Abstract:
:Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based on protein-conserved domains revealed a higher diversity of glycoside hydrolases than previously found in C. acetobutylicum ATCC824 strain. More than 30 glycoside hydrolases (GH) families were found; families of GH involved in degradation of galactan, cellulose, starch and chitin were identified as most abundant (close to 50 % of all sequences assigned as GH) in C. butyricum INCQS635. KEGG metabolic pathways reconstruction allowed us to verify possible routes in the C. butyricum INCQS635 and C. acetobutylicum ATCC824 genomes. Metabolic pathways for ethanol synthesis are similar for both species, but alcohol dehydrogenase of C. butyricum INCQS635 and C. acetobutylicum ATCC824 was different. The genomic repertoire of C. butyricum is an important resource to underpin future studies towards improved solvents production.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Bruce T,Leite FG,Miranda M,Thompson CC,Pereira N Jr,Faber M,Thompson FLdoi
10.1007/s00203-015-1166-4subject
Has Abstractpub_date
2016-03-01 00:00:00pages
115-27issue
2eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-015-1166-4journal_volume
198pub_type
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