Abstract：
:Chloroquine inhibited the outgrowth of Bacillus subtilis spores at a 10-fold lower concentration than that required to prevent vegetative growth. Analysis of macromolecular synthesis in outgrowing spores and vegetative cells in the presence of chloroquine indicated that it acted preferentially on transcription. Differential sensitivity of outgrowing spores and vegetative cells to chloroquine was not due to changes in the specificity of the RNA-polymerase, since RNA-polymerase activity measured in permeabilized cells was not affected differently by the drug. The preferential inhibition of spore outgrowth was not evident at pH 8.0, at which the majority of chloroquine is in a monovalent, more lipophilic, form. In the presence of inhibitors affecting membrane potential, vegetative cells were as sensitive to chloroquine as outgrowing spores. Measurement of [14C]-chloroquine uptake showed that early outgrowing spores accumulated twice as much drug as resistant late outgrowing spores and seven times more than vegetative cells. Treatment of vegetative cells with metabolic inhibitors led them to accumulate chloroquine to the levels found in outgrowing spores. Therefore, the preferential inhibition of outgrowing spores by chloroquine is the result of increased uptake of the drug, reflecting differences in energy metabolism from vegetative cells.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Smith KT,Dawes IWdoi
10.1007/BF00409659subject
Has Abstractpub_date
1989-01-01 00:00:00pages
251-7issue
3eissn
0302-8933issn
1432-072Xjournal_volume
152pub_type
杂志文章abstract：:Regulation of transcription of the Escherichia coli moe operon, which codes for proteins connecting molybdate metabolism, molybdopterin synthesis, and apomolybdoenzyme synthesis, was investigated. Expression of the moe operon was independent of genes coding for molybdate transport and Mo-cofactor biosynthesis. Express...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100252
更新日期：2001-03-01 00:00:00
abstract：:Xanthan-deficient mutants of Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, were generated by deletion and marker exchange of the region encoding the carboxy-terminal end of the first glycosyltransferase, GumD. Mutants of gumD did not produce xanthan and remained pathogenic in citrus pl...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0227-8
更新日期：2007-08-01 00:00:00
abstract：:Selenium nanoparticles (Se NPs) were synthesized using Saccharomyces cerevisiae yeast. Influences of different amounts of sodium selenite (5.0, 10.0, 15.0, 20.0, and 25 µg) were evaluated on growth of yeast during incubation at 32 °C, during 4 days. UV-Vis spectroscopy results have shown that synthesized Se NPs had br...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01831-0
更新日期：2020-07-01 00:00:00
abstract：:We have investigated the endogenous phosphorylation patterns of phosphorylated proteins of Xanthomonas campestris pv. oryzae induced by its bacteriophages. For bacteriophage Xp12-infected cells, at least three phosphoproteins with apparent molecular weights of 28, 28.5 and 45 kDa were detected by in vitro labeling wit...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00303581
更新日期：1994-01-01 00:00:00
abstract：:DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00288947
更新日期：1994-01-01 00:00:00
abstract：:Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using pro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00314469
更新日期：1994-01-01 00:00:00
abstract：:The divergently transcribed sulfur oxidation (sox) operon of a sulfur chemolithotrophs, Pseudaminobacter salicylatoxidans KCT001, comprising sox TRS-VW-XYZABCD, is regulated by a repressor (SoxR). SoxR binds to two disparate operators, sv (present in between soxS and soxV) and wx (present in between soxW and soxX). He...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0808-z
更新日期：2012-09-01 00:00:00
abstract：:The site of integration of phage M mu d (Ap lac) in mutant M9s which leads to deficiency of formic dehydrogenase (benzylviologen-linked) activity was determined. It was shown that the phage had inserted into the gene for the seleno-polypeptide of the enzyme (80 kd) leading to the formation of a truncated peptide (60 k...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428850
更新日期：1985-05-01 00:00:00
abstract：:The synthesis of beta-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-D-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the prese...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447154
更新日期：1975-11-07 00:00:00
abstract：:Colistin is a mixture of polymyxin E1 and E2, bactericidal pentacationic lipopeptides used to treat infections caused by Gram-negative pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae. Industrial production of colistin is obtained by a fermentation process of the natural producer Paenibacillus polymy...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1084-5
更新日期：2015-05-01 00:00:00
abstract：:Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 degrees C in EDTA-free medium. High-molecular-weight mannop...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00307769
更新日期：1994-01-01 00:00:00
abstract：:Yeast suffers from a variety of environmental stresses, such as osmotic pressure and ethanol produced during fermentation. Since calcium ions are protective for high concentrations of ethanol, we investigated whether Ca(2+) flux occurs in response to ethanol stress. We find that exposure of yeast to ethanol induces a ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0673-6
更新日期：2011-05-01 00:00:00
abstract：:The presence of one periplasmic [NiFe] hydrogenase, one periplasmic [Fe] hydrogenase, and one cytoplasmic NADP-reducing hydrogenase has been previously established in Desulfovibrio fructosovorans. In the present work, marker-exchange mutagenesis was performed to determine the function of the tetrameric NADP-reducing h...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050414
更新日期：1997-01-01 00:00:00
abstract：:Pseudomonas cepacia grew rapidly using a mixture of all three branched chain amino acids as carbon source, but failed to use individual branched chain amino acids as sole carbon source. Extracts of bacteria grown on branched chain amino acids had between 2- and 3-fold higher levels of alpha-ketoglutarate-dependent bra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00423069
更新日期：1979-03-12 00:00:00
abstract：:Present study attempts in revealing taxonomic and functional diversity of microorganism from petroleum muck using metagenomics approach. Using Ion Torrent Personal Genome Machine, total of 249 Mb raw data were obtained which was analysed using MG-RAST platform. The taxonomic analysis revealed predominance of Proteobac...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0992-0
更新日期：2014-08-01 00:00:00
abstract：:Video-enhanced light microscopy of the apical and subapical regions of growing hyphae of several fungal species revealed the existence of momentary synchronized motions of subcellular organelles. First discovered in a temperature-sensitive morphological mutant (ramosa-1) of Aspergillus niger, these seemingly spontaneo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0771-z
更新日期：2005-05-01 00:00:00
abstract：:Penicillium marneffei is a dimorphic fungus that intracellularly infects the reticuloendothelial system of humans and bamboo rats. Endemic in Southeast Asia, it infects 10% of AIDS patients in this region. The absence of a sexual stage and the highly infectious nature of the mould-phase conidia have impaired studies o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0533-8
更新日期：2003-05-01 00:00:00
abstract：:Previous work has shown that the mechanosensitive (MS) channel of large conductance (MscL) is essential for preventing lysis of Bacillus subtilis log phase cells upon a rapid, severe osmotic downshock. Growing cells of B. subtilis strains lacking MscL and one or more putative MS channel proteins of small conductance (...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0292-z
更新日期：2008-01-01 00:00:00
abstract：:The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454864
更新日期：1989-01-01 00:00:00
abstract：:Recent studies indicated that bioluminescence of the marine bacterium Vibrio harveyi may both stimulate DNA repair and contribute to detoxification of deleterious oxygen derivatives. Therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0764-y
更新日期：2005-03-01 00:00:00
abstract：:Nitrosomonas europaea, a Gram-negative obligate chemolithoautotroph, participates in global nitrogen cycling by carrying out nitrification and derives energy for growth through oxidation of ammonia. In this work, the physiological, proteomic, and transcriptional responses of N. europaea to zinc stress were studied. Th...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0341-7
更新日期：2008-06-01 00:00:00
abstract：:Utilization of D-amino acids being substrates of D-amino acid dehydrogenase of Salmonella typhimurium was examined. The experiments were done with wild type strains and the mutants dadA missing the enzyme activity and dadR in which its synthesis is released from catabolite repression. Growth on D-tryptophan, D-histidi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406077
更新日期：1978-07-01 00:00:00
abstract：:The metabolism of methylamine as the nitrogen source for growth of the non-methylotrophic yeast Candida utilis and the methylotrophic yeast Hansenula polymorpha was investigated. Growth of both organisms in media with glucose and methylamine was associated with the presence of an amine oxidase in these cells. The enzy...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00511216
更新日期：1980-06-01 00:00:00
abstract：:We previously reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions do not. This finding suggests that Ptp2p and Msg5p are involved in Ca(2+)-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0498-3
更新日期：2009-09-01 00:00:00
abstract：:The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00423075
更新日期：1979-03-12 00:00:00
abstract：:We report structural and functional analyses of the Bradyrhizobium japonicum fixGHIS genes, which map immediately downstream of the fixNOQP operon for the symbiotically essential cbb3-type heme-copper oxidase complex. Expression of fixGHIS, like that of fixNOQP, is strongly induced in cells grown microaerobically or a...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050330
更新日期：1996-05-01 00:00:00
abstract：:Streptococcus intermedius is a causative agent of brain or liver abscesses. S. intermedius produces intermedilysin that plays a pivotal role in pathogenicity. We identified other pathogenic factors and described a fibronectin binding protein (FBP) homolog of S. intermedius (FbpI) that mediated bacterial adhesion to ep...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01922-y
更新日期：2020-10-01 00:00:00
abstract：:In this study, a Candida digboiensis strain was isolated from a heap leaching plant in Zambia and used in double-layer agar plate to efficiently isolate and purify leaching bacteria. Unlike Acidiphilium sp., the yeast strain was tetrathionate tolerant and could metabolize a great range of organic compounds including o...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1051-6
更新日期：2015-03-01 00:00:00
abstract：:Cryptococcosis is a common opportunistic infection in patients with advanced HIV infection and may also affect immunocompetent patients. The available antifungal agents are few and other options are needed for the cryptococcosis treatment. In this work, we first analyzed the virulence of twelve C. neoformans and C. ga...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01789-8
更新日期：2020-05-01 00:00:00
abstract：:Rather recently it has become clear that prokaryotes (Archaea and Bacteria) are able to glycosylate proteins. A literature survey revealed the different types of glycoproteins. They include mainly surface layer (S-layer) proteins, flagellins, and polysaccharide-degrading enzymes. Only in a few cases is structural info...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s002030050484
更新日期：1997-09-01 00:00:00
