Abstract：
:Klebsiella pneumoniae NCTC 418 was cultured aerobically in chemostat cultures (D = 0.3 h-1; 35 degrees C) under respectively carbon-, phosphate-, potassium-, sulphate-, and ammonia-limited conditions with glucose as the sole carbon and energy source. The effect of the external pH value on glucose metabolism and on the enzymes of the direct glucose oxidative pathway was examined. The pH value of the medium had a profound influence on both the activity and the synthesis of the glucose dehydrogenase and the gluconate dehydrogenase. At pH values ranging from pH 5.5 to pH 6.0 maximal activity and synthesis of these enzymes resulted in a more than 80% conversion of the glucose consumed into gluconate and 2-ketogluconate under potassium- or phosphate-limited conditions. On the other hand, no gluconate and/or 2-ketogluconate production could be detected when K. pneumoniae was cultured at pH 8.0. Whereas the synthesis of gluconate dehydrogenase seemingly was completely repressed, still some glucose dehydrogenase was present. The lack of glucose dehydrogenase activity at pH 8.0 was shown not to be due to the dissociation of the cofactor PQQ from the enzyme.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Hommes RW,Postma PW,Tempest DW,Neijssel OMdoi
10.1007/BF00413140subject
Has Abstractpub_date
1989-01-01 00:00:00pages
261-7issue
3eissn
0302-8933issn
1432-072Xjournal_volume
151pub_type
杂志文章abstract：:During cultivation of a gastric fungus, Coniochaeta polymorpha, growth of Nocardia colonies on top of the fungal culture raised the question whether bacteria originated from inside of fungus. In this study, the likelihood of intracellular origin of bacteria as well as interaction of two microorganisms was assessed. Fl...
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pub_type： 杂志文章
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journal_title：Archives of microbiology
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更新日期：1981-05-01 00:00:00
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更新日期：2017-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1997-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF00436326
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journal_title：Archives of microbiology
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doi：10.1007/BF00446842
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更新日期：1997-12-01 00:00:00
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更新日期：2014-10-01 00:00:00
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更新日期：1990-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1989-01-01 00:00:00
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更新日期：2003-08-01 00:00:00
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更新日期：2002-11-01 00:00:00
