Utilization of D-amino acids by dadR mutants of Salmonella typhimurium.

Abstract:

:Utilization of D-amino acids being substrates of D-amino acid dehydrogenase of Salmonella typhimurium was examined. The experiments were done with wild type strains and the mutants dadA missing the enzyme activity and dadR in which its synthesis is released from catabolite repression. Growth on D-tryptophan, D-histidine and D-methionine used as precursors of the L-amino acids was faster when the respective auxotrophs carried dadR mutations. The dadR mutants grew faster when D-or L-alanine was present as a sole source of nitrogen. Experiments with D-amino acid dehydrogenase in vitro provided evidence that D-tryptophan is its substrate with a very low affinity to the dehydrogenase.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Wild J,Filutowicz M,Kłopotowski T

doi

10.1007/BF00406077

subject

Has Abstract

pub_date

1978-07-01 00:00:00

pages

71-7

issue

1

eissn

0302-8933

issn

1432-072X

journal_volume

118

pub_type

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