Abstract：
:Yeast suffers from a variety of environmental stresses, such as osmotic pressure and ethanol produced during fermentation. Since calcium ions are protective for high concentrations of ethanol, we investigated whether Ca(2+) flux occurs in response to ethanol stress. We find that exposure of yeast to ethanol induces a rise in the cytoplasmic concentration of Ca(2+). The response is enhanced in cells shifted to high-osmotic media containing proline, galactose, sorbitol, or mannitol. Suspension of cells in proline and galactose-containing media increases the Ca(2+) levels in the cytoplasm independent of ethanol exposure. The enhanced ability for ethanol to induce Ca(2+) flux after the hypertonic shift is transient, decreasing rapidly over a period of seconds to minutes. There is partial recovery of the response after zymolyase treatment, suggesting that cell wall integrity affects the ethanol-induced Ca(2+) flux. Acetate inhibits the Ca(2+) accumulation elicited by the ethanol/osmotic stress. The Ca(2+) flux is primarily via the Cch1 Ca(2+) influx channel because strains carrying deletions of the cch1 and mid1 genes show greater than 90% reduction in Ca(2+) flux. Furthermore, a functional Cch1 channel reduced growth inhibition by ethanol.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Courchesne WE,Vlasek C,Klukovich R,Coffee Sdoi
10.1007/s00203-010-0673-6subject
Has Abstractpub_date
2011-05-01 00:00:00pages
323-34issue
5eissn
0302-8933issn
1432-072Xjournal_volume
193pub_type
杂志文章abstract：:The mechanism of lysis of Eubacterium alactolyticum cell walls by Streptomyces albus G enzyme was studied. The analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of S. albus G enzyme was mainly du...
journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492896
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journal_title：Archives of microbiology
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更新日期：2012-07-01 00:00:00
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更新日期：2009-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1976-04-01 00:00:00
