Insights from genome of Clostridium butyricum INCQS635 reveal mechanisms to convert complex sugars for biofuel production.


:Clostridium butyricum is widely used to produce organic solvents such as ethanol, butanol and acetone. We sequenced the entire genome of C. butyricum INCQS635 by using Ion Torrent technology. We found a high contribution of sequences assigned for carbohydrate subsystems (15-20 % of known sequences). Annotation based on protein-conserved domains revealed a higher diversity of glycoside hydrolases than previously found in C. acetobutylicum ATCC824 strain. More than 30 glycoside hydrolases (GH) families were found; families of GH involved in degradation of galactan, cellulose, starch and chitin were identified as most abundant (close to 50 % of all sequences assigned as GH) in C. butyricum INCQS635. KEGG metabolic pathways reconstruction allowed us to verify possible routes in the C. butyricum INCQS635 and C. acetobutylicum ATCC824 genomes. Metabolic pathways for ethanol synthesis are similar for both species, but alcohol dehydrogenase of C. butyricum INCQS635 and C. acetobutylicum ATCC824 was different. The genomic repertoire of C. butyricum is an important resource to underpin future studies towards improved solvents production.


Arch Microbiol


Archives of microbiology


Bruce T,Leite FG,Miranda M,Thompson CC,Pereira N Jr,Faber M,Thompson FL




Has Abstract


2016-03-01 00:00:00














  • Specific protein phosphorylation induced in Xanthomonas campestris pv. oryzae by bacteriophage Xp12.

    abstract::We have investigated the endogenous phosphorylation patterns of phosphorylated proteins of Xanthomonas campestris pv. oryzae induced by its bacteriophages. For bacteriophage Xp12-infected cells, at least three phosphoproteins with apparent molecular weights of 28, 28.5 and 45 kDa were detected by in vitro labeling wit...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Cheng CM,Tu J,Yang CC,Kuo TT

    更新日期:1994-01-01 00:00:00

  • Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. II. The isolation and characterization of mutants auxotrophic for phenylalanine and tyrosine.

    abstract::1. Mutants derived from the hydrogen bacterium Alcaligenes eutrophus strain H 16 auxotrophic for phenylalanine and tyrosine were isolated employing mutagenic agents (EMS, nitrite), the colistine counterselection technique and the "pin-point" isolation method. Three different types of mutants were found: (1) Mutants, r...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Friedrich B,Schlegel HG

    更新日期:1975-04-07 00:00:00

  • Synthesis of beta-glucanase and other extracellular proteins by cells and protoplasts of the yeast Pichia polymorpha: effect of 2-deoxy-D-glucose.

    abstract::The synthesis of beta-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-D-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the prese...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Villa TG,Notario V,Villanueva JR

    更新日期:1975-11-07 00:00:00

  • Different assembly of acid and salt tolerance response in two dairy Listeria monocytogenes wild strains.

    abstract::A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Melo J,Andrew PW,Faleiro ML

    更新日期:2013-05-01 00:00:00

  • Oxygen uptake rates in the hyperthermophilic anaerobe Thermotoga maritima grown in a bioreactor under controlled oxygen exposure: clues to its defence strategy against oxidative stress.

    abstract::A 2.3-L bioreactor was specially adapted to grow hyperthermophilic microorganisms under controlled conditions of temperature, pH, redox potential and dissolved O(2). Using this bioreactor regulated at 80°C and pH 7.0, we demonstrated that Thermotoga maritima recovered its growth despite being exposed to oxygen for a s...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Lakhal R,Auria R,Davidson S,Ollivier B,Durand MC,Dolla A,Hamdi M,Combet-Blanc Y

    更新日期:2011-06-01 00:00:00

  • Characterization of the colistin (polymyxin E1 and E2) biosynthetic gene cluster.

    abstract::Colistin is a mixture of polymyxin E1 and E2, bactericidal pentacationic lipopeptides used to treat infections caused by Gram-negative pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae. Industrial production of colistin is obtained by a fermentation process of the natural producer Paenibacillus polymy...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Tambadou F,Caradec T,Gagez AL,Bonnet A,Sopéna V,Bridiau N,Thiéry V,Didelot S,Barthélémy C,Chevrot R

    更新日期:2015-05-01 00:00:00

  • A gas vesiculate planktonic strain of the purple non-sulfur bacterium Rhodoferax antarcticus isolated from Lake Fryxell, Dry Valleys, Antarctica.

    abstract::A moderately psychrophilic purple non-sulfur bacterium, Rhodoferax antarcticus strain Fryx1, is described. Strain Fryx1 was isolated from the water column under the ice of the permanently frozen Lake Fryxell, Antarctica. Cells of Fryx1 are long thin rods and contain gas vesicles, the first report of such structures in...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Jung DO,Achenbach LA,Karr EA,Takaichi S,Madigan MT

    更新日期:2004-10-01 00:00:00

  • Analysis of phenomena involved in the apical growth of Phycomyces blakesleeanus.

    abstract::The effects of the Ca(2+)/H(+) exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of Phycomyces blakesleeanus were analyzed. While all of the compou...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Ruiz-Herrera J,Martínez-Cadena G,Loarca F,Salcedo-Hernández R

    更新日期:2003-12-01 00:00:00

  • The role of the fatty acid beta-oxidation multienzyme complex from Pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadBA operon from P. oleovorans and of the enoyl-CoA hydratase genes phaJ from P. oleovorans

    abstract::In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Fiedler S,Steinbüchel A,Rehm BH

    更新日期:2002-08-01 00:00:00

  • Intra-amoebic localization of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii.

    abstract::Acanthamoeba castellanii is a free-living amoeba found mainly in humid environments and Arcobacter butzleri is an emerging zoonotic pathogen, both can establish in vitro endosymbiotic relationships in the absence of bacterial replication. We analyzed the localization of A. butzleri within A. castellanii establishing t...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Medina G,Leyán P,da Silva CV,Flores-Martin S,Manosalva C,Fernández H

    更新日期:2019-12-01 00:00:00

  • The molecular regulation of the reductive pentose phosphate pathway in Proteobacteria and Cyanobacteria.

    abstract::In phototrophic and chemoautotrophic proteobacteria, genes encoding enzymes of the Calvin-Benson-Bassham pathway of CO2 fixation are often found in clusters that are transcribed from a single promoter under control of the LysR-type transcriptional activator, CbbR. Mutations affecting CbbR prevent induction of cbb gene...

    journal_title:Archives of microbiology

    pub_type: 杂志文章,评审


    authors: Gibson JL,Tabita FR

    更新日期:1996-09-01 00:00:00

  • Effect of cra gene knockout together with edd and iclR genes knockout on the metabolism in Escherichia coli.

    abstract::To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Sarkar D,Siddiquee KA,Araúzo-Bravo MJ,Oba T,Shimizu K

    更新日期:2008-11-01 00:00:00

  • Intracellular localization of the particulate methane monooxygenase and methanol dehydrogenase in Methylomicrobium album BG8.

    abstract::The methanotrophic bacterium Methylomicrobium album BG8 uses methane as a sole source of carbon and energy. This bacterium forms an extensive intracytoplasmic membrane. The first enzymes of the methane oxidation pathway are the membrane-bound particulate methane monooxygenase and the periplasmic methanol dehydrogenase...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Brantner CA,Remsen CC,Owen HA,Buchholz LA,Perille Collins ML

    更新日期:2002-07-01 00:00:00

  • Analysis of the expression and function of the sigmaB-dependent general stress regulon of Bacillus subtilis during slow growth.

    abstract::Glucose-limited continuous cultures were used to analyze sigmaB activity at decreasing growth rates. Expression of the sigmaB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h-1, and both genes were induced approximately fivefold at a growth rate of 0.1 h-1 as compared to expression at the max...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Schweder T,Kolyschkow A,Völker U,Hecker M

    更新日期:1999-05-01 00:00:00

  • Studies on the macroconidia of Microsporum canis. Characteristics of in vitro amino acid incorporating system.

    abstract::The characteristics of an in vitro polyuridylic acid dependent amino acid incorporating system prepared from germinating macroconidia of Microsporum canis are described. The incorporation of 14C-phenylalanine into polyphenylalanine is dependent on S-30 extract, adenosine triphosphate, magnesium ions and polyuridylic a...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Jayaram BM,Rao GR

    更新日期:1979-01-16 00:00:00

  • Variations in the amino acid composition of cyanophycin in the cyanobacterium Synechocystis sp. PCC 6308 as a function of growth conditions.

    abstract::Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using pro...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Merritt MV,Sid SS,Mesh L,Allen MM

    更新日期:1994-01-01 00:00:00

  • Alanine dehydrogenase of the beta-lactam antibiotic producer Streptomyces clavuligerus.

    abstract::L-Alanine dehydrogenase was found in extracts of the antibiotic producer Streptomyces clavuligerus. The enzyme was induced by ammonia, and the level of induction was dependent on the extracellular concentraction. L-Alanine was the only amino acid able to induce alanine dehydrogenase. The enzyme was characterized from ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Aharonowitz Y,Friedrich CG

    更新日期:1980-03-01 00:00:00

  • Desulfitobacterium sp. strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols.

    abstract::A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Gerritse J,Renard V,Pedro Gomes TM,Lawson PA,Collins MD,Gottschal JC

    更新日期:1996-02-01 00:00:00

  • PknE, a serine/threonine protein kinase from Mycobacterium tuberculosis has a role in adaptive responses.

    abstract::Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Kumar D,Palaniyandi K,Challu VK,Kumar P,Narayanan S

    更新日期:2013-01-01 00:00:00

  • Temperature-sensitive loss of sexual agglutinability in Saccharomyces cerevisiae.

    abstract::Temperature dependency of sexual agglutination in Saccharomyces cerevisiae was found. Of 31 strains tested, which showed normal agglutination when cultured at 25 degrees C, 29 strains lost their sexual agglutinability when they were grown at 37 degrees C. ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Doi S,Yoshimura M

    更新日期:1977-09-28 00:00:00

  • Two class II D-tagatose-bisphosphate aldolases from enteric bacteria.

    abstract::Escherichia coli, Salmonella enterica, Klebsiella pneumoniaeand Klebsiella oxytocawere found to contain two D-tagatose 1,6-bisphosphate (TagBP)-specific aldolases involved in catabolism of galactitol (genes gatY gatZ) and of N-acetyl-galactosamine and D-galactosamine (genes kbaY kbaZ,also called agaY agaZ). The two al...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Brinkkötter A,Shakeri-Garakani A,Lengeler JW

    更新日期:2002-05-01 00:00:00

  • [Formation of bactericidal substances by Cosmarium impressulum (author's transl)].

    abstract::1. An already published test method for detecting bactericidal substances in paper chromatograms was further improved. 2. In cultures of Cosmarium impressulum free from bacteria, two bactericidal substances were found in the ether extracts from the algae and two others in the extracts of the culture medium. The are ac...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Söding H,Dörffling K,Mix M

    更新日期:1976-06-01 00:00:00

  • Extracellular arabinases in Aspergillus nidulans: the effect of different cre mutations on enzyme levels.

    abstract::The regulation of the syntheses of two arabinan-degrading extracellular enzymes and several intracellular L-arabinose catabolic enzymes was examined in wild-type and carbon catabolite derepressed mutants of Aspergillus nidulans. alpha-L-Arabinofuranosidase B, endoarabinase, L-arabinose reductase, L-arabitol dehydrogen...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: van der Veen P,Arst HN Jr,Flipphi MJ,Visser J

    更新日期:1994-01-01 00:00:00

  • Regulatory properties of the ADPglucose pyrophosphorylase from Rhodopseudomonas sphaeroides and from Rhodopseudomonas gelatinosa.

    abstract::The ADPglucose pyrophosphorylases from Rhodopseudomonas sphaeroides and Rhodopseudomonas gelatinosa are activated by fructose-6-phosphate, pyruvate and fructose-1,6 biophosphate-P2. The effects of the activators are to increase significantly the Vmax of ADPglucose synthesis and to lower the S0.5 values (concentration ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Preiss J,Greenberg E,Parsons TF,Downey J

    更新日期:1980-05-01 00:00:00

  • Two mechanisms for putrescine-dependent transcriptional expression of the putrescine aminotransferase gene, ygjG, in Escherichia coli.

    abstract::In this study, on evaluating the physiological function and mechanism of putrescine, we found that putrescine supplementation (1 mM) increases transcription of the putrescine aminotransferase gene, ygjG. Putrescine-dependent expression was confirmed by measuring β-galactosidase activity and with reverse transcription-...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Kim YS,Shin HC,Lee JH

    更新日期:2014-09-01 00:00:00

  • Evaluation of bacterial association in methane generation pathways of an anaerobic digesting sludge via metagenomic sequencing.

    abstract::Anaerobic digestion, a recently hot technology to produce biogases especially methane generation for biofuel from wastewater, is considered an effective explanation for energy crisis and global pollution threat. A complex microbiome population is present in sludge, which plays an important role in the digestion of com...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Ali N,Gong H,Liu X,Giwa AS,Wang K

    更新日期:2020-01-01 00:00:00

  • Characterization of a glucosylglycerol-phosphate-accumulating, salt-sensitive mutant of the cyanobacterium Synechocystis sp. strain PCC 6803.

    abstract::Salt-sensitive mutants of Synechocystis were obtained by random cartridge mutagenesis, and one mutant (mutant 4) was characterized in detail. The salt tolerance of mutant 4 was reduced to about 20% of that of the wild-type. This was caused by a defect in the biosynthetic pathway of the osmoprotective compound glucosyl...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Hagemann M,Richter S,Zuther E,Schoor A

    更新日期:1996-08-01 00:00:00

  • Growth of Candida utilis on ethanol and isopropanol.

    abstract::Candida utilis grew on ethanol and an ethanol-isopropanol-water (22:2:1 vols) mixture but not on isopropanol alone. Acetone accumulated in all cultures containing isopropranol but its presence in the alcohol mixture did not lower growth rate or yield significantly when compared with growth experiments on ethanol alone...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Prior B,Kilian S,Lategan P

    更新日期:1980-03-01 00:00:00

  • Alkaline lipase production by novel meso-tolerant psychrophilic Exiguobacterium sp. strain (AMBL-20) isolated from glacier of northeastern Pakistan.

    abstract::Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Yasin MT,Ali Y,Ahmad K,Ghani A,Amanat K,Basheir MM,Faheem M,Hussain S,Ahmad B,Hussain A,Bokhari SAI

    更新日期:2020-12-15 00:00:00

  • The early formation of the photosynthetic apparatus in Rhodospirillum rubrum.

    abstract::The time dependent assembly of the photosynthetic apparatus was studied in Rhodospirillum rubrum after transfer of cells growing aerobically in the dark to low aeration. While bacteriochlorophyll (Bchl) cellular levels increase continuously levels of soluble cytochrome C2 do not change significantly. Absorption spectr...

    journal_title:Archives of microbiology

    pub_type: 杂志文章


    authors: Oelze J,Pahike W

    更新日期:1976-07-01 00:00:00