Abstract：
:We have investigated the endogenous phosphorylation patterns of phosphorylated proteins of Xanthomonas campestris pv. oryzae induced by its bacteriophages. For bacteriophage Xp12-infected cells, at least three phosphoproteins with apparent molecular weights of 28, 28.5 and 45 kDa were detected by in vitro labeling with [gamma-32P]-ATP. These Xp12-specific phosphoproteins only occurred with Xp12 infection, and were not shown in uninfected or Xp10-infected cells. The protein kinase(s) responsible could use either ATP or GTP as the nucleotide substrate with nearly the same efficiency. Magnesium was proved to be an essential factor for the phosphorylation. EGTA treatment excluding the possibility that the presumed protein kinase was calcium-dependent. Under our reaction conditions, the optimal phosphorylation occurred at pH 7 to 8, for 30 to 40 min at 25 to 37 degrees C. The Xp12-specific protein phosphorylation hint the existence of a physiological regulation mechanism involved in the life cycle of bacteriophage Xp12. Furthermore, the presumed protein kinase was shown to be encoded by the genome of Xp12 rather than indirectly induced by Xp12 infection.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Cheng CM,Tu J,Yang CC,Kuo TTdoi
10.1007/BF00303581subject
Has Abstractpub_date
1994-01-01 00:00:00pages
281-5issue
4eissn
0302-8933issn
1432-072Xjournal_volume
161pub_type
杂志文章abstract：:The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0366-6
更新日期：2008-07-01 00:00:00
abstract：:Cryptococcus adeliensis was initially described as a psycrophilic species containing a single strain CBS 8351(T) isolated from decayed algae in Terre Adelie (Antartida). Later, a second strain of this species was isolated from an immunosuppressed patient affected by leukaemia in Germany and recently several strains fr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0134-4
更新日期：2006-09-01 00:00:00
abstract：:Cadaverin was more readily incorporated than lysine into arthrobactin from Arthrobacter pascens and into ferrioxamin E from Streptomyces glaucescens. From a racemic mixture only the L-isomer of lysine is incorporated. The L-lysine decarboxylase activity was measured in vivo and in vitro. The enzyme from Arthrobacter p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402309
更新日期：1978-05-30 00:00:00
abstract：:In phototrophic and chemoautotrophic proteobacteria, genes encoding enzymes of the Calvin-Benson-Bassham pathway of CO2 fixation are often found in clusters that are transcribed from a single promoter under control of the LysR-type transcriptional activator, CbbR. Mutations affecting CbbR prevent induction of cbb gene...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050369
更新日期：1996-09-01 00:00:00
abstract：:Bacillus thuringiensis (Bt) is efficient, strongly specific, and avirulent to humans, making it one of the most popular biopesticides in the world. Bt LLP29 is a mosquitocidal strain that was first isolated from Magnolia denudata. To understand its molecular mechanism against mosquitoes, the genome of Bt LLP29 was seq...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01875-2
更新日期：2020-09-01 00:00:00
abstract：:Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1247-z
更新日期：2016-11-01 00:00:00
abstract：:Purple sulfur bacterium Thiocapsa roseopersicina strain BBS requiring vitamin B12 may grow in the dark in media containing no other organic compounds. Under such conditions the cells oxidize sulfide and thiosulfate with the use of O2 and assimilate carbon dioxide. After 10--30s assimilation of NaH14CO3 about 60% of ra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454854
更新日期：1976-07-01 00:00:00
abstract：:Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00444661
更新日期：1989-01-01 00:00:00
abstract：:In studies on the membranlytic action of various saponins on mycelium of Botrytis cinerea and Rhizoctonia solani digitonin, alpha-hederin and tomatin caused considerable leakage of free amino acids, while aescin and theasaponin were less effective. Cyclamin significantly damaged cell membranes of R. solani, but did no...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447315
更新日期：1975-06-22 00:00:00
abstract：:Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0457-z
更新日期：2009-04-01 00:00:00
abstract：:Two intestinal bacterial strains MT4s-5 and MT42 involved in the degradation of (-)-epigallocatechin (EGC) were isolated from rat feces. Strain MT4s-5 was tentatively identified as Adlercreutzia equolifaciens. This strain converted EGC into not only 1-(3, 4, 5-trihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1006-y
更新日期：2014-10-01 00:00:00
abstract：:Xanthan-deficient mutants of Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, were generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, GumD. Mutants of gumD did not produce xanthan and remained pathogenic in citrus pl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0227-8
更新日期：2007-08-01 00:00:00
abstract：:The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050505
更新日期：1997-10-01 00:00:00
abstract：:The biological effects of UV radiation of different wavelengths (UVA, UVB and UVC) were assessed in nine bacterial isolates displaying different UV sensitivities. Biological effects (survival and activity) and molecular markers of oxidative stress [DNA strand breakage (DSB), generation of reactive oxygen species (ROS)...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0847-5
更新日期：2013-01-01 00:00:00
abstract：:The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough consists of six genes (hmcA to hmcF) that encode structural components of the high-molecular-mass cytochrome redox protein complex (the Hmc complex). Two genes (rrf1 and rrf2) encoding regulatory proteins are present downstream of hmcF. Expression ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050458
更新日期：1997-06-01 00:00:00
abstract：:An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050745
更新日期：1999-08-01 00:00:00
abstract：:Pyrophosphate (PPi) content of Escherichia coli is increased manyfold when the growth of the cells is limited by inhibition of the synthesis of nucleotides. The accumulated PPi is immediately degraded when inhibition is released and growth allowed to resume. Other tested nutritional deficiencies (starvation of carbon ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409846
更新日期：1983-11-01 00:00:00
abstract：:Acyl carrier protein (ACP) is a small acidic protein that acts as an essential cofactor in many biosynthetic pathways depending on acyl transfer reactions. In this work, a Vibrio anguillarum ACP encoding gene, acpV, was first cloned from the chromosome of a virulent V. anguillarum strain MVM425. acpV was over-expresse...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0058-4
更新日期：2006-03-01 00:00:00
abstract：:A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050308
更新日期：1996-02-01 00:00:00
abstract：:The diversity among 45 cyanobacterial isolates from 11 different Gunnera species originating from different geographical areas was examined. By means of polymerase chain reaction (PCR) fingerprinting with short tandemly repeated repetitive (STRR) sequences as primers, ten groups of symbiotic cyanobacteria and five uni...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002039900113
更新日期：2000-02-01 00:00:00
abstract：:The efficiency of hydrolysis of fungal (Fusarium spp.) cell wall and rye root cell wall by crude enzymatic complexes from (42-day-old) cultures of three F. culmorum isolates, a plant growth-promoting rhizosphere isolate (PGPF) DEMFc2, a deleterious rhizosphere isolate (DRMO) DEMFc5, and a pathogenic isolate DEMFc37, a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0803-4
更新日期：2012-08-01 00:00:00
abstract：:Candida utilis grew on ethanol and an ethanol-isopropanol-water (22:2:1 vols) mixture but not on isopropanol alone. Acetone accumulated in all cultures containing isopropranol but its presence in the alcohol mixture did not lower growth rate or yield significantly when compared with growth experiments on ethanol alone...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00403209
更新日期：1980-03-01 00:00:00
abstract：:Although, there have been many published bacterial strains aerobically degrading the heterocyclic amine compounds, only one strain to date has been reported to degrade pyrrolidine under denitrifying conditions. In this study, denitrifying bacteria degrading pyrrolidine and piperidine were isolated from diverse geologi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0452-9
更新日期：2009-04-01 00:00:00
abstract：:The fast-growing Rhizobium sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of Sinorhizobium fredii, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isol...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0559-y
更新日期：2003-07-01 00:00:00
abstract：:The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0971-5
更新日期：2014-05-01 00:00:00
abstract：:The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was de...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
abstract：:1. Culture filtrates of heterotrophic bacteria were tested for their stimulatory effect on nitrification of three strains of Nitrobacter. 2. Yeast extract-peptone solution, in which Pseudomonas fluorescens had grown, after removal of the cells was added to autotrophically growing cultures of Nitrobacter agilis; it cau...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454856
更新日期：1976-07-01 00:00:00
abstract：:1. Mutants derived from the hydrogen bacterium Alcaligenes eutrophus strain H 16 auxotrophic for phenylalanine and tyrosine were isolated employing mutagenic agents (EMS, nitrite), the colistine counterselection technique and the "pin-point" isolation method. Three different types of mutants were found: (1) Mutants, r...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436341
更新日期：1975-04-07 00:00:00
abstract：:Citrobacter rodentium (Cr) is a mouse pathogen that mimics many aspects of enteropathogenic Escherichia coli infections including producing attaching and effacing (A/E) lesions. Host-adapted (HA) Cr cells that are shed at the peak of infection have been reported to be hyper-infective. The exact mechanism underlying th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1191-y
更新日期：2016-05-01 00:00:00
abstract：:Triacylglycerol granules synthesized and accumulated by Rhodococcus opacus and Rhodococcus ruber were isolated by glycerol density gradient centrifugation. Whereas only one type of granule could be isolated from R. opacus, two types of granules with different specific densities were isolated from R. ruber. Both types ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-001-0355-5
更新日期：2001-12-01 00:00:00
