Localization of aggregation substances of Enterococcus faecalis after induction by sex pheromones. An ultrastructural comparison using immuno labelling, transmission and high resolution scanning electron microscopic techniques.

Abstract:

:The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electron microscopy and high resolution scanning electron microscopy we investigated the appearance and distribution of the aggregation substance. The "hairs" increase in number with increasing exposure to sex pheromones (maximum density: 1300/microns2). We show that these structures are unequally distributed over the cell surface, even if the cells were induced by sex pheromones for a long period of time. Statistical analysis of the unequal distribution indicates that aggregation substance is incorporated into pre-existing "old" cell-walls and that this incorporation shows a saturation ca. 40 min after addition of sex pheromones.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Wanner G,Formanek H,Galli D,Wirth R

doi

10.1007/BF00454864

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

491-7

issue

6

eissn

0302-8933

issn

1432-072X

journal_volume

151

pub_type

杂志文章
  • Plant growth promotion traits of phosphobacteria isolated from Puna, Argentina.

    abstract::The ability of soil microorganisms to solubilize phosphate is an important trait of plant growth-promoting bacteria leading to increased yields and smaller use of fertilizers. This study presents the isolation and characterization of phosphobacteria from Puna, northwestern Argentina and the ability to produce phosphat...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-011-0692-y

    authors: Viruel E,Lucca ME,Siñeriz F

    更新日期:2011-07-01 00:00:00

  • Sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.

    abstract::Recent studies indicated that bioluminescence of the marine bacterium Vibrio harveyi may both stimulate DNA repair and contribute to detoxification of deleterious oxygen derivatives. Therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolu...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-005-0764-y

    authors: Lyzeń R,Wegrzyn G

    更新日期:2005-03-01 00:00:00

  • Further studies on RpoS in enterobacteria: identification of rpoS in Enterobacter cloacae and Kluyvera cryocrescens.

    abstract::RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacte...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030100277

    authors: Martínez-García E,Tormo A,Navarro-Llorens JM

    更新日期:2001-06-01 00:00:00

  • Lactate dehydrogenases in cyanobacteria.

    abstract::NAD-linked lactate dehydrogenases specific for the D- and L-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied. The enzyme has a molecular weight of ca. 115000-120000...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00447300

    authors: Sanchez JJ,Palleroni NJ,Doudoroff M

    更新日期:1975-06-20 00:00:00

  • Metabolic regulation of pyruvate kinase isolated from autotrophically and heterotrophically grown Paracoccus denitrificans.

    abstract::Pyruvate kinase (ATP: pyruvate phosphotransferase (EC 2.7.1.40) was partially purified from both autotrophically and heterotrophically grown Paracoccus denitrificans. The organism grown under heterotrophic conditions contains four times more pyruvate kinase than under autotrophic conditions. The enzyme isolated from b...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00427847

    authors: Slabas AR,Whatley FR

    更新日期:1977-10-24 00:00:00

  • Identification of new enzymes potentially involved in anaerobic naphthalene degradation by the sulfate-reducing enrichment culture N47.

    abstract::The sulfate-reducing highly enriched culture N47 is capable to anaerobically degrade naphthalene, 2-methylnaphthalene, and 2-naphthoic acid. A proteogenomic investigation was performed to elucidate the initial activation reaction of anaerobic naphthalene degradation. This lead to the identification of an alpha-subunit...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-010-0667-4

    authors: Bergmann FD,Selesi D,Meckenstock RU

    更新日期:2011-04-01 00:00:00

  • Purification, partial characterization, and subcellular localization of a 38 kilodalton, calcium-regulated protein of Rhizobium fredii USDA208.

    abstract::Calcium is essential for the growth of rhizobia and the formation of nitrogen-fixing root-nodules on legumes, but its precise role in these processes remains unknown. We have found that Rhizobium fredii USDA208 accumulates a major 38 kDa protein when grown in media supplemented with 0.3-2 microM Cacl2. We have purifie...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00248480

    authors: Krishman HB,Pueppke SG

    更新日期:1993-01-01 00:00:00

  • Glycoproteins in prokaryotes.

    abstract::Rather recently it has become clear that prokaryotes (Archaea and Bacteria) are able to glycosylate proteins. A literature survey revealed the different types of glycoproteins. They include mainly surface layer (S-layer) proteins, flagellins, and polysaccharide-degrading enzymes. Only in a few cases is structural info...

    journal_title:Archives of microbiology

    pub_type: 杂志文章,评审

    doi:10.1007/s002030050484

    authors: Moens S,Vanderleyden J

    更新日期:1997-09-01 00:00:00

  • Anaplerotic metabolism of Aspergillus nidulans and its effect on biomass synthesis in carbon limited chemostats.

    abstract::Anaplerotic fixation of carbon dioxide by the fungus Aspergillus nidulans when grown under carbon-limited conditions was mediated by pyruvate carboxylase and a phosphoenol pyruvate (PEP)-metabolising enzyme which has been tentatively designated as PEP carboxylase. The activities of both enzymes were growth rate depend...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00422531

    authors: Bushell ME,Bull AT

    更新日期:1981-01-01 00:00:00

  • Changes in phosphatase activity associated with cell wall defects in Chlamydomonas reinhardi.

    abstract::Experiments were performed to isolate mutants lacking alkaline phosphatase in Chlamydomonas reinhardi. Mutants with null enzyme activity were obtained. A cytological study of these mutants however revealed cell wall defects, suggesting that the loss of phosphatase activity in these strains is not due to the inactivati...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00436357

    authors: Loppes R,Deltour R

    更新日期:1975-05-05 00:00:00

  • Morphogenesis and alpha-tubulin gene of plasmodium in Didymium megalosporum.

    abstract::The morphogenesis of plasmodium in Didymium megalosporum was observed for the first time by hanging drop culture and 3 % oat-agar culture under controlled conditions. The development of plasmodium was characteristic formation process of phaneroplasmodium. The mature plasmodium was white yellow or yellow green in color...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-014-0971-5

    authors: Song X,Zhong H,Wang Q,Li Y

    更新日期:2014-05-01 00:00:00

  • Transport of branched-chain amino acids in Corynebacterium glutamicum.

    abstract::The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 micro...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00413136

    authors: Ebbighausen H,Weil B,Krämer R

    更新日期:1989-01-01 00:00:00

  • Searching for putative virulence factors in the genomes of Shewanella indica and Shewanella algae.

    abstract::Bacterial pathogens are a major threat to both humans and animals worldwide. It is crucial to understand the mechanisms of various disease processes at the molecular level. Shewanella species are widespread in the environment and some are considered as emerging opportunistic human and marine mammal pathogens. In this ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-02060-1

    authors: Tamez AM,McLaughlin RW,Li J,Wan X,Zheng J

    更新日期:2020-10-10 00:00:00

  • Sophorose induction of an intracellular b-glucosidase in Trichoderma.

    abstract::The disaccharide sophorose induces Trichoderma to increase a soluble intracellular b-glucosidase that hydrolyses cellobiose, sophorose, and p-nitrophenyl-b-D-glucopyranoside. Simultaneously, it depresses the activity of a similar insoluble enzyme that is associated with the mycelium. Gel electrophoresis indicates that...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00425807

    authors: Loewenberg JR

    更新日期:1984-01-01 00:00:00

  • Ethanol-sensitive mutants of Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00412799

    authors: Aguilera A,Benítez T

    更新日期:1986-01-01 00:00:00

  • Structural analysis of cell wall mannan of Candida sojae, a new yeast species isolated from defatted soybean flakes.

    abstract::We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-007-0339-1

    authors: Oyamada H,Ogawa Y,Shibata N,Okawa Y,Suzuki S,Kobayashi H

    更新日期:2008-05-01 00:00:00

  • Genome-wide transcriptional responses of Nitrosomonas europaea to zinc.

    abstract::Nitrosomonas europaea, a Gram-negative obligate chemolithoautotroph, participates in global nitrogen cycling by carrying out nitrification and derives energy for growth through oxidation of ammonia. In this work, the physiological, proteomic, and transcriptional responses of N. europaea to zinc stress were studied. Th...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-007-0341-7

    authors: Park S,Ely RL

    更新日期:2008-06-01 00:00:00

  • Sphingomonas hankyongensis sp. nov. isolated from tap water.

    abstract::A Gram reaction-negative, strictly aerobic, non-motile, translucent and rod-shaped bacterium (designated W1-2-4(T)) isolated from tap water was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-4(T) was observed to grow optimally at 25-30 °C and at pH 6.5 on nutrient agar. Phylogene...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-016-1237-1

    authors: Yun SS,Siddiqi MZ,Lee SY,Kim MS,Choi K,Im WT

    更新日期:2016-10-01 00:00:00

  • The characterization of the nv-gvpACNOFGH gene cluster involved in gas vesicle formation in Natronobacterium vacuolatum.

    abstract::The haloalkaliphilic archaeon Natronobacterium vacuolatum forms cylinder-shaped gas vesicles throughout the growth cycle when grown in media containing 15-25% NaCl. Cells cultivated in media containing 13% NaCl are, however, gas-vesicle-free. The major gas vesicle structural protein, nv-GvpA, was detected by an antise...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030050465

    authors: Mayr A,Pfeifer F

    更新日期:1997-07-01 00:00:00

  • Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species.

    abstract::Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR enc...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030100350

    authors: Gustafsson MC,Palmer CN,Wolf CR,von Wachenfeldt C

    更新日期:2001-12-01 00:00:00

  • Elucidation of the pathways of catabolic glutamate conversion in three thermophilic anaerobic bacteria.

    abstract::The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s002030100286

    authors: Plugge CM,van Leeuwen JM,Hummelen T,Balk M,Stams AJ

    更新日期:2001-07-01 00:00:00

  • The UvrY response regulator of the BarA-UvrY two-component system contributes to Yersinia ruckeri infection of rainbow trout (Oncorhynchus mykiss).

    abstract::To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-010-0582-8

    authors: Dahiya I,Stevenson RM

    更新日期:2010-07-01 00:00:00

  • The seleno-polypeptide of formic dehydrogenase (formate hydrogen-lyase linked) from Escherichia coli: genetic analysis.

    abstract::The site of integration of phage M mu d (Ap lac) in mutant M9s which leads to deficiency of formic dehydrogenase (benzylviologen-linked) activity was determined. It was shown that the phage had inserted into the gene for the seleno-polypeptide of the enzyme (80 kd) leading to the formation of a truncated peptide (60 k...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00428850

    authors: Pecher A,Zinoni F,Böck A

    更新日期:1985-05-01 00:00:00

  • A serine alkaline protease from the fungus Conidiobolus coronatus with a distinctly different structure than the serine protease subtilisin Carlsberg.

    abstract::In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF01682989

    authors: Phadtare S,Rao M,Deshpande V

    更新日期:1996-12-01 00:00:00

  • Congo Red affects the growth, morphology and activity of glucosamine-6-phosphate synthase in the human pathogenic fungus Sporothrix schenckii.

    abstract::Sporothrix schenckii is the etiological agent of sporotrichosis, a mycosis of humans and other mammals. Little is known about the responses of this thermodimorphic pathogen to perturbations in the cell wall (CW) by different stress conditions. Here we describe the effect of Congo Red (CR) on the fungal growth, morphog...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-018-1576-1

    authors: Sánchez-López JF,González-Ibarra J,Macías-Segoviano JI,Cuéllar-Cruz M,Álvarez-Vargas A,Cano-Canchola C,López-Romero E

    更新日期:2019-01-01 00:00:00

  • Different polysaccharides in the external layers (capsule and slime) of the cell envelope of Rhodopseudomonas capsulata Sp11.

    abstract::Two different acidic polysaccharides (I and II) were detected in the external cell envelope layers (slime and capsule) of Rhodopseudomonas capsulata Sp11. Polysaccharide I contains rhamnose, fucose, glucosamine and an unknown acidic sugar, it represents the slime material of the strain. Polysaccharide II contains rham...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00425219

    authors: Omar AS,Weckesser J,Mayer H

    更新日期:1983-12-01 00:00:00

  • Characterization of a novel T4-type Stenotrophomonas maltophilia virulent phage Smp14.

    abstract::Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-007-0238-5

    authors: Chen CR,Lin CH,Lin JW,Chang CI,Tseng YH,Weng SF

    更新日期:2007-08-01 00:00:00

  • Drought-tolerant and plant growth-promoting endophytic Staphylococcus sp. having synergistic effect with silicate supplementation.

    abstract::Endophytic bacteria have been reported to have symbiotic, mutualistic, commensalistic or trophobiotic relationships with various plant parts. As part of its adaptation, many endophytic organisms are known to exhibit properties with multiple beneficial effects to the plant system. Even though many bacterial genera have...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/s00203-020-01911-1

    authors: Jayakumar A,Krishna A,Nair IC,Radhakrishnan EK

    更新日期:2020-09-01 00:00:00

  • Oxidation of formaldehyde by alcohol oxidase of Candida boidinii.

    abstract::A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the p...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00447134

    authors: Sahm H

    更新日期:1975-10-27 00:00:00

  • Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. II. The isolation and characterization of mutants auxotrophic for phenylalanine and tyrosine.

    abstract::1. Mutants derived from the hydrogen bacterium Alcaligenes eutrophus strain H 16 auxotrophic for phenylalanine and tyrosine were isolated employing mutagenic agents (EMS, nitrite), the colistine counterselection technique and the "pin-point" isolation method. Three different types of mutants were found: (1) Mutants, r...

    journal_title:Archives of microbiology

    pub_type: 杂志文章

    doi:10.1007/BF00436341

    authors: Friedrich B,Schlegel HG

    更新日期:1975-04-07 00:00:00