Abstract：
:The activities of NAD+-photoreduction and NADH/decyl-ubiquinone reductase in membrane preparations of Rhodobacter capsulatus changed to the same extent under different conditions. These results indicated that NADH:ubiquinone oxidoreductase (complex I) catalyzes the electron transport in the downhill direction (respiratory chain) and in the uphill direction (reverted electron flow). This conclusion was confirmed by the characterization of a complex-I-deficient mutant of R. capsulatus. The mutant was not able to reduce NAD+ in the light. Since this mutant was not able to grow photoautotrophically, we concluded that complex I is the enzyme that catalyzes the reverted electron flow to NAD+ to provide reduction equivalents for CO2 fixation. Complex I is not essential for the reverted electron flow to nitrogenase since the mutant grew under nitrogen-fixing conditions. As shown by immunological means, NuoE, a subunit of complex I from R. capsulatus having an extended C-terminus, was modified depending on the nitrogen source present in the growth medium. When the organism used N2 instead of NH4+, a smaller NuoE polypeptide was synthesized. The complex-I-deficient mutant was not able to modify NuoE. The function of the modification is discussed.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Herter SM,Kortlüke CM,Drews Gdoi
10.1007/s002030050548subject
Has Abstractpub_date
1998-02-01 00:00:00pages
98-105issue
2eissn
0302-8933issn
1432-072Xjournal_volume
169pub_type
杂志文章abstract：:A Gram-stain-negative, aerobic, non-motile and yellow-colored bacterium, strain 17J57-3 T, was isolated from soil collected in Pyeongchang city, Korea. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain 17J57-3 T formed a distinct lineage within the family Oxalobacteraceae (order Burkholderial...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02076-7
更新日期：2020-10-15 00:00:00
abstract：:The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharide (KPS) biosynthesis, is constituted by the rkpU, rkpAGHIJ, and kpsF3 genes. Two mutants in this region affecting the rkpA (SVQ536) and rkpI (SVQ538) genes were constructed. Polyacrylamide gel electrophoresi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0729-2
更新日期：2012-02-01 00:00:00
abstract：:Aerobic anoxygenic phototrophs (AAPs) are prokaryotic microorganisms capable of harvesting light using bacteriochlorophyll-based reaction centres. Marine AAP communities are generally dominated by species belonging to the Roseobacter clade. For this reason, we used marine Roseobacter-related strain COL2P as a model or...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0529-0
更新日期：2010-01-01 00:00:00
abstract：:Soils are rich in organics, particularly those that support the growth of plants. These organics are possible sources of sustainable energy, and a microbial fuel cell (MFC) system can potentially be used for this purpose. In this, the soil organic content expelled from plant root was possibly converted into electrical...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01947-3
更新日期：2020-10-01 00:00:00
abstract：:The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100286
更新日期：2001-07-01 00:00:00
abstract：:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428588
更新日期：1977-05-13 00:00:00
abstract：:Halophilic archaeal strain ZS-47-ST was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-ST was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl2 (optimum 0.03...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1420-z
更新日期：2017-12-01 00:00:00
abstract：:Peptidoglycan of Corynebacterium species Co 112 (DSM 20606) exhibits an unknown amino acid. The amino acid was isolated from cell wall hydrolysates and identified as threo-beta-hydroxyornithine. This amino acid is found in the interpeptide bridge of the peptidoglycan of Corynebacterium sp. Co 112. The primary structur...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407766
更新日期：1983-06-01 00:00:00
abstract：:The determination of enzymatic activities in cell-free extracts of Acidaminococcus fermentans and Peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (R)-2-hydroxyglutarate to acetate and butyrate. From the ratio of these products the amount of ATP generated by sub...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447055
更新日期：1985-07-01 00:00:00
abstract：:Present study attempts in revealing taxonomic and functional diversity of microorganism from petroleum muck using metagenomics approach. Using Ion Torrent Personal Genome Machine, total of 249 Mb raw data were obtained which was analysed using MG-RAST platform. The taxonomic analysis revealed predominance of Proteobac...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0992-0
更新日期：2014-08-01 00:00:00
abstract：:The gliding bacterium Herpetosiphon giganteus Hp a2 was shown to contain gamma-carotene, one monoglucosyloxy and two new diglucosyloxy carotenoids with a x-O-acyldiglucosyloxy carotenoid as main component. Flexirubin-like pigments could not be detected in this organism. As in the Myxobacterales and Cytophagales (Klein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413098
更新日期：1977-04-01 00:00:00
abstract：:AMP-degrading pathways in Azotobacter vinelandii cells were investigated. AMP nucleosidase (EC 3.2.2.4) was rapidly synthesized and reached a maximum at 24 h, while the activity of 5'-nucleotidase (EC 3.1.3.5) specific for AMP, which was negligible during the logarithmic phase of the growth, first appeared in 24 h-cul...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406162
更新日期：1980-12-01 00:00:00
abstract：:Purple sulfur bacterium Thiocapsa roseopersicina strain BBS requiring vitamin B12 may grow in the dark in media containing no other organic compounds. Under such conditions the cells oxidize sulfide and thiosulfate with the use of O2 and assimilate carbon dioxide. After 10--30s assimilation of NaH14CO3 about 60% of ra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454854
更新日期：1976-07-01 00:00:00
abstract：:The association of nucleoside triphosphate molecules and calcium ions with purified particles of mycobacteriophage 13 has been documented. The content of nucleoside triphosphate has been determined to be 118 molecules per phage particle by equilibrium dialysis against labelled ATP or 148 molecules per phage particle b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00527071
更新日期：1981-09-01 00:00:00
abstract：:The starvation survivability of seven Thermococcus strains isolated from four Japanese oil reservoirs was compared with that of Thermococcus strains from marine hydrothermal fields. 16S rDNA analyses showed the isolates to be closely related to Thermococcus litoralis. Growth of the isolates was dependent on amino acid...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100318
更新日期：2001-10-01 00:00:00
abstract：:Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and f...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050713
更新日期：1999-04-01 00:00:00
abstract：:A novel pathway for 2-aminophenol metabolism by Pseudomonas sp. AP-3 is proposed. The proposed pathway is similar to that known for meta-cleavage of catechol except that one of the hydroxyl groups on the metabolites is replaced by an amino group. During the degradation of 2-aminophenol, 2-amino-2,4-pentadienoic acid i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050624
更新日期：1998-08-01 00:00:00
abstract：:A novel Gram-negative rod, endophytic bacterium, designated strain TMCC 8258T, was isolated from the root of Camellia sinensis collected from Puer, south-west China. Comparative 16S rRNA gene sequence analysis showed that the strain belongs to the family Sphingobacteriaceae and a neighbour-joining phylogenetic tree su...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1391-0
更新日期：2017-11-01 00:00:00
abstract：:As the protein databases continue to expand at an exponential rate, fed by daily uploads from multiple large scale genomic and metagenomic projects, the problem of assigning a function to each new protein has become the focus of significant research interest in recent times. Herein, we review the most recent advances ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-010-0549-9
更新日期：2010-03-01 00:00:00
abstract：:Cell free extracts prepared from exponentially growing Escherichia coli and Bacillus cereus as well as from Bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with Bacillus ribonucleic acid. All three preparations synthesized glutam...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00455354
更新日期：1981-04-01 00:00:00
abstract：:The differential gene transcription of a deep-sea bacterium Shewanella piezotolerans WP3 in response to cold shock was analyzed by RNA arbitrarily primed PCR. Ninety primer sets were used to scan two different RNA pools derived from the culture of cold shock and its control (culture at its optimal grown temperature). ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0347-1
更新日期：2008-06-01 00:00:00
abstract：:When Clostridium formicoaceticum was grown on fumarate or L-malate crude cell extracts contained a high fumarate reductase activity. Using reduced methyl viologen as electron donor the specific activity amounted to 2-3.5 U per mg of protein. Reduced benzyl viologen, FMNH2 and NADH could also serve as electron donors b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407920
更新日期：1978-10-04 00:00:00
abstract：:A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were reco...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410734
更新日期：1984-04-01 00:00:00
abstract：:Type I signal peptidases are responsible for the proteolytic cleavage of the signal peptide of secreted proteins. In the gram-positive bacterium Streptomyces lividans, four adjacent genes (sipW, sipX, sipY and sipZ) were isolated encoding putative type I signal peptidases. In this work, the different sip genes were cl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100335
更新日期：2001-11-01 00:00:00
abstract：:Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titrat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02154-w
更新日期：2021-01-05 00:00:00
abstract：:A new psychrophilic, anaerobic, acetogenic bacterium from the tundra wetland soil of Polar Ural is described. The organism fermented H2/CO2, formate, methanol, and several sugars to acetate as the sole end-product. The temperature range for growth was 1-30 degrees C with an optimum at 20 degrees C. The bacterium showe...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000229
更新日期：2000-12-01 00:00:00
abstract：:We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca(2+)-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0498-3
更新日期：2009-09-01 00:00:00
abstract：:Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00444661
更新日期：1989-01-01 00:00:00
abstract：:A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 218...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01774-1
更新日期：2020-04-01 00:00:00
abstract：:Cells of the yeast Saccharomycopsis capsularis fused in pairs after disolving of part of the cross wall between them near the lateral wall. After nuclear migrations through the opening, the cross wall was closed again and the cells at both sides became asci. The wall of the ascospores developed from a prospore wall. B...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447335
更新日期：1975-08-28 00:00:00
