Abstract：
:The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-alanine. Proteomic analysis of the alkaline extracts of the spore proteins, which include exosporium and coat proteins, confirmed that Alr was present in the B. cereus DSA 1 spores and matched to that encoded by B. cereus ATCC 14579, whose spore germination was strongly affected by the block of conversion of L- to D-alanine. Unlike ATCC 14579 spores, L-alanine germination of B. cereus DSA 1 spores was not affected by the preincubation with DCS, suggesting a lack of restriction in the reactant accessibility.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Venir E,Del Torre M,Cunsolo V,Saletti R,Musetti R,Stecchini MLdoi
10.1007/s00203-013-0946-ysubject
Has Abstractpub_date
2014-02-01 00:00:00pages
79-85issue
2eissn
0302-8933issn
1432-072Xjournal_volume
196pub_type
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