Transfer and expression of the herbicide-degrading plasmid pJP4 in aerobic autotrophic bacteria.

Abstract:

:Plasmid pJP4 encoding the ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (Tfd+) was transferred by conjugation from Escherichia coli JMP397 to various lithoautotrophic strains of Alcaligenes eutrophus and to the autotrophic bacterium Pseudomonas oxalaticus. The herbicide-degrading function of the plasmid was phenotypically expressed in all of the recipients. The majority of Tfd+ transconjugants also exhibited additional plasmid-encoded properties such as 3-chlorobenzoate degradation, resistance to mercuric ions, and sensitivity to the male-specific bacteriophage PR11. Furthermore, Tfd+ transconjugants were able to act as donors of plasmid pJP4. Physical evidence is presented by agarose gel electrophoresis showing that plasmid pJP4 coexisted with the resident plasmids widely distributed in this group of bacteria. However, in some of the hosts plasmids pJP4 was not stably maintained, had a reduced size and tended to form multimers.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Friedrich B,Meyer M,Schlegel HG

doi

10.1007/BF00407938

subject

Has Abstract

pub_date

1983-02-01 00:00:00

pages

92-7

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

134

pub_type

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