobacteria that did not show any signal with the E. coli rpoS probe or that did not lead to amplification of an rpoS fragment using specific primers. The rpoS gene from E. cloacae and from K. cryocrescens was cloned and sequenced and a mutant allele was constructed in E. cloacae. Survival rates under different harsh conditions were followed in order to determine the effect of rpoS inactivation in exponential- and stationary-phase cells of both strains. E. cloacae rpoS mutants were more sensitive to extreme pH, high osmolarity, and high temperature than the wild-type.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Martínez-García E,Tormo A,Navarro-Llorens JMdoi
10.1007/s002030100277keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
395-404issue
6eissn
0302-8933issn
1432-072Xjournal_volume
175pub_type
杂志文章abstract：:Denitrification by Thiobacillus denitrificans "RT" strain was investigated using manometry and gas chromatography. 1. From nitrate, resting cells produced only nitrogen anaerobically with thiosulfate as the electron donor. The data suggest that nitrate was assimilated and dissimilated by the same nitrate reductase, as...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436326
更新日期：1975-03-12 00:00:00
abstract：:The endophytic diazotrophic Gluconacetobacter diazotrophicus PAL5 was originally isolated from sugarcane (Saccharum officinarum). The biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0794-1
更新日期：2012-07-01 00:00:00
abstract：:We report the phylogenetic and physiological characterization of a mesophilic and halophilic member of the filamentous anoxygenic phototrophic (FAP) bacteria, provisionally named ' Candidatus Chorothrix halophila' gen. nov. sp. nov., that has been maintained in a highly enriched culture in our laboratory for over a de...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0615-7
更新日期：2004-01-01 00:00:00
abstract：:The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406155
更新日期：1980-12-01 00:00:00
abstract：:A monoclonal antibody obtained by immunization of mice with heat-killed cells of Listeria monocytogenes serotype 4d showed reactivity towards a protein (P45) from L. monocytogenes with an apparent molecular mass of 45 kDa. This protein was detected in the culture supernatant and at the cell surface of L. monocytogenes...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050003
更新日期：2000-01-01 00:00:00
abstract：:Outer membrane vesicles (OMVs) are released from the outer membrane of Gram-negative bacteria. Moreover, Gram-positive bacteria also produce membrane-derived vesicles. As OMVs transport several bacterial components, especially from the cell envelope, their interaction with the host cell, with other bacteria or as immu...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-014-1042-7
更新日期：2015-01-01 00:00:00
abstract：:In this study, Sorangium cellulosum So ce56 was phenotypically and genotypically analysed in order to evaluate whether this strain can be used in a comprehensive genome project as a representative of the secondary metabolite-producing myxobacteria. In contrast to many other strains of S. cellulosum, strain So ce56 was...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0479-2
更新日期：2002-12-01 00:00:00
abstract：:During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02122-4
更新日期：2021-01-02 00:00:00
abstract：:The TTA codon, one of the six available codons for the amino acid leucine, is the rarest codon among the high GC genomes of Actinobacteria including Frankia. This codon has been implicated in various regulatory mechanisms involving secondary metabolism and morphological development. TTA-mediated gene regulation is wel...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0731-8
更新日期：2012-01-01 00:00:00
abstract：:Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1420-z
更新日期：2017-12-01 00:00:00
abstract：:The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050765
更新日期：1999-10-01 00:00:00
abstract：:Photosynthesis was measured by the 14C method on natural as well as low light adapted populations of Chloroflexus (a photosynthetic bacterium) and Synechococcus (a blue-green alga) from hot springs in Yellowstone National Park (Wyoming U.S.A.), to test the ability of these phototrophs to photosynthesize at a variety o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428590
更新日期：1977-05-13 00:00:00
abstract：:Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01618-y
更新日期：2019-03-01 00:00:00
abstract：:Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01999-5
更新日期：2021-01-01 00:00:00
abstract：:Pyruvate kinase (ATP: pyruvate phosphotransferase (EC 2.7.1.40) was partially purified from both autotrophically and heterotrophically grown Paracoccus denitrificans. The organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. The enzyme isolated from b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427847
更新日期：1977-10-24 00:00:00
abstract：:Mesorhizobium tianshanense is a nitrogen-fixing bacterium that can establish symbiotic associations with Glycyrrhiza uralensis in the form of root nodules. Nodule formation in rhizobia often requires various secreted carbohydrates. To investigate exopolysaccharide (EPS) production and function in M. tianshanense, we p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0345-3
更新日期：2008-05-01 00:00:00
abstract：:A 2.3-L bioreactor was specially adapted to grow hyperthermophilic microorganisms under controlled conditions of temperature, pH, redox potential and dissolved O(2). Using this bioreactor regulated at 80°C and pH 7.0, we demonstrated that Thermotoga maritima recovered its growth despite being exposed to oxygen for a s...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0687-8
更新日期：2011-06-01 00:00:00
abstract：:Non-hemolytic enterotoxin (NHE), a tri-partite, proteinaceous toxin encoded by contiguous nheA, nheB and nheC genes of Bacillus cereus sensu lato (B. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. However, B. cereus s.l. includes a number of closely related strains, and the occurre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1350-9
更新日期：2017-07-01 00:00:00
abstract：:Rather recently it has become clear that prokaryotes (Archaea and Bacteria) are able to glycosylate proteins. A literature survey revealed the different types of glycoproteins. They include mainly surface layer (S-layer) proteins, flagellins, and polysaccharide-degrading enzymes. Only in a few cases is structural info...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050484
更新日期：1997-09-01 00:00:00
abstract：:The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1222-8
更新日期：2016-09-01 00:00:00
abstract：:Glutamine synthetase (GS) activity of Schizosaccharomyces pombe 972 was high in ammonia-limited cultures, low in phosphate- and sulphate-limited cultures and not detected in glucose-limited cultures. When ammonia was 'pulsed' into an ammonia-limited culture then GS activity decreased at a rate faster than that calcula...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00549365
更新日期：1977-01-11 00:00:00
abstract：:The presence of teichoic acids in a number of streptomycetes led to the conclusion that these biopolymers were widely spread in genus Streptomyces. The nature of the teichoic acid present in the mycelium was determined by extracting it with 10% trichloroacetic acid, precipitating it with ethanol and identifying the pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421893
更新日期：1980-05-01 00:00:00
abstract：:NAD kinase was purified 180-fold from Bacillus licheniformis to determine the role it plays in NADP turnover in this organism. The enzyme was found to have a pH optimum of 6.8 and an apparent Km for NAD of 2.7 mM. The ATP saturation curve was not hyperbolic; 5.5 mM ATP was required to reach half maximal activity. Both...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409878
更新日期：1986-05-01 00:00:00
abstract：:We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca(2+)-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0498-3
更新日期：2009-09-01 00:00:00
abstract：:The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00381781
更新日期：1995-02-01 00:00:00
abstract：:The presence of one periplasmic [NiFe] hydrogenase, one periplasmic [Fe] hydrogenase, and one cytoplasmic NADP-reducing hydrogenase has been previously established in Desulfovibrio fructosovorans. In the present work, marker-exchange mutagenesis was performed to determine the function of the tetrameric NADP-reducing h...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050414
更新日期：1997-01-01 00:00:00
abstract：:The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050777
更新日期：1999-12-01 00:00:00
abstract：:Dunaliella tertiolecta (marine) and D. viridis (halophilic) were each trained by serial transfer to grow at salt concentrations previously regarded as the other's domain. D. viridis then had a salt optimum at 1.0-1.5 M sodium chloride whereas that for D. tertiolecta was less than 0-2 M. Nevertheless D. tertiolecta gre...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428592
更新日期：1977-05-13 00:00:00
abstract：:Pure cultures of an aerobically grown Flavobacterium sp. were shown by hydride generation-cold trap-atomic absorption spectrometry to biomethylate inorganic antimony (III) supplied as potassium antimony tartrate. Growth inhibition of the Flavobacterium sp. by antimony (III) over the range 0-30 mg Sb l(-1) was assessed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0456-9
更新日期：2002-10-01 00:00:00
abstract：:Anguibactin, an important virulent factor in Vibrio anguillarum serotype O1, is synthesized by a nonribosomal peptide synthetases (NRPS) system encoded on a 65-kb virulence plasmid pJM1. angE, as one of the NRPS genes, is responsible for selecting and activating 2,3-dihydroxybenzoic acid (2,3-DHBA), an important precu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0652-x
更新日期：2004-04-01 00:00:00
