Insights from genome of Clostridium butyricum INCQS635 reveal mechanisms to convert complex sugars for biofuel production.


:Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based on protein-conserved domains revealed a higher diversity of glycoside hydrolases than previously found in C. acetobutylicum ATCC824 strain. More than 30 glycoside hydrolases (GH) families were found; families of GH involved in degradation of galactan, cellulose, starch and chitin were identified as most abundant (close to 50 % of all sequences assigned as GH) in C. butyricum INCQS635. KEGG metabolic pathways reconstruction allowed us to verify possible routes in the C. butyricum INCQS635 and C. acetobutylicum ATCC824 genomes. Metabolic pathways for ethanol synthesis are similar for both species, but alcohol dehydrogenase of C. butyricum INCQS635 and C. acetobutylicum ATCC824 was different. The genomic repertoire of C. butyricum is an important resource to underpin future studies towards improved solvents production.


Arch Microbiol


Archives of microbiology


Bruce T,Leite FG,Miranda M,Thompson CC,Pereira N Jr,Faber M,Thompson FL




Has Abstract


2016-03-01 00:00:00














  • Cold survival strategies for bacteria, recent advancement and potential industrial applications.

    abstract::Microorganisms have evolved themselves to thrive under various extreme environmental conditions such as extremely high or low temperature, alkalinity, and salinity. These microorganisms adapted several metabolic processes to survive and reproduce efficiently under such extreme environments. As the major proportion of ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章,评审


    authors: Dhaulaniya AS,Balan B,Kumar M,Agrawal PK,Singh DK

    更新日期:2019-01-01 00:00:00

  • [Diurnal progress of NADP-linked glyceraldehyde-3-phosphate-dehydrogenase in synchronous culture of unicellular green alga Ankistrodesmus braunii and its susceptibility to X-irradiation and inhibitiors of protein synthesis].

    abstract::1. The daily progress of NADP-linked GPD-activity rise in synchronous culture of Ankistrodesmus braunii was investigated in respect to short time increase of activity by light. After various exposure times cells were temporarily deprived of light and subsequently the so-called dark value as well as the light value (da...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Theiss-Seuberling HB

    更新日期:1975-06-22 00:00:00

  • Purification, characterization and gene cloning of isoeugenol-degrading enzyme from Pseudomonas putida IE27.

    abstract::An isoeugenol-degrading enzyme was purified to homogeneity from Pseudomonas putida IE27, an isoeugenol-assimilating bacterium. The purified enzyme was a 55 kDa monomer and catalyzed the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Another...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Yamada M,Okada Y,Yoshida T,Nagasawa T

    更新日期:2007-06-01 00:00:00

  • Evidence for an arene oxide-NIH shift pathway in the transformation of naphthalene to 1-naphthol by Bacillus cereus.

    abstract::Bacillus cereus ATCC 14579 transformed naphthalene predominately to 1-naphthol. Experiments with [14C]naphthalene showed that over a 24 h period, B. cereus oxidized 5.2% of the added naphthalene. 1-Naphthol accounted for approximately 80% of the total metabolites. B. cereus incubated with naphthalene under the presenc...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Cerniglia CE,Freeman JP,Evans FE

    更新日期:1984-08-01 00:00:00

  • The role of the fatty acid beta-oxidation multienzyme complex from Pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadBA operon from P. oleovorans and of the enoyl-CoA hydratase genes phaJ from P. oleovorans

    abstract::In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Fiedler S,Steinbüchel A,Rehm BH

    更新日期:2002-08-01 00:00:00

  • Thermococcus waiotapuensis sp. nov., an extremely thermophilic archaeon isolated from a freshwater hot spring.

    abstract::An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: González JM,Sheckells D,Viebahn M,Krupatkina D,Borges KM,Robb FT

    更新日期:1999-08-01 00:00:00

  • Role of carbon dioxide in germination of spores of Streptomyces viridochromogenes.

    abstract::CO2 is required continuously during germination of Streptomyces viridochromogenes spores. Spores incubated in a defined germination medium in the absence of CO2 remain phase bright and do not release spore carbon. In the presence of CO2, the spores initiate germination accompanied by loss of refractility and spore car...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Grund AD,Ensign JC

    更新日期:1978-09-01 00:00:00

  • In situ growth of the novel SM1 euryarchaeon from a string-of-pearls-like microbial community in its cold biotope, its physical separation and insights into its structure and physiology.

    abstract::Recently, a unique archaeal/bacterial community that grows in a macroscopically visible string-of-pearls-like structure in cold (~10 degrees C), sulfurous marsh water was discovered. Here, a new technique is described that allows the fast and reliable growth of these string-of-pearls-like microbial communities in larg...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Moissl C,Rudolph C,Rachel R,Koch M,Huber R

    更新日期:2003-09-01 00:00:00

  • Diaphorobacter ruginosibacter sp. nov., isolated from soybean root nodule, and emended description of the genus Diaphorobacter.

    abstract::A gram-negative bacterium designated BN30(T), which is motile with a polar flagellum, non-endospores forming, oxidase- and catalase-positive, was isolated from soybean root nodule. The organism is facultative anaerobic and surface-wrinkled rod. It can grow at 10-40 °C, pH 6-8 and 6 % (w/v) NaCl. BLASTn search based on...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Wei XL,Han MS,Xia CC,Ding SL,Xu L,Lin YB,Wang L,Wei GH

    更新日期:2015-06-01 00:00:00

  • Cloning and characterization of a gene encoding the gamma-butyrolactone autoregulator receptor from Streptomyces clavuligerus.

    abstract::With primers designed for the conserved region of the gamma-butyrolactone autoregulator receptor proteins from Streptomyces species, PCR using the Streptomyces clavuligerus genome DNA as a template gave a clear band of 100 bp, the sequence of which revealed high similarity to the expected region of a receptor gene. By...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Kim HS,Lee YJ,Lee CK,Choi SU,Yeo SH,Hwang YI,Yu TS,Kinoshita H,Nihira T

    更新日期:2004-09-01 00:00:00

  • The use of stable sulfur isotope labelling to elucidate sulfur metabolism by Clostridium pasteurianum.

    abstract::An unique isotope labelling experiment was conducted whereby mixtures of sulfate and sulfite of different isotopic compositions were metabolized by Clostridium pasteurianum. The results showed during reduction of 1 mM SO3= plus 1 mM SO4=, essentially all evolved H2S arose from the sulfite whereas in the case of cellul...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: McCready RG,Laishley EJ,Krouse HR

    更新日期:1976-09-01 00:00:00

  • Expression of the bifunctional Bacillus subtilis TatAd protein in Escherichia coli reveals distinct TatA/B-family and TatB-specific domains.

    abstract::In the Tat protein export pathway of Gram-negative bacteria, TatA and TatB are homologous proteins that carry out distinct and essential functions in separate sub-complexes. In contrast, Gram-positive Tat systems usually lack TatB and the TatA protein is bifunctional. We have used a mutagenesis approach to delineate T...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Barnett JP,Lawrence J,Mendel S,Robinson C

    更新日期:2011-08-01 00:00:00

  • Role of respiration and glutathione in cadmium-induced oxidative stress in Escherichia coli K-12.

    abstract::Cadmium is a widespread pollutant that has been associated with oxidative stress, but the mechanism behind this effect in prokaryotes is still unclear. In this work, we exposed two glutathione deficient mutants (DeltagshA and DeltagshB) and one respiration deficient mutant (DeltaubiE) to a sublethal concentration of c...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Pacheco CC,Passos JF,Castro AR,Moradas-Ferreira P,De Marco P

    更新日期:2008-03-01 00:00:00

  • Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA.

    abstract::Clostridium aminobutyricum ferments 4-aminobutyrate via succinic semialdehyde, 4-hydroxybutyrate, 4-hydroxybutyryl-CoA and crotonyl-CoA to acetate and butyrate. The genes coding for the enzymes that catalyse the interconversion of these intermediates are arranged in the order abfD (4-hydroxybutyryl-CoA dehydratase), a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Gerhardt A,Cinkaya I,Linder D,Huisman G,Buckel W

    更新日期:2000-09-01 00:00:00

  • Xylose and some non-sugar carbon sources cause catabolite repression in Saccharomyces cerevisiae.

    abstract::Glucose and other sugars, such as galactose or maltose, are able to cause carbon catabolite repression in Saccharomyces cerevisiae. Although glycolytic intermediates have been suggested as signal for repression, no evidence for such a control mechanism is available. The establishment of a correlation between levels of...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Belinchón MM,Gancedo JM

    更新日期:2003-10-01 00:00:00

  • Introduction of a novel pathway for IAA biosynthesis to rhizobia alters vetch root nodule development.

    abstract::We introduced into Rhizobium leguminosarum bv. viciae LPR1105 a new pathway for the biosynthesis of the auxin, indole-3-acetic acid (IAA), under the control of a stationary phase-activated promoter active both in free-living bacteria and bacteroids. The newly introduced genes are the iaaM gene from Pseudomonas savasta...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Camerini S,Senatore B,Lonardo E,Imperlini E,Bianco C,Moschetti G,Rotino GL,Campion B,Defez R

    更新日期:2008-07-01 00:00:00

  • Diversity and evolution of Lactobacillus casei group isolated from fermented dairy products in Tibet.

    abstract::Bacteria in Lactobacillus casei group, including Lactobacillus casei (L. casei), Lactobacillus paracasei (L. paracasei), and Lactobacillus rhamnosus (L. rhamnosus) are important lactic acid bacteria in the production of fermented dairy products and are faced with the controversial nomenclatural status due to their clo...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Feng J,Jiang Y,Li M,Zhao S,Zhang Y,Li X,Wang H,Lin G,Wang H,Li T,Man C

    更新日期:2018-09-01 00:00:00

  • Purification and characterization of a methanol-induced cobamide-containing protein from Sporomusa ovata.

    abstract::The major cobamide-containing protein from methanol-utilizing Sporomusa ovata was 8-fold enriched to apparent homogeneity. The protein exhibited a molecular mass of 40 kDa and of 38 kDa determined by gel filtration and by SDS-polyacrylamide gel electrophoresis, respectively. This finding indicates a monomeric protein ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Stupperich E,Aulkemeyer P,Eckerskorn C

    更新日期:1992-01-01 00:00:00

  • Fish borne Edwardsiella tarda eha involved in the bacterial biofilm formation, hemolytic activity, adhesion capability and pathogenicity.

    abstract::Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 ha...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Hassan HA,Ding X,Zhang X,Zhu G

    更新日期:2020-05-01 00:00:00

  • Investigations of the pigments from Cytophaga johnsonae Cy jl. New flexirubin-type pigments.

    abstract::Besides carotenoids a complex of flexirubin-type pigments was isolated from the gliding bacterium Cytophaga johnsonae Cy jl and separated into 6 components, which partly containe chlorine. In spite of the fact that these components still consist of pigment mixtures, the gross structures of 18 new flexirubin-type pigme...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Achenbach H,Kohl W,Wachter W,Reichenbach H

    更新日期:1978-06-26 00:00:00

  • Free tryptophan pool and tryptophan biosynthetic enzymes in Saccharomyces cerevisiae.

    abstract::The free tryptophan pool and the levels of two enzymes of tryptophan biosynthesis (anthranilate synthase and indoleglycerolphosphate synthase)have been determined in a wild type strain of Saccharomyces cerevisiae and in mutants with altered regulatory properties. ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Fantes PA,Roberts LM,Huetter R

    更新日期:1976-03-19 00:00:00

  • Ethanol-sensitive mutants of Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Aguilera A,Benítez T

    更新日期:1986-01-01 00:00:00

  • Poly-3-hydroxybutyrate production by washed cells of Alcaligenes eutrophus; purification, characterisation and potential regulatory role of citrate synthase.

    abstract::Washed cells prepared from carbon-limited continuous cultures of Alcaligenes eutrophus synthesised poly-3-hydroxybutyrate (PHB) rapidly when supplied with glucose, DL-lactate or L-lactate. Unlike growing cultures, washed cells excreted significant amounts of pyruvate. The combined rates of PHB production (qPHB) and py...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Henderson RA,Jones CW

    更新日期:1997-12-01 00:00:00

  • Genome information management and integrated data analysis with HaloLex.

    abstract::HaloLex is a software system for the central management, integration, curation, and web-based visualization of genomic and other -omics data for any given microorganism. The system has been employed for the manual curation of three haloarchaeal genomes, namely Halobacterium salinarum (strain R1), Natronomonas pharaoni...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Pfeiffer F,Broicher A,Gillich T,Klee K,Mejía J,Rampp M,Oesterhelt D

    更新日期:2008-09-01 00:00:00

  • Status (on/off) of oipA gene: their associations with gastritis and gastric cancer and geographic origins.

    abstract::Virulence factors of H. pylori, such as outer inflammatory protein A (oipA), are closely involved in the development of gastric diseases such as chronic gastritis and gastric cancer. The functional status of oipA is regulated by a repair mechanism based on CT dinucleotide repeats that influence the reading frame, thus...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Sallas ML,Dos Santos MP,Orcini WA,David ÉB,Peruquetti RL,Payão SLM,Rasmussen LT

    更新日期:2019-01-01 00:00:00

  • Enzymic arrangement and allosteric regulation of the aromatic amino acid pathway in Neisseria gonorrhoeae.

    abstract::The pathway construction and allosteric regulation of phenylalanine and tyrosine biosynthesis was examined in Neisseria gonorrhoeae. A single 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase enzyme sensitive to feedback inhibition by L-phenylalanine was found. Chorismate mutase and prephenate dehydratase ap...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Berry A,Jensen RA,Hendry AT

    更新日期:1987-01-01 00:00:00

  • Heterogeneity of lipoteichoic acid detected by anion exchange chromatography.

    abstract::A complex polydispersity became apparent when the poly(glycerophosphate) lipoteichoic acid of Enterococcus faecalis was chromatographed on DEAE-Sephadex. The chain length varied between 13 and 33 glycerophosphate residues per lipid anchor. In parallel, the extent of chain glycosylation increased from 0.2 to 0.4 digluc...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Leopold K,Fischer W

    更新日期:1992-01-01 00:00:00

  • Production of antibiotic carbomycin from Streptomyces graminofaciens with high lipid content mutation.

    abstract::The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Arafat HH,Abu-Tahon MA,Isaac GS

    更新日期:2020-10-19 00:00:00

  • Identification of the mycothiol synthase gene (mshD) encoding the acetyltransferase producing mycothiol in actinomycetes.

    abstract::Mycothiol is the predominant thiol in most actinomycetes, including Mycobacterium tuberculosis, and appears to play a role analogous to glutathione, which is not found in these bacteria. The enzymes involved in mycothiol biosynthesis are of interest as potential targets for new drugs directed against tuberculosis. In ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Koledin T,Newton GL,Fahey RC

    更新日期:2002-11-01 00:00:00

  • The role of calcium in stalk development and in phosphate acquisition in Caulobacter crescentus.

    abstract::Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Cal...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Poindexter JS

    更新日期:1984-06-01 00:00:00