The uptake of fructose by Pseudomonas putida.

Abstract:

:Fructose transport was not apparently affected in a number of Pseudomonas putida strains with deranged activity of a common glucose-gluconate uptake system, indicating the existence of an independent fructose uptake system. Fructose uptake by glucose-gluconate uptake mutants was induced by fructose and obeyed saturation kinetics (apparent Km equal 0.3 mM). The fructose uptake system serves to transport glucose in addition to fructose. The entry of fructose into P.putida cells appears to be mediated also by the glucose-gluconate uptake system, as shown by the ability to accumulate fructose of wild type cells grown on glucose, a substrate that induces the glucose-gluconate uptake system but not the fructose uptake system. In addition, fructose was found to be an inducer of the glucose-gluconate uptake system. The physiological significance of these observations is not clear because the fructose uptake system can provide the cell with a high enough internal concentration of fructose to support maximum growth rate on this hexose, as shown by following the growth course of a glucose-gluconate uptake mutants on fructose.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Vicente M

doi

10.1007/BF00428362

keywords:

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

163-6

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

102

pub_type

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